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Y Sano

Publications and source records attributed to Y Sano.

At least 325 records · Page 18Linked to original sources
PubMed1985

Immuohistochemical demonstration of alpha-atrial natriretic plypeptide-containing neurons in the rat brain.

Using the immunoperoxidase technique in conjunction with specific antisera to alpha-atrial natriuretic polypeptide (alpha-ANP), it was shown that immunoreactive cell bodies and varicose fibers are widely distributed throughout the rat brain. The highest concentrations of alpha-ANP-containing neuronal cell bodies and fibers were found in the hypothalamus and septum. This result confirms the radioimmunological determination of alpha-ANP immunoreactivity in the rat brain.

Animals↗
PubMed1985

The biotinylation of the rabbit serotonin antibody and its application to immunohistochemical studies using the two-step ABC method.

A newly modified and improved immunohistochemical method was devised for the demonstration of the serotonin neuron system in the central nervous system of the rabbit using serotonin antibody obtained by the immunization of rabbits, i.e., the biotinylation of serotonin antibodies, and the application of the two-step avidin-biotin-peroxidase complex (ABC) method. Using this technique, high background staining and nonspecific reactions were avoided, and extremely clear preparations were produced. The serotonin neurons of rabbit brain, which have a Golgi-like appearance, were followed to the fine terminals. This technique were also applied for electron microscopy, and satisfactory results concerning the submicroscopical distribution of serotonin were obtained.

Animals↗
PubMed1985

Immunohistochemical demonstration of serotonin nerve fibers in the corpus striatum of the rat, cat and monkey.

The distribution of serotonin-containing nerve fibers in the corpus striatum of the rat, cat and monkey was studied with modified peroxidase-antiperoxidase method using serotonin antiserum without any pretreatment. In the neostriatum (caudate nucleus and putamen) of all mammalian species investigated, the immunoreactive fibers were distinct varicose fibers in a fine network. The concentration of these fibers was high in the ventral, medial and caudal neostriatum. Especially in the area bounded by the globus pallidus, serotonin fibers were abundant and compactly arranged along the nucleus. In this area of the monkey, a few thick fibers (tract fibers) were intermingled; they ran along the lateral medullary lamina. Such tract fibers were also observed outside the medial medullary lamina and in the central portion of the medial pallidal segment. The paleostriatum (globus pallidus and entopeduncular nucleus) of the rat and cat as well as the medial pallidal segment of the monkey was diffusely innervated with serotonin fibers composed of numerous varicosities and fine intervaricose segments, while in the lateral pallidal segment of the monkey, the distribution of fibers was scanty and partial. Our results hint at the morphological basis of the serotonergic regulation of the extrapyramidal system in mammals.

Animals↗
PubMed1985

The organization of serotonin fibers in the mammalian superior colliculus. An immunohistochemical study.

The distribution of serotonin immunoreactivity in the superior colliculus (SC) of the rat, hamster, chipmunk, cat, and monkey was studied using a sensitive immunohistochemical method. In all of these animals, serotonin immunoreactivity formed a dense network of varicose fibers throughout the SC. These fibers had a characteristic arrangement corresponding to the laminar structures of the SC. Except in the chipmunk, serotonergic fibers were more dense in the stratum griseum superficiale than in the other layers. In the SC of the chipmunk, these fibers appeared evenly distributed. To explore the degree of serotonergic innervation in each layer, a semi-quantitative assay of serotonin immunoreactive varicosities was conducted in the rat, chipmunk, cat, and monkey. Peaks in varicose density were seen in the stratum griseum superficiale, the stratum griseum intermedium and the stratum griseum profundum. In the rat, cat, and monkey, the highest density of these varicosities was in the stratum griseum superficiale. On the other hand, the stratum griseum intermedium of the chipmunk SC received the greatest innervation of serotonergic varicose fibers.

Animals↗
PubMed1985

Immunohistological studies of masked indoleamine cells in the area postrema of the rat.

Masked indoleamine cells (MICS) in the area postrema and adjacent areas in the rat were immunohistochemically studied (the peroxidase-antiperoxidase method) using a serotonin antiserum. After pretreatment with nialamide (200-300 mg/kg), immunoreactive MICS could be observed. They were small cells (about 12 micron in diameter) with several processes and were distributed in nearly all parts of the area postrema and also in the nucleus tructus solitarii. Following a single intraventricular injection of 75 micrograms 5,6-dihydroxytryptamine, the immunoreactivity of these cells conspicuously decreased for several days. The submicroscopical structure of the cells was investigated using immunoelectron microscopy. Immunoreactive products were observed in the cytoplasm as particles with a diameter of 25-40 nm and high electron density, but these were not found in the nucleus or cell organelles.

5,6-Dihydroxytryptamine↗
PubMed1985

A new radioimmunoassay for serum 16 alpha-hydroxyandrost-4-ene-3,17-dione with specific antiserum.

