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Biomedical subjects

Y Sakamoto

Publications and source records attributed to Y Sakamoto.

At least 667 records · Page 37Linked to original sources
PubMed1984

Non-B hepatocellular carcinoma: influence of age, sex, alcohol, family clustering, blood transfusion and chronic liver disease.

In 144 cases of hepatocellular carcinoma (HCC), 166 cases of cirrhosis without HCC and 142 cases of chronic hepatitis, we examined HBsAg, anti-HBs and anti-HBc in sera and compared the following factors between hepatitis B virus marker-negative and -positive patients: age, sex, alcohol consumption, family clustering of liver diseases, and histories of blood transfusion and post-transfusion hepatitis. Results of this study demonstrated several distinct differences in clinical backgrounds between non-B (negative for HBsAg, anti-HBs and anti-HBc) and B (positive for HBsAg) patients with HCC. Non-B patients were significantly older, had a lower frequency of familial tendencies for liver diseases, and more frequently had cancers other than HCC in their families. Some of these differences were also observed between non-B and B patients with cirrhosis and chronic hepatitis. Among patients with chronic hepatitis, the non-B patients had received blood transfusion or had post-transfusion hepatitis more frequently than the B patients. However, this difference was not apparent in patients with liver cirrhosis or HCC, suggesting that progression of non-A, non-B post-transfusion hepatitis to cirrhosis and HCC may not be as frequent as progression to chronic hepatitis.

Age Factors↗
PubMed1984

Local passive transfer of delayed type hypersensitivity to syngeneic testicular cells in mice.

A means for local passive transfer (LPT) of delayed footpad reaction (DFR) against syngeneic testicular cells (TC) was established in mice. Peritoneal exudate (PE) cells from immunized donors pre-treated with cyclophosphamide (Cy) were transferred with syngeneic TC into a hind footpad of a naive mouse. Antigen specific DFR was successfully transferred by PE cells as early as 3-5 days after immunization and as late as 39 days after immunization. DFR was abolished after treatment of PE cells with anti-0 serum, indicating T cell dependence. No footpad reaction can be elicited in male mice immunized with syngeneic TC without Cy pre-treatment. However, PE cells of those mice can passively transfer footpad reaction locally, suggesting the existence of suppressive mechanism in the donors. Although no suppressive mechanism was disclosed at the present, this LPT system seems a potent tool for further analysis of the regulatory mechanisms.

Animals↗
PubMed1984

[Inverted urothelial papilloma and resembling tumors].

Two cases of inverted papilloma, developed on the bladder neck in 36- and 23-year old males, are presented. From the Japanese literature, 40 cases of inverted papillomas have been collected and are reviewed. In addition, a review of the consecutive series of 173 pathological specimens, obtained by bladder biopsy and at the time of bladder surgery over the past 5 years disclosed a resembling lesion in two cases of bladder tumors. An inverted growth of transitional-cell carcinoma was observed in one case. Transitional-cell carcinoma with abundant microcysts was covered by the non-neoplastic transitional-cell epithelium in the other case. In conclusion, the inverted urothelial papilloma is more likely an inverted growth of the neoplastic epithelium with epithelial metaplasia and microcyst formation, much as is observed in so-called chronic proliferative cystitis, than a neoplastic transformation from chronic proliferative cystitis.

Adult↗
PubMed1984

A simple method for the measurement of pineal melatonin with a high performance liquid chromatography--UV detection.

A new technique for determination of pineal melatonin by a high-performance liquid chromatography (HPLC) with UV detection has been developed. The melatonin level in the pineal glands of normal and orchidectomized rats was measured by this method. The pineal melatonin level gradually decreased after orchidectomy. This method provides new technique for the study of melatonin in endocrinological pharmacology.

Animals↗
PubMed1984

Radical surgery with myocutaneous flap for advanced penile carcinoma: case report.

A 55-year-old male with penile epidermoid carcinoma of stage C (T3N3N0) underwent radical surgery. Emasculation with profuse skin removal, including the bilateral groins, and inguinal and pelvic lymphadenectomy were performed. A urethrostomy was created at the perineum, and the skin defect was covered with tensor fascia lata myocutaneous skin flaps. Immediately after recovery from surgery, 3 cycles of chemotherapy with cyclophosphamide, adriamycin and CDDP and radiation (5,000 rad) of the local area were given. The patient is now free from tumor recurrence 12 months after the surgery.

Carcinoma, Squamous Cell↗
PubMed1984

Salivary immunoglobulins and antibody activities in leprosy.

