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Biomedical subjects

Y Sakamoto

Publications and source records attributed to Y Sakamoto.

At least 883 records · Page 49Linked to original sources
PubMed1970

Effects of tetraethylammonium chloride on the membrane activity of guinea-pig stomach smooth muscle.

1. The effects of tetraethylammonium (TEA) on the membrane activity of the antral circular muscle of the guinea-pig stomach were investigated with micro-electrode and double sucrose gap methods.2. In a concentration of 1-1.5 x 10(-3) g/ml. (3-5 mM), the membrane potential was not influenced; the membrane resistance measured by inward current pulses remained the same but the rectifying property of the membrane was suppressed.3. TEA (1-1.5 x 10(-3) g/ml.) enhanced the spike amplitude markedly even from fibres which generated graded responses.4. TEA (1-1.5 x 10(-3) g/ml.) did not increase the maximum rate of rise of the spike but decreased the maximum rate of fall of the spike markedly.5. In Na-free (Tris or sucrose) solution, in K-deficient and excess-K solutions, TEA (1-1.5 x 10(-3) g/ml.) suppressed the rectifying property of the membrane and enhanced the spike amplitude.6. Atropine (10(-6) g/ml.) had no effect on the enhancement of the spike amplitude produced by TEA.7. The minimum concentration of Ca ions required for the effect of TEA on the spike amplitude was one fifth of the normal concentration. TEA also enhanced the spike amplitude in Sr-Krebs.8. The possible role of TEA on the membrane activity is considered to be due to suppression of the K conductance when the membrane is depolarized. Alternative possible roles of TEA on the spike amplitude are also discussed.

Animals↗
PubMed1969

Membrane characteristics of the canine papillary muscle fiber.

Passive and active responses to intracellular and extracellular stimulation were studied in the canine papillary muscle. The electrotonic potential produced by extracellular polarization with the partition chamber method fitted the time course and the spatial decay expected from the cable theory (the time constant, 3.3 msec; the space constant, 1.2 mm). Contrariwise, spatial decay of the electrotonic potentials produced by intracellular polarization was very short and did not fit the decay curve expected for a simple cable, although only a small difference of time course in the electrotonic potentials produced by intracellular and extracellular polarizations was observed. A similar time course might result from the fact that when current flow results from intracellular polarization, the input resistance is less dependent on the membrane resistance. The foot of the propagated action potential rose exponentially with a time constant of 1.1 msec and a conduction velocity of 0.68 m/sec. The membrane capacity was calculated from the time constant of the foot potential and the conduction velocity to be 0.76 microF/cm(2). The responses of the papillary muscle membrane to intracellular stimulation differed from those to extracellular stimulation applied with the partition method in the following ways: higher threshold potential, shorter latency for the active response, linearity of the current-voltage relationship, and no reduction in the membrane resistance at the crest of the action potential during current flow.

Action Potentials↗