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Biomedical subjects

Y Q Liu

Publications and source records attributed to Y Q Liu.

At least 55 records · Page 3Linked to original sources
PubMed1995

[Experimental study on the effect of abstinence with herbal preparation composite dong yuan gao].

The effect of herbal preparation "Composite Dong Yuan Gao" (CDYG) on the animal model of drug dependence was studied. After the morphinistic models of white rats and mice were made by ever increasing doses of morphine each time, the animals were divided into large dose CDYG group, small dose CDYG group, sustained morphine group, and control group. The withdrawal symptoms were observed after naloxone was given to the animals intraperitoneally. The results showed that in the CDYG groups withdrawal symptoms of the addicted white rats were alleviated, the number of jumps of the addicted mice was reduced, the scores of the principal symptoms of the animals were decreased, while the body weights of the animals were gained. Comparing with the control group, the difference was significant (P < 0.01). The effects of the CDYG enhanced in the large dose group, which suggests that the CDYG has abstinence effects.

Animals↗
PubMed1995

A natural vaccine candidate strain against cholera.

E1 Tor Vibrio cholerae (EVC) strains may be classified into two kinds-epidemigenic (EEVC) strains and non-epidemigenic (NEEVC) strains-based on a phage-biotyping system. A large number of EEVC strains have been screened for toxigenic and putative colonization attributes. One such naturally occurring strains (designated IEM101) has been found which is devoid of genes encoding cholera toxin (CT), accessory cholera enterotoxin (ACE), zonula occludens toxin (ZOT), but possesses RS1 sequences and toxin-coregulated pilus A gene (icpA) although icpA is poorly expressed. It expresses type B pili but does not possess type C pili. It is an E1 Tor Ogawa strain and does not cause fluid accumulation in rabbit ilcal loop tests. Active immunization of rabbits with strain IEM101 elicited good protection against challenge with virulent strains of V. cholerae O1. Oral administration caused no side effects in 15 human volunteers, colonized the gut for four to ten days and elicited good immune responses.

Animals↗
PubMed1994

Triterpenoid saponins from Madhuca butyracea.

Further investigation of the seeds of Madhuca butyracea yielded two new triterpenoid saponins, namely butyrosides C and D whose structures were established by means of chemical and spectral analyses as 3-O-beta-D-glucuronopyranosyl protobassic acid 28-O-alpha-L-rhamnopyranosyl(1-->3)-beta-D- xylopyranosyl(1-->4)-alpha-L-rhamnopyranosyl(1-->2)-alpha-L-arabinopy ranoside and 3-O-beta-D-glucuronopyranosyl 16 alpha-hydroxy protobassic acid 28-O-beta-D-apiofuranosyl(1-->3)-beta-D-xylopyranosyl(1-->4)-alpha -L- rhamnopyranosyl(1-->2)-alpha-L-arabinopyranoside, respectively.

Carbohydrate Sequence↗
PubMed1994

Neurologic sequelae associated with foscarnet therapy.

OBJECTIVE: To report three cases of possible foscarnet-induced neurologic sequelae. CASE SUMMARY: We report two cases of seizures and one case of hand cramping and finger paresthesia after starting foscarnet therapy with no evidence of predisposing risk factors, such as serum laboratory abnormalities, renal dysfunction, or known central nervous system (CNS) involvement. All three patients had stable laboratory values during therapy and when the neurologic adverse effects occurred. All patients were receiving appropriate dosages of foscarnet. DISCUSSION: The incidence of seizures in AIDS patients was reviewed. A history of CNS lesions, infections, and/or AIDS per se may increase the risk of a neurologic adverse effect while receiving foscarnet therapy. Acute ionized hypocalcemia may cause these neurologic adverse effects. Ionized hypocalcemia is transitory, is related to the rate of foscarnet infusion, and may not be reflected as a change in total serum calcium concentration. CONCLUSIONS: Foscarnet probably contributed to the neurologic adverse effects reported here. Foscarnet may need to be administered at a slower rate than is recommended by the manufacturer. Electrolytes must be monitored closely; however, a neurologic adverse effect may not be foreseen.

Acquired Immunodeficiency Syndrome↗
PubMed1994

Human papillomavirus in condyloma acuminata and other benign lesions of the female genital tract.

