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Biomedical subjects

Y Sakano

Publications and source records attributed to Y Sakano.

At least 55 records · Page 3Linked to original sources
PubMed1990

Effects of reduced malto-oligosaccharides on the thermal stability of pullulanase from Bacillus acidopullulyticus.

We investigated the effects of the reduced malto-oligosaccharides, D-glucitol (G1-OH), maltitol (G2-OH), maltotriitol (G3-OH), maltotetraitol (G4-OH), and maltopentaitol (G5-OH) on the thermal stability of Bacillus acidopullulyticus pullulanase (EC 3.2.1.41). The thermal stability depended on the concentration of D-glucitol; after heat treatment for 90 min at 60 degrees in the presence of 0.56, 0.28, 0.14, or 0M G1-OH, the residual activity was 100, 80, 32, and 10% of the control, respectively. Stability increased with the number of glucosyl residues in the alditols added; the effects of G3-OH, G4-OH, and G5-OH on stability were remarkable. Addition of 30% G2-OH, G3-OH, and G4-OH also contributed to the thermal stability of the pullulanase immobilized onto chitosan beads treated with glutaraldehyde. A high concentration of G2-OH stabilized other debranching amylases, Klebsiella pneumoniae pullulanse, Bacillus sectorramus pullulanase, and Pseudomonas amyloderamosa isoamylase (EC 3.2.1.68) under heat treatment for 48 h at 60 degrees, as well as the pullullanase of B. acidopullulyticus.

Amylases↗
PubMed1990

Nonenzymatic glycation alters properties of collagen as a substratum for cells.

Acid-soluble collagen from rat skin was glycated in vitro by incubating with 0.2 M D-ribose at 37 degrees C for several days, and properties of the glycated collagen as a substratum for fibroblasts 3 Y 1 were studied. The cells attached but spread poorly on the glycated collagen as compared with on the untreated collagen. Thymidine incorporation by the cells on the glycated collagen was higher than that by the cells on untreated collagen. These results indicate that the glycation significantly alters properties of collagen as a substratum for cells.

Animals↗
PubMed1989

Cell-adhesive immunoglobulin M in human plasma.

Human plasma contains a cell-adhesive protein that has a structure related to immunoglobulins. This protein was purified by affinity chromatography on an elastin-Sepharose column and by Mono Q anion-exchange chromatography. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis under non-reducing and reducing conditions revealed that this protein is a kind of immunoglobulin M (IgM). Antibodies against the mu chain and against the Fc region of IgM inhibited the adhesion of cells to this protein. Addition of the peptide GRGDS into media inhibited the adhesion, too. These results suggest that this protein is a special subset of IgM having a cell-binding sequence in the Fc region. We propose the name "cell-adhesive immunoglobulin M (CA-IgM)" for this protein. CA-IgM binds to alpha-elastin and laminin suggesting that it may play a role in the interaction between cells and the extracellular matrix.

Animals↗
PubMed1989

[Relapsing polychondritis: report of 4 cases].

Four cases of Relapsing Polychondritis followed in the Hospital do Servidor Publico Estadual de Sao Paulo, since 1985, are reported and discussed. Relapsing Polychondritis, an auto-immune disease that destroys the pinnal and nasal cartilage has been observed occasionally around the world. Since it was first defined and described, in 1923, about 250 cases have been reported.

Adult↗
PubMed1987

Cross-linking of collagen by ascorbate-copper ion systems.

Cross-linking occurred rapidly in collagen when it was treated with ascorbate-copper ion systems. Sodium dodecyl sulfate-polyacrylamide gel electrophoretic patterns of CNBr peptides suggest that in some respects the modifications by the ascorbate-copper ion systems mimicked those that occurred in collagen with aging.

Animals↗
PubMed1985

Neutral oligosaccharides in the urine of a patient with glycogen storage disease type II.

Neutral oligosaccharides were isolated from urine of an adult patient with glycogen storage disease type II, a deficiency of lysosomal acid alpha-glucosidase, by chromatography on columns of activated charcoal, Dowex 50 X 2 and Dowex 1 X 2. Total neutral oligosaccharides in the urine of the patient were increased about 5-fold as compared with those in normal controls. The most accumulated oligosaccharide was separated by Bio-Gel P-2 column chromatography, and finally purified by paper chromatography. Based on various studies, including carbohydrate analysis, chemical ionization mass spectrometry, fast atom bombardment mass spectrometry, degradation by glucoamylase and isopullulanase, and methylation analysis, the structure of this oligosaccharide was deduced to be Glc alpha 1----6Glc alpha 1----4Glc alpha 1----4Glc. This oligosaccharide appears to be accumulated in urine of the patient with acid alpha-glucosidase deficiency as an end product of the hydrolysis of glycogen.

Carbohydrate Sequence↗
PubMed1985

[Beta-amylase].

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Amylases↗
PubMed1985

[Glucoamylase].

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Glucan 1,4-alpha-Glucosidase↗
PubMed1985

[Pullulanase].

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Enterobacter↗
PubMed1985

[Isoamylase].

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Glycoside Hydrolases↗
PubMed1984

Panoramic observation of the mouse gastric mucosa by superwide-field electron microscopy.

Transitional changes in cell population and ultrastructure in the gastric mucosa can be clearly demonstrated by superwide-field electron microscopy. A few undifferentiated cells as well as immature forms of the surface mucous, mucous neck and parietal cells are present in the lower part of the isthmus or the upper part of the neck. Surface mucous cells and mucous neck cells show maturer morphology with their migration to the mucosal surface or to the deep part of the neck, respectively. Parietal cells may migrate either upwards or downwards. Immature chief cells appear in the uppermost part of the base and mature as they migrate towards the gland bottom. A transition from mucous neck cells to chief and parietal cells is suggested. Collagen fibers and fibroblasts are more numerous in the foveola and isthmus regions than in deeper parts of the mucosa. Smooth muscle fibers are separated from the muscularis mucosae and run perpendicularly to the mucosal surface.

Animals↗
PubMed1984

Histogenesis of the mouse gastric mucosa, with special reference to type and distribution of proliferative cells.

Histogenesis of the mouse gastric mucosa and the distribution of epithelial cells capable of proliferation were studied by light microscopy, autoradiography with 3H-thymidine and electron microscopy. The formation of the gland begins on day 14 of gestation, while morphological signs of epithelial cell differentiation begin on day 15. The cell types include surface mucous, primitive chief and parietal cells during the late prenatal and first 2 weeks of postnatal development. Immature surface mucous cells and undifferentiated cells in the lower part of the foveola and the isthmus, and primitive chief cells throughout the gland become 3H-thymidine labeled. In addition, surface mucous cells in the superficial epithelium are labeled in fetuses and neonates several hours after birth. By 21 days after birth, primitive chief cells are replaced by chief and mucous neck cells. At that time, immature surface mucous and undifferentiated cells in the isthmus, and mucous neck cells in the upper part of the neck are radio-labeled and form the generative cell zone. The mucosa attains its full thickness by 6 weeks of age. Immature parietal cells rarely incorporate 3H-thymidine during their development. It is suggested that some of the parietal cells may be derived from actively proliferating precursors, i.e., primitive chief cells and mucous neck cells in developing and adult animals, respectively.

Age Factors↗