A radioimmunoassay system for serum 16 alpha-hydroxyandrost-4-ene-3,17-dione was developed with the use of rabbit antiserum against 16 alpha-hydroxyandrost-4-ene-3,17-dione-3-(O-carboxymethyl)oxime which was conjugated with bovine serum albumin. The antiserum was highly specific for 16 alpha-hydroxyandrost-4-ene-3,17-dione, with cross reactions to other steroids being less than 0.8% except for androst-4-ene-3,17-dione(3.4% cross reaction). Use of LH-20 column chromatography, however, clearly separated these two steroids. Pregnancy sera were measured with this assay system after an addition of labelled internal standard, extraction and separation by column chromatography. The lower limit of detection for 16 alpha-hydroxyandrost-4-ene-3,17-dione was 2 pg/tube. The mean recovery rate of the added standard was 98.3 +/- 8.8% (mean +/- SE). Intra- and inter-assay coefficients of variation were 8.6% (n = 6) and 12.1% (n = 7), respectively.

Amniotic Fluid↗
PubMed1985

Immunohistochemical investigations of the influence of reserpine on the serotonin neuron system in the rat brain.

A peroxidase-antiperoxidase immunohistochemical method with serotonin antiserum was employed to investigate the influence of reserpine on serotonin neurons of rats which were sacrificed at various times after injection (10 mg/kg i.p.). The disappearance of serotonin immunoreactivity induced by reserpine was detected only in the perikarya after 15 min, and then rapidly proceeded to the terminals. Between 2 and 4 h, immunoreactivity completely disappeared throughout the brain. The immunoreactivity reappeared in the perikarya after 6 h, and progressed toward the terminals gradually. However, there was an obvious difference in the rate of recovery of immunoreactivity between areas. After 7 days, the immunoreactivity returned to control levels.

Animals↗
PubMed1985

Atrial natriuretic polypeptide: topographical distribution in the rat brain by radioimmunoassay and immunohistochemistry.

The widespread distribution of neurons containing alpha-atrial natriuretic polypeptide-like immunoreactivity in the rat brain was demonstrated using radioimmunoassay and immunohistochemistry in conjunction with specific antisera. The highest concentrations of alpha-atrial natriuretic polypeptide-like immunoreactivity were in the hypothalamus and septum, with low but still appreciable concentrations in the mesencephalon, cerebral cortex, olfactory bulb and thalamus by radioimmunoassay. Immunohistochemical studies clearly showed that the perikarya of immunoreactive neurons are most prevalent in the ventral part of the lateral septal nucleus, periventricular preoptic nucleus, bed nucleus of the stria terminalis, periventricular and dorsal parts of the paraventricular hypothalamic nucleus, ventromedial nucleus, dorsomedial nucleus, arcuate nucleus, median mamillary nucleus, supramamillary nucleus, zona incerta, medial habenular nucleus and the periaqueductal grey matter. Scattered neurons were seen in the cingulate cortex, endopiriform nucleus, lateral hypothalamic area, and pretectal and dorsal thalamic areas. In addition to the areas mentioned above, high concentrations of immunoreactive varicose fibers were seen in the glomerular layer of the olfactory bulb, external layer of the median eminence, central to paramedian parts of the interpeduncular nucleus and the paraventricular hypothalamic nucleus. The globus pallidus, medial and central amygdaloid nuclei, dorsal raphe, dorsal parabrachial nucleus, locus coeruleus, vagal dorsal motor nucleus, solitary nucleus and some circumventricular organs, including the subfornical organ and organum vasculosum laminae terminalis, contained considerable numbers of immunoreactive varicose fibers. In dehydrated rats and homozygous Brattleboro rats, the pattern of alpha-atrial natriuretic polypeptide-immunoreactive neurons and varicose fibers was qualitatively similar to that seen in normal conditioned rats. This study gives an atlas of the distribution of the alpha-atrial natriuretic polypeptide-containing neuronal system in the rat brain and provides the groundwork for studying the influence of this new peptide on various brain functions.

Animals↗
PubMed1985

Olivary hypertrophy in a case with palatal myoclonus: light- and electron-microscopic study.

This is a report on the ultrastructural finding of the olivary hypertrophy in a case with palatal myoclonus. By light microscopy two types of neuronal changes were observed in the inferior olivary nucleus, i.e. the central chromatolysis and cytoplasmic vacuolation. Both types were also recognized by electron microscopy and the cytoplasmic vascuolation was identified as the vesiculated endoplasmic reticulum. In the reactive astrocytes, mitochondria were strikingly proliferated.

Adult↗
PubMed1985

Immunohistochemical studies of the serotonergic supraependymal plexus in the mammalian ventricular system, with special reference to the characteristic reticular ramification.

Distributional and morphological features, especially characteristics of the ramification of serotonin-containing supraependymal fibers (SEF), were studied in the ventricular systems of mammals (mouse, rat, guinea pig, rabbit, cat, dog, monkey) by means of a modified peroxidase antiperoxidase technique, using antiserotonin antiserum prepared in our laboratory. SEF were present in all ventricular systems, except on the third ventricle floor and in the choroid plexus. The density of SEF was higher in the smaller species. In the rat, light- and scanning electron microscopical SEF were almost completely abolished 1 week after intraventricular administration of 5,6-dihydroxytryptamine. Ramification of SEF was complicated; the SEF formed a true network with frequent anastomosing. In the ventricular system of rats rendered hydrocephalic by kaolin administration, the mode of axonal branching in the supraependymal plexus could best be analyzed by the scanning electron microscope because the meshes of the plexus were spread out.