PCR was used to detect (HPV) in condyloma acuminata (CA), pseudocondyloma whose cause is unclear, and the other benign lesions in female genital tract. The results showed that HPV prevalence rate in CA was 98.2%, type 6 was in 11 was 85.7%, and that in pseudocondyloma, cervical polyp, cervical erosion and chronic vaginitis was 40.4%, 76.2%, 77.3% and 16.0%, respectively. Even in normal vaginal tissue, there existed HPV subclinical infection. HPV prevalence rate in swabs from patient's underwears and normal vaginal tissues was 73.6% and 54.0% respectively. The investigation of estrogen receptor (ER) in CA tissue carried out by immunohistochemical method found that positive ER was achieved in most of CA, and few of normal vulvar tissues was found. This result indicated that the occurrence of CA is probably related to estrogen.

Condylomata Acuminata↗
PubMed1994

[Identification of fruits of Xanthium sibiricum Patr. and X. mongolicum Kitag].

The protein electrophorogram, tissue separation picture and complete powder characteristic picture of these two kinds of fruit of siberian cocklebur are made for the first time, and TLC chromatograms of different solvent extracts are also presented. All these present a scientific basis for crude drugs selling, breed identification and composition analysis of the fruit of siberian cocklebur.

Chromatography, Thin Layer↗
PubMed1993

Genomic cloning of the rat histamine H1 receptor.

A rat histamine H1 receptor gene which lacked introns was isolated from a rat genomic library using recently cloned bovine histamine H1 receptor cDNA [Yamashita et al., Proc. Natl. Acad. Sci. USA, 88, 11515-11519 (1991)]. The receptor protein deduced from this isolated gene was composed of 486 amino acids and showed characteristic properties of G protein-coupled receptors. At the 5'-flanking region of the receptor gene, we have located potential TATA box sequences and consensus sequences for the glucocorticoid response element and AP-2 element. After being subcloned into a mammalian expression vector, the isolated gene was transfected to C6 glioma cells. These cells showed significant binding toward [3H]mepyramine. The binding was inhibited by H1 antagonists or histamine. The mode of this binding was comparable to the binding of membranes derived from rat tissues toward [3H]mepyramine. Northern blot analysis detected a 3.0 kb nucleotide band for histamine H1 receptor mRNAs from rat brain and small intestine when these mRNAs were hybridized with the isolated rat H1 gene. The present results demonstrate the isolation of the rat histamine H1 receptor gene.

Amino Acid Sequence↗
PubMed1993

Histamine content, synthesis and degradation in nasal mucosa and lung of guinea-pigs treated with toluene diisocyanate (TDI).

We have reported the presence of a histamine synthesizing enzyme, histidine decarboxylase (HDC), and histamine degrading enzymes, histamine N-methyltransferase (HMT) and histaminase (diamine oxidase, DAO) in human nasal mucosa and the histamine content of the mucosa. In this study, we demonstrate the influences of the toluene diisocyanate (TDI) treatment on the histamine content and these enzyme activities in guinea-pigs as an animal model of respiratory hypersensitivity. Application of TDI to the nasal vestibuli induced intense nasal allergy-like and mild asthma-like responses in TDI-sensitized guinea pigs. Increases in the histamine content and HDC and HMT activities were observed in the nasal mucosa and lung of TDI-sensitized guinea pigs. No apparent changes in the histaminase activities were observed in either the nasal mucosa or the lung. These data suggest that the turnover rate of histamine is increased in the nasal mucosa and the lung of guinea pigs with respiratory hypersensitivity.

Amine Oxidase (Copper-Containing)↗
PubMed1993

Didanosine-associated eosinophilia with acute thrombocytopenia.

OBJECTIVE: To report a case of didanosine (ddI)-induced acute thrombocytopenia and to discuss the incidence of this occurrence. CASE SUMMARY: A 42-year-old man was admitted to the hospital on November 21, 1991, with acute thrombocytopenia. The patient had an AIDS index diagnosis of Kaposi's sarcoma (KS) in 1988 and had been taking zidovudine (ZDV) for three years without any adverse effects. On July 26, 1991, he complained of increasing fatigue and weight loss. At that time, the therapy was changed from ZDV to ddI. Over the next four months the drug therapy was altered from ddI to ZDV and ZDV to ddI because of increasing fatigue and gastrointestinal symptomatology; all of the other chronic medications were maintained at the same dosages and frequency. All laboratory work remained stable and no new active processes were found during this period. The patient developed acute thrombocytopenia upon his last change to ddI and all of the chronic drug therapy was stopped. His platelet count returned to his baseline value three weeks after the discontinuation of ddI and reinstitution of his chronic medications. DISCUSSION: A review of the literature showed two documented cases of thrombocytopenia with ddI on a dosage escalation study. CONCLUSIONS: Close patient monitoring is essential while administering ddI.

