Search PubMed⌕ Search

Biomedical subjects

Y Q Tang

Publications and source records attributed to Y Q Tang.

28 records · Page 2Linked to original sources

Isolation and structure of ranamargarin, a new tachykinin from the skin of Chinese frog Rana margaratae.

A new tetradecapeptide, ranamargarin, has been isolated by Sep-Pak C18 and HPLC from methanol extracts of the skin of the Chinese frog Rana margaratae. The sequence of the peptide is: Asp-Asp-Ala-Ser-Asp-Arg-Ala-Lys-Lys-Phe-Tyr-Gly-Leu-Met-NH2. This structure has been confirmed by synthesis. The peptide is the largest among the amphibian tachykinins and its N-terminal amino acids are quite different from those of the other tachykinins. The formation of the sulfoxide and peak-splitting of ranamargarin during purification procedures are briefly discussed.

Amino Acid Sequence↗

Crystal structure determination of mung bean trypsin inhibitor Lys fragment-bovine trypsin complex--molecular replacement, electron density map at 3.0 angstron resolution.

The orientation and position of the trypsin molecule in the complex crystal cell mung bean trypsin inhibitor Lys fragment (MBILF)-bovine trypsin (BTRY) have been successfully determined by molecular replacement method with the model of the refined bovine trypsin molecule. Starting from the BTRY coordinates which were oriented and located in the correct azimuth and position in the complex cell according to the result from rotation function and translation function, sim-weighted Fourier map with coefficients 2/Fo/-/Fc/ at 3.0 A resolution was calculated. Besides the electron density which is obviously attributed to itself, in the vicinity of the active site of BTRY the dense contour levels corresponding to the MBILF and and its boundary could be clearly seen in this map. The size of MBILF was approximately estimated at 15 x 15 x 25 A.

Crystallography↗

Dermorphin-like immunoreactivity in guinea pig and rat stomach.

Dermorphin is an opioid peptide containing one D-amino acid isolated from amphibian skin. We examined the presence of dermorphin-like immunoreactivity (DMP-IR) in mammalian brain and tissues using an antiserum developed in our own laboratory which works at a dilution of 1:120,000 for Radioimmunoassay (RIA) and can detect 2.1 pg of dermorphin. We were unable to find DMP-IR in guinea pig, rat and toad brain and rat and toad spinal cord either extracted with methanol or with HCl. The guinea pig and rat stomach contained significant amounts of DMP-IR (228.9 pg/mg protein and 97.5 pg/mg protein respectively) when extracted with HCl but not with methanol. The DMP-IR we detected in the stomach is not dermorphin per se. A single peak of DMP-IR was found on Sephadex G-25 gel filtration. When re-chromatographed on reverse phase HPLC, this peak of DMP-IR gave rise to three peaks of DMP-IR.

Animals↗

The study of spectra and X-ray crystal structure of a novel bionics insecticide--(CH3)2NHCH(CH2S2O3)2Na X H2O.

IR and NMR spectra of novel bionics insecticide--C5NS4O6H12Na X H2O have been studied. The molecular and crystal structures of the compound also have been determined. The compound crystallizes in the monoclinic space group C5(2)h-P21/n with a = 8.0972(9)A, b = 16.262(4)A, c = 10.370(3)A, beta = 94.26(2)degrees and z = 4. The result shows that N atom in this compound captures a proton to form HN+ group, Na+ is in statistical disorder. Therefore, the structural formula of the compound is (CH3)2HN+-CH(CH2S2O3-)2 X 1/2(Na+)2 X H2O.

Bionics↗

Preliminary crystallographic studies on bacterioferritin from Azotobacter vinelandii.

Bacterioferritin-cytochrome from Azotobacter vinelandii is an unusual protein containing haem groups as well as iron core like other ferritin. This paper reports the purification of bacterioferritin by affinity chromatography and the formation of brick-red crystals from a solution containing MgCl2. The crystals are optical isotropic with maximum dimensions of 0.4 X 0.4 X 0.1 mm3. The preliminary X-ray crystallographic studies have been performed. 1.5 degrees unscreened precession photographs show that the crystals of bacterioferritin belong to the cubic system, space group I432, with cell dimension 230 A. There are probably 8 molecules in one cubic unit cell and the molecule might have 32 symmetry. A molecular diameter of 115 A is derived from the packing of the molecules and a molecular weight of 826,000 is estimated for bacterioferritin.

Azotobacter↗

Percentage decline in masters superathlete track and field performance with aging.

Masters athletic records in track and field events, published in September 1999, were analyzed to evaluate the percentage decline in maximum physiological performance with increasing age. Records were normalized using the 30s age records as the baseline and studied through to the 90s age range. Track running records declined with age in a curvilinear fashion [y = 1-exp((T-T0)/tau)] whereas the walking and field events declined in a linear manner [y = alpha (T-T'0)]. There were significant differences in the rates of percentage decline in the running events over various distances for both males and females, and significant differences between males and females. Decline with aging was greater for females, and for the longer or endurance running events. There were no differences in the rates of declining function for any of the walking events, and the only jumping event to show a significant difference was the high jump performance, which showed the slowest decline. The walking events declined more slowly than the running events, which declined more slowly than the jumping events. Because of the changes in the weights, heights, and distances at different ages, for both males and females, it was not possible to directly compare rates of decline in the various throwing and hurdling events. The strength-dependent throwing events and the pole vault showed the greatest rates of decline with age. In general terms, men's performance declined to 75% of peak performance in sprint events by the early 70s, in the longer track distances by mid to late 60s, and in field events by mid to late 50s. Women's performance declined to a similar extent by their mid 60s in track events, and by late 40s to early 50s in field events. Some of these differences may be due to differences in training effort and/or other competitive aspects, such as the numbers of athletes involved in the sport, and such differences may be reduced in the future with a more professional approach to these events.

Adult↗