Collective flow from the intranuclear cascade model.
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Journal Article↗
Biomedical subjects
Y Pang
Publications and source records attributed to Y Pang.
Detection of two groups of 25.2 MDa Tet M plasmids by polymerase chain reaction of the downstream region.
Forty-four Neisseria gonorrhoeae, 12 N. meningitidis, four Kingella denitrificans and one Eikenella corrodens carrying 25.2 MDa Tet M plasmids were analysed using polymerase chain reaction (PCR) to the downstream region of the incomplete Tet M transposon. From each isolate, one of two different PCR fragments of approximately 700 or 1600 bp were obtained. The two different sized PCR fragments had > or = 90% DNA sequence identity with Ureaplasma urealyticum Tet M downstream sequences. The difference between the large PCR fragment and the smaller PCR fragment was a deletion of over 800 bp in the smaller fragment. Both PCR fragments were found in plasmids isolated from N. gonorrhoeae and K. denitrificans. The smaller PCR fragment was found in N. meningitidis plasmids and the larger PCR fragment was found in the E. corrodens plasmid.
Bacterial Proteins↗
[Clinical application of MRI in ophthalmic field].
PURPOSE: To investigate the application of MRI in ocular diseases and analysis the character of MRI-s parameters in these diseases. METHODS: MRI was applied among 68 patients with neurophthalmic disorders, orbits diseases, and intraocular lensions. RESULTS: The position (location) and feature of the lesions were diagnosed with clinical analysis. CONCLUSION: MRI possesses the special value in the differentiation diagnosis of neurophthalmic disease, orbit disordes and intraocular conditions.
Adolescent↗
[An analysis of causes of recurrence, matastasis and death of retinoblastoma].
PURPOSE: To discuss the relationship between diagnosis, treatment and recurrence, metastasis, death of Rb patients. METHODS: To analyse the data of 46 Rb patients treated in our center from July 1967 to May 1992, who had either recurrence, metastasis or died. RESULTS: The main factors affecting Rb prognosis are: 1 early or late diagnosis: 2 the mortality rate increased with the degree of optic nerve involvement: 3 involvement of orbit, cranial cavity and choroid by Rb; 4 clinically, secondary glaucoma often accompanied by optic nerve involvement. CONCLUSIONS: Delay in diagnosis and treatment will result in Rb recurrence, metastasis and death. Early diagnosis and treatment is very important for survival rate.
Child↗
Single polymerase chain reaction for the detection of tetracycline-resistant determinants Tet K and Tet L.
Oligonucleotide primers were used in polymerase chain reaction assays to detect tetracycline-resistant determinants Tet K and Tet L. Forty-three isolates representing 11 genera carrying Tet K and/or Tet L determinants gave appropriate PCR products. The PCR products hybridized with labelled Tet K or Tet L probes, and differentiated the Tet K from the Tet L PCR products. We confirmed that two Haemophilus aphrophilus carried the Tet K determinant and two Veillonella parvula carried the Tet L determinant. This is the first time that either of these two genes have been found in Gram-negative species. Four vaginal samples, previously positive with the Tet M/O PCR assay, were also positive with the Tet K/L assay. This is the first report of a PCR assay for the detection of Tet K and Tet L determinants in bacteria or clinical specimens.
Animals↗
Tetracycline resistance determinants in Mycobacterium and Streptomyces species.
Two of seven tetracycline-resistant (Tcr) Mycobacterium fortuitum group isolates and six Tcr clinical Streptomyces isolates carried gram-positive Tcr determinants (Tet K and Tet L) and Streptomyces resistance determinants (Otr A, Otr B, and Otr C). This represents the first documentation of the acquisition by mycobacteria of determinants coding for antibiotic resistance and suggests the potential for the spread of antibiotic resistance determinants within mycobacterial species.
Base Sequence↗
[Analysis on clinical epidemiology of acute bacterial pneumonia in children].
Results of quantitative culture of pathogenic bacteria in nasopharyngeal secretions of 154 cases of hospitalized children with bacterial pneumonia demonstrated that the highest culture rates were Haemophilus influenzae (39.0%) and streptococcus pneumoniae (20.8%). Recovery rate of H. influenzae and Str. pneumoniae among children of various age groups demonstrated that the smaller the age, the higher the recovery rate. The recovery rates of these two bacteria were highest in December and in November, respectively.
Child↗
[Clinicopathological analysis of rhabdomyosarcoma of the ocular adenexa].
12 cases including 11 males and 1 female of rhabdomyosarcoma of ocular adenexa were analysed. The average age of the patients was 4.2 years. 9 cases of lesion were located in the orbit, 2 in the eyelid and 1 in conjunctiva. Pathologically 8 cases were embryonal forms, 2 alveolar, 2 polymorphic. Immunohistochemical studies were performed in 8 cases, which showed desmin positive. Myoglobin presented strong or weak positive in 5 cases, suspicious positive in 3 cases. The clinical manifestation, prognosis and treatment were briefly discussed.