Acquired Immunodeficiency Syndrome↗
PubMed1993

[Indirect testimony of stress conduction from mandible to skull in maxillofacial trauma: morphological changes of temporomandibular joint disc]

There were cerebral injuries accompanied in maxillofacial high velocity projectile wound.Owing to the special anatomic relationship between skull and facial bones,the stress conduction of bone was an important factor of cerebral injury in maxillofacial wound.In this experiment,the canine mandibular regions were wounded by steel spheres,which weight 1.03g,impacting velocity at 1400m/s,to produce severely maxillofacial firearm wounds.The morphological and pathological changes of temporomandibular joint disc of wounded side were observed with light and electron microscopes.The microscopic injuries of disc,including arrangement disorder of figre,spotty edema and some fibrous dissolution,etc,were found.These results have provided an indirect evidence of injurous mechanism of the cerebral injury accompaning maxillofacial high enery misslle wound.

Journal Article↗
PubMed1993

[Studies on the hypoglycemic effect of Rhodiola sachalinensis A. Bor. polysaccharides].

Rhodiola sachalinensis polysaccharides (RSP) isolated from the root of Rhodiola sachalinensis was demonstrated to decrease the levels of blood sugar, liver glycogen and total blood lipid when injected peritoneally to mice at 50, 100 and 200 mg/kg once or for 7 successive days. In addition, RSP was found to decrease blood sugar and total blood lipid in various experimental hyperglycemias induced by injection of adrenaline, glucose and alloxan in mice. RSP exhibit inhibiting effect on the glycogen enhancement induced by glucose as well as strenghtening effect on the glycogen decomposition induced by adrenaline and alloxan.

Animals↗
PubMed1993

[Promotive action of sodium fluoride on precancerous lesions of hepatocellular carcinoma induced by diethylnitrosamine (DEN) in rats--stereologic study of enzyme histochemistry].

In 10 Wistar male rats, partial hepatectomy was performed and followed by intraperitoneal injection of DEN (30 mg/kg) once and giving drinking water containing 80 ppm sodium fluoride (36 ppm F) for 14 weeks. By stereologic method of enzyme histochemistry, it was observed for the first time that the foci of hepatocytic precancerous enzyme alterations (gamma-GT positive, ATPase negative and G-6-Pase negative) were significantly increased in number and size compared with the negative control group. The results suggest that sodium fluoride promoted the growth of precancerous lesions of the liver induced by DEN in rats, and this has provided some data to the understanding of the relationship between fluorosis and neoplasms.

Animals↗
PubMed1993

[Morphometric analysis on the in vitro invasiveness of mouse lung adenocarcinoma variants with different metastatic potential].

The in vitro invasiveness of LA1, LAD, LA5 variants of mouse lung adenocarcinoma (LA795) with different metastatic potentials were examined. Precultured heart fragment (PHF) was used as the target organ, and spheroid aggregates of LA1, LAD, LA5 cells were prepared by cultivation of cell suspension on a gyratory shaker. The invasion-complex was formed by confronting of the PHF and cell aggregate. The invasion-complex was then examined at different time intervals histologically and morphometrically. The micro-computer-aided image analysis system was employed to measure the total area (TA) of the invasion complex and the tumor tissue area (subarea, SA). The ratio of SA/TA was used in estimating the invasion ability. It was shown that the invasion of LA1, LAD and LA5 was progressive in nature and the invasive capability of these three variants were correlative with their metastatic potentials.

Adenocarcinoma↗
PubMed1992

Re-examination of [3H]mepyramine binding assay for histamine H1 receptor using quinine.

[3H]Mepyramine, a potent antagonist of the histamine H1 receptor, has been widely used as a radioligand binding assay for the H1 receptor. Previously, we purified a mepyramine binding protein (MBP) from rat liver, but found that its partial amino acid sequences were very similar to those of debrisoquine 4-hydroxylase isozymes (P450 db1 and db2), which are members of the superfamily of cytochrome P450. Using cloned histamine H1 receptor cDNA, we found that [3H]mepyramine could bind only the H1 receptor and did not bind MBP in the presence of 10(-5) M quinine, an inhibitor of debrisoquine 4-hydroxylase isozymes. We developed a method to determine the contents of the H1 receptor and MBP separately using [3H]mepyramine and quinine and found that MBP is abundant in certain areas of bovine brain.

Animals↗