Animals↗
PubMed1985

C3 variants in Japanese.

By high-voltage agarose gel electrophoresis, seven phenotypes of C3 were found in Japanese. The allele frequencies for C3*S, C3*S025, C3*S02, C3*F, C3*F06, C3*F065, and C3*F08 were 0.9943, 0.0003, 0.0003, 0.0006, 0.0003, 0.0021, and 0.0021, respectively. CH50, C3/C3c protein concentrations, and C3 hemolytic activities in fresh sera with variant C3 phenotypes were within the normal ranges.

Adult↗
PubMed1985

Distribution of serotonin nerve cells in the rabbit brain--immunohistochemistry by the two-step ABC technique using biotin-labeled rabbit serotonin-antibody.

The distribution of serotonin neuronal somata in the brain of the rabbit was demonstrated by use of immunoperoxidase technique. The serotonin antibody obtained from a rabbit was applied with the two-step ABC (avidin-biotin-peroxidase complex) method after biotinylation of this antibody. The location, shape, size and immunoreactivity of these somata were clarified in our permanent preparations without non-specific and background staining. These perikarya were distributed in the raphe nuclei, linear nuclei and interpeduncular nucleus, within and around the medial lemniscus, in the mesencephalic periaqueductal grey lateral to the raphe dorsalis, and in the reticular formation of the brain stem. However the so-called "masked indolamine cells" were not observed in the hypothalamus or the area postrema, as our experimental animals were not given any pharmacological pretreatment. These results were essentially similar to those from other mammalian species, especially the rat.

Animals↗
PubMed1985

Three functionally distinct helper T-cell clones: the roles for antigen non-specific helper factors in B-cell activation through two different pathways.

We established three functionally distinct purified protein derivative (PPD)-reactive T-cell clones (B11.15, B12.F and D-2). Clone B11.15 could co-operate with DNP-primed B cells to induce anti-DNP IgG plaque-forming cell (PFC) responses only when high amounts of PPD were added to the culture, whereas stimulation of a low amount of DNP-PPD was ineffective (factor-mediated interaction). On the other hand, clone D-2 activated those B cells in a MHC-restricted manner only when DNP-PPD was added to the culture (cognate interaction). B12.F could stimulate B cells with either PPD or DNP-PPD. Antigen non-specific helper factors (lymphokines) responsible for B-cell activation produced by cloned T cells upon stimulation with PPD and antigen-presenting cells were then investigated. Lymphokine activities determined in the present study were IL-2, BCGF I, BCGF II and TRF. BCGF I activity was determined by proliferation-inducing activity on purified B cells in the presence of anti-IgM antibody. BCGF II activity was measured by proliferation-inducing activity on purified B cells in the presence of dextran sulphate. TRF activity was determined on DNP-primed B cells for inducing further differentiation into anti-DNP IgG PFC. BCGF I active molecules were eluted in the fraction at apparent MW of 50,000-70,000 and 8,000-10,000 in gel-permeation column chromatography.

Animals↗
PubMed1985

[Studies on CA 125, CA 19-9, TPA and IAP as tumor markers in patients with ovarian cancer].

CA 125, CA 19-9, TPA and IAP were useful for diagnosis of ovarian cancer. CA 125 was localized immunohistochemically in the surface and cytoplasma of cancer cells of serous cystadenocarcinomas, clear cell carcinomas, endometrioid carcinomas and undifferentiated carcinomas and CA 19-9 localized in mucinous cystadenocarcinomas and endometrioid carcinomas. When the cut off value of CA 125 was set by 35 U/ml in the control group, the patients with serous cystadenocarcinoma showed positive in 81%, resulting by 1,766.5 U/ml as an average value. As for CA 19-9 having set by 37 U/ml as the cut off value, patients suffering from mucinous cystadenocarcinoma who revealed positive by 60%, were 194 U/ml in an average value. It is considered that measuring values of CA 125 and CA 19-9 are valuable not only to diagnose as ovarian cancers but also to evaluate their prognosis.

Adenocarcinoma, Mucinous↗
PubMed1985

[Isolated fourth ventricle simulating the Guillain-Barré's syndrome].

Recently the symptoms caused by isolated dilatation of the fourth ventricle have become the subject of discussion under the name of the Isolated Fourth Ventricle (IFV). IFV is caused by occlusion of the aqueduct of Sylvius as well as the foramen of Luschka and Magendie after shunt procedure for the internal hydrocephalus, although the etiological back-ground is not fully understood yet. Most of the symptoms of hitherto reported cases are due to dysfunction of the lower cranial nerve and cerebellum. We reported an interesting adult case of IFV, whose symptoms were very much simulating the Guillain-Barré's syndrome and were completely relieved with fourth ventricle-peritoneal shunt. Clinical history was analyzed with repeated CT scans and was discussed with review of the literature.

Adult↗