Child↗
Two essential regulatory elements in the human interferon gamma promoter confer activation specific expression in T cells.
Like interleukin 2 (IL-2), interferon gamma (IFN-gamma) is an early response gene in T cells and both are prototypical T helper cell type 1 (Th-1) lymphokines. Yet IL-2 and IFN-gamma production are independently regulated, as demonstrated by their differential expression in certain T cell subsets, suggesting that the regulatory elements in these two genes must differ. To explore this possibility, the 5' flank of the human IFN-gamma gene was analyzed. Expression of IFN-gamma promoter-driven beta-galactosidase reporter constructs containing 538 bp of 5' flank was similar to that by constructs driven by the IL-2 promoter in activated Jurkat T cells; expression nearly as great was observed with the construct containing only 108 bp of IFN-gamma 5' flank. These IFN-gamma promoter constructs faithfully mirrored expression of the endogenous gene, in that expression required activation both with ionomycin and PMA, was inhibited by cyclosporin A, and was not observed in U937 or THP-1 cells. The region between -108 and -40 bp in the IFN-gamma promoter was required for promoter function and contained two elements that are conserved across species. Deletion of 10 bp within either element reduced promoter function by 70%, whereas deletions in nonconserved portions of this region had little effect on promoter function. The distal conserved element (-96 to -80 bp) contained a consensus GATA motif and a potential regulatory motif found in the promoter regions of the GM-CSF and macrophage inflammatory protein (MIP) genes. Factors binding to this element, including GATA-3, were found in Jurkat nuclear extracts by electromobility shift assays and two of the three complexes observed were altered in response to activation. One or both of these motifs are present in the 5' flank of multiple, other lymphokine genes, including IL-3, IL-4, IL-5, and GM-CSF, but neither is present in the promoter of the IL-2 gene. The proximal conserved element (-73 to -48 bp) shares homology with the NFIL-2A element in the IL-2 promoter; these elements compete for binding of factors in Jurkat nuclear extracts, although the NFIL-2A element but not the IFN-gamma element binds Oct-1. Factors binding to this element in the IFN-gamma gene were present in extracts from resting and activated Jurkat T cells. However, by in vivo footprinting of intact cells, this element was protected from methylation only with activation.(ABSTRACT TRUNCATED AT 400 WORDS)
Base Sequence↗
The results of treatment in 100 patients with limited-stage small cell lung cancer.
BACKGROUND: In an attempt to improve treatment results of small cell lung cancer (SCLC), combined induction chemotherapy and thoracic and prophylactic brain radiation therapy were administered. METHOD: From December 1980 to December 1987, 112 patients with limited-stage SCLC were treated with combination induction chemotherapy and thoracic or prophylactic brain radiation therapy; 100 were evaluable. RESULTS: The complete response rate was 40%, and complete and partial responses totaled 94%, with a 5-year survival rate of 23%. There was no difference in remission and survival rates between three-drug and four-drug induction chemotherapeutic regimens. Six chemotherapeutic courses produced longer survival than less therapy. Although the occurrence of brain metastases was no different with or without prophylactic brain radiation therapy, the 3-year survival after this procedure was improved. CONCLUSION: Treatment of limited-stage SCLC with induction chemotherapy and thoracic and prophylactic brain radiation therapy produced improved remission and survival rates.
Adult↗
Detection of Tet M and Tet O tetracycline resistance genes by polymerase chain reaction.
Degenerate oligonucleotide primers were used in polymerase chain reaction assays to detect tetracycline resistance genes, Tet M and Tet O. Each of 44 clinical isolates and eight laboratory strains, representing 20 different species carrying either Tet M or Tet O determinants, gave appropriate PCR products with the two primer sets. The PCR products hybridized with radiolabelled Tet M or Tet O probes. The PCR assay was then used to evaluate vaginal swab specimens from women with vaginitis and semen specimens from men with prostatis. Seven of eight vaginal samples and five of eight semen samples exhibited PCR products that hybridized with the radiolabelled probes, suggesting the presence of Tet M and/or Tet O genes. PCR-based detection of Tet M and/or Tet O genes holds promise for evaluation of urogenital specimens.
Bacteria↗
Acute nicotine injections induce c-fos mostly in non-dopaminergic neurons of the midbrain of the rat.