The technics of immunodiffusion and the fluorescent leprosy antibody absorption (FLA-ABS) test were used to determine the levels of immunoglobulins and their antibody activities against Mycobacterium leprae in the serum and the saliva collected from a total of 110 patients with leprosy (50 lepromatous, 24 borderline, and 36 tuberculoid). The average levels of serum IgG, IgM, and IgA were not significantly different among these patients. In saliva, however, IgM was detected in only two cases with lepromatous leprosy and three tuberculoid cases. Salivary IgG and IgA levels and their ratios to those in the sera were not significantly different according to the classification of leprosy. The percentages of positive FLA-ABS tests in the sera and saliva were compared by using fluorescent antibodies specific for IgG, IgM, and IgA, respectively. The results indicated that M. leprae-specific antibodies in the serum were mainly found in IgG and IgM and, less frequently, in IgA. IgG antibodies were found more frequently in lepromatous and borderline patients than in tuberculoid cases. On the other hand, salivary IgA antibodies against M. leprae were found in a significant number of specimens; whereas IgG and IgM antibodies were scarcely found. However, the percentage of positive FLA-ABS tests caused by salivary IgA antibodies was higher in the patients with tuberculoid or borderline leprosy than in those with lepromatous leprosy. A significant number of patients with tuberculoid or borderline leprosy secreted M. leprae-specific IgA antibodies into saliva without detection of circulating IgA antibodies.(ABSTRACT TRUNCATED AT 250 WORDS)

Adult↗
PubMed1984

[Meningeal gliomatosis models as a chemosensitivity assay system].

Experimental models of meningeal gliomatosis (MG) have been produced by intracisternal inoculation of C6 and 9L glioma cells into Wistar and Fisher 344 rats, respectively. Chemotherapy of these models and in vitro chemosensitivity assay for these cell lines were studied with ACNU, BCNU and VM-26. In vitro chemosensitivity assay revealed that 9L cells were sensitive to all of the anticancer drugs above, and that C6 cells were resistant to ACNU and BCNU, but not to VM-26. In vivo experiment, the survival time of the rats inoculated with 9L glioma cells (9LMG) was prolonged by both ACNU and BCNU but not by VM-26. None of these drugs were effective against the rats inoculated with C6 glioma cells (C6MG). It is concluded that the result of in vitro chemosensitivity assay is not always correlative with that of in vivo. This implies that an in vivo chemosensitivity assay system including MG models is indispensable in researching into chemotherapy of brain tumor.

Animals↗
PubMed1984

[Enhanced effect of reserpine on growth-inhibitory action of ACNU on ACNU resistance C6 glioma].

Reserpine was found to enhance the effect of ACNU on ACNU-resistant C6 glioma (C6/ACNU). When reserpine was added to the culture medium at the concentration of 10 microM, the IC50 of ACNU for C6/ACNU was decreased to the level of that for C6. Intracellular uptake of ACNU increased in both resistant and sensitive cells when 10 microM reserpine was added to the culture medium. This phenomenon is more remarkable in C6/ACNU than in C6.

Animals↗
PubMed1984

Solid-phase radioimmunoassay for the measurement of IgG antibodies specific for the house dust mite, Dermatophagoides farinae.

The sera from 65 asthmatic patients were studied for the measurement of IgG antibodies specific to the house dust mite, Dermatophagoides farinae (D. farinae) by solid-phase radioimmunoassay using polystyrene tubes coated with the antigen extract. The solid-phase radioimmunoassay had about the same sensitivity as the conventional double antibody antigen-binding assay in the detection of mite-specific IgG antibodies. The mean value of IgG antibodies was 26.1 (+/- 39.8) micrograms/ml in patients hyposensitized with D. farinae, 23.9 (+/- 29.3) micrograms/ml in those hyposensitized with house dust (HD), and 21.6 (+/- 35.6) micrograms/ml in non-treated patients. A significant difference was detected between HD-treated patients and normals (p less than 0.05). The levels of IgG antibody tended to increase with the increment of the maintenance dose of the D. farinae or HD used in immunotherapy. In addition, eight patients were evaluated for their IgG antibody levels before and after immunotherapy. In five of them, IgG antibodies increased about two to threefold above the value before immunotherapy. These results suggest that the measurement of IgG antibodies by solid-phase radioimmunoassay may be clinically useful in evaluating the effectiveness of immunotherapy.

Antibodies, Anti-Idiotypic↗