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Biomedical subjects

Y Sadamoto

Publications and source records attributed to Y Sadamoto.

24 records · Page 2Linked to original sources
PubMed1990

[Three-dimensional culture of plucked human hair follicles inside the collagen gel matrix].

Plucked human hair follicles were cultured in collagen gel matrix. Epithelial cells, possibly outer root sheath keratinocytes, appeared from the outer root sheath 4 to 5 days after culturing and continually grew into the gel to form spike-like structures for next 3 weeks. The number and size of the spikes differed in each follicle. Autoradiographically, many DNA-synthesizing cells were seen at the outer cell layer in the enlarged outer root sheath and at the edges in the newly formed spike-like structures. The culture method described here appears to be suitable to study the three-dimensional growth, morphogenesis and differentiation of the outer root sheath cells in vitro.

Adult↗
PubMed1990

[Difference between the hair follicle cell and epidermal keratinocyte in vitro].

We cultured human hair follicle cells (HFC) and epidermal keratinocytes (EK) using mixed collagen membranes as the substrate for the culture, and studied the degree of differentiation of these cells. Morphologically, polygonal epithelioid arrangements were seen in both cultures. However, in the culture of EK compared with HFC, large denucleated cells appeared within shorter periods of culture and stratification of cells were more prominent. In the culture of HFC, keratohyalin granules were not observed and cornified envelope formation was suppressed compared with that in EK. Then it is suggested that HFC and EK are different in the degree of keratinization and of other nature.

Adult↗
PubMed1990

[The effect of 1, 25-dihydroxyvitamin D3 on cultured human hair follicle cells from a patients with vitamin D-dependent rickets type II with alopecia].

We examined the effects of 1, 25-dihydroxyvitamin D3 on the growth and differentiation of cultured human hair follicle cells from a patient with vitamin D-dependent rickets type II [DDR-II] with alopecia. In the presence of 100 nM 1, 25-dihydroxyvitamin D3, the clonal growth of normal hair follicle cells was inhibited and terminal differentiation was markedly stimulated. However, the cells from a patient with DDR-II with alopecia did not respond to the hormone, suggesting a lack of the specific receptors in the cells. The difference in the cellular response to the hormone between the normal hair follicle cells and those from a patient were clear and easily distinguished, and these studies may provide a basis for the rapid diagnosis of DDR-II. Large number of hairs were difficult to obtain from patients with alopecia, and we developed a new culture method to accomplish these studies. Our system may be useful in the culture of HFC from only a few hair follicles.

Alopecia↗
PubMed1989

Culture of dispersed hair follicle cells from plucked out hairs without a feeder layer.

Dispersed hair follicle cells from plucked out hairs have been successfully grown on collagen type IV without a biological feeder layer. The optimal calcium concentration for the culture of these cells was also studied and decided to be 0.3-0.5 mM. In this calcium range and on collagen type IV, colony formation and colony growth were best and big colonies with a paving stone-like cell arrangement were formed. The availability of such cultured cells for the treatment of missing skin is proposed instead of cultured epidermal keratinocytes which have been commonly used for wound dressing.

Calcium↗
PubMed1987

[Advanced gastric cancer with DIC and multiple bone metastasis treated with surgical resection and chemotherapy].

Endoscopic examination of a 35-year-old patient complaining of tarry stool, palpitation and lumbago led to a diagnosis of gastric cancer of Borrmann type 4. Laboratory data and bone scintigraphy revealed findings of DIC and multiple bone metastasis. He was treated with continuous intravenous infusion of FOY, but laboratory data with DIC went from bad to worse. It was considered that resection of the tumor was effective for DIC, then total gastrectomy and partial transverse colonectomy were performed, and the patient recovered from DIC. For the multiple bone metastasis, he received chemotherapy using cisplatinum and mitomycin C, and subsequent bone scintigraphy showed a dramatic improvement. Doxorubicin and 5-fluorouracil controlled peritoneal dissemination of the cancer.

Adenocarcinoma↗