Induction of c-fos gene is an immediate and early response in the cascade of molecular events that ultimately lead to long-term alterations in gene expression in neurons. The psychomotor stimulant and positive reinforcing effects of nicotine have been speculated to be mediated by the dopaminergic neurons of the ventral tegmental area (VTA). To identify the precise subsets of VTA neurons of the rat that mediate the acute nicotinergic effects, the pattern of expression of c-fos gene was mapped using immunocytochemical methods. Acute nicotine injections resulted in prominent Fos-like immunoreactivity (-LI) in the medial terminal nucleus of the accessory optic system, the interpeduncular nucleus, and in the caudal linear subnucleus of VTA. The neurons of other VTA subnuclei, viz., the rostral linear, paranigralis, nucleus parabrachialis pigmentosus, and nucleus interfascicularis or the substantia nigra pars compacta did not contain any cells with Fos-LI. Mecamylamine abolished Fos-LI in most of the VTA neurons. These results suggest that acute nicotine injections induce c-fos expression mostly in non-dopaminergic neurons of the ventral tegmental area of the rat and that nicotine induces c-fos most intensely in the interpeduncular nucleus, the superior colliculus, and several other subnuclei of the accessory optic system.
Animals↗
Antiproton production from heavy ion collisions at 14.6 GeV/c.
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Journal Article↗
[CT scan in 52 cases of retinoblastoma].
The results of CT scan in 52 patients (61 eyes) with retinoblastoma (Rb) managed and confirmed by clinical and pathological examination are reported. The features and value of imaging of CT scan are described and discussed. In this series, 96.72% of cases revealed Rb and 90.16% of the cases with Rb tumor showed evidence of calcification on CT scan. The contrast enhancement of the tumor was slight. 19.67% of the cases with Rb tumor showed retrobulbar extension and invaded intracranium (including 3 cases of intracranical tumor extension and one case of trilateral Rb). We think it is important to perform CT scan in suspected cases of Rb, and in patients with Rb invading the orbit or intracranium as well as in bilateral Rb. Some problems in the diagnosis and differential diagnosis are briefly discussed.
Child↗
Forward protons and nuclear transparency in relativistic heavy-ion interactions.
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Journal Article↗
Interferon-gamma gene expression in human B-cell lines: induction by interleukin-2, protein kinase C activators, and possible effect of hypomethylation on gene regulation.
Human interferon-gamma (IFN-gamma) is an important immunomodulatory protein produced predominantly by T cells and large granular lymphocytes (LGLs). Whereas large amounts of data have been accumulated regarding IFN gamma gene expression in these two cell types, little information about IFN gamma expression in other cell types exists. In this study, we have analyzed the production of IFN gamma by the Epstein-Barr virus (EBV)-positive B-cell line, JLP(c), derived from a patient with Burkitt's lymphoma, and another human B-cell line, PA682BM-1, which was derived from an acquired immunodeficiency syndrome patient. Southern blot analysis indicates the presence of an Ig heavy chain gene rearrangement, but no rearrangement of the T-cell receptor beta chain gene or IFN gamma gene in these B-cell lines. Both cell lines were found to express surface IgD and other B-cell surface markers, thus confirming their B-cell lineage. Analysis for surface Ig, cytoplasmic Ig, and secreted Ig indicates that the two cell lines are in relatively early stages of the B-cell differentiation pathway. We now report that PA682BM-1 can be triggered by the protein kinase C (PKC) activators, phorbol 12-myristate 13-acetate (PMA) and (-)Indolactam-v, to secrete IFN gamma, whereas JLP(c) cells spontaneously produce low levels of IFN gamma that can be enhanced by PKC activators and interleukin-2 (IL-2). After activation of the cell lines with IL-2, (-)Indolactam-v, and PMA, increases in cytoplasmic messenger RNAs (mRNAs) of IFN gamma and the IL-2 receptor chains were also observed. The induction of IFN gamma mRNA and protein by IL-2 was completely blocked by a monoclonal antibody to IL-2 receptor p75 (beta chain), but not by the monoclonal antibody to p55 (alpha chain). Analysis of IFN gamma genomic DNA indicates that the gene is not amplified, but that hypomethylation in the 5' noncoding region of the IFN gamma gene has occurred in the B-cell line from the Burkitt's lymphoma patient that spontaneously produces IFN gamma. This finding suggests that the methylation state of the promoter region may play an important role in the control of IFN gamma gene expression in B cells.
Acquired Immunodeficiency Syndrome↗
Cascade for relativistic nucleus collisions.
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Journal Article↗
Stimulation of thymidine kinase activity in baculovirus infected cells is not due to a virus-coded enzyme.
A polyhedrin-positive recombinant autographa californica nuclear polyhedrosis virus (AcNPV) carrying a herpes simplex virus thymidine kinase gene under the control of the SynXIV promoter, a fusion of synthetic and linker-modified polyhedrin promoters, has been constructed. When this recombinant baculovirus was used to infect a variant of Spodoptera frugiperda cells deficient in thymidine kinase (TK-), a high level of TK activity was detected. These results, in conjunction with the demonstration that AcNPV could replicate in TK- S. frugiperda cells and no TK activity was found throughout infection, imply that the wild type virus-stimulated TK activity observed in S. frugiperda (TK+) cells is not contributed by a virus-coded enzyme.
Animals↗