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Biomedical subjects

Y Ono

Publications and source records attributed to Y Ono.

At least 937 records · Page 52Linked to original sources
PubMed1986

Relationship between chlordane and its metabolites in blood of pest control operators and spraying conditions.

Chlordane has been widely used to protect soil and house foundations against termite infestation. Pest control operators (PCOs) are occupationally exposed to chlordane. The relationship between chlordane and its metabolites in blood of PCOs and spraying conditions were investigated. Chlordane and its metabolites were detected in the blood of some chlordane-exposed PCOs, but not in that of the controls. Trans-nonachlor and the metabolites oxychlordane and heptachlor epoxide were detected in the blood of PCOs. Total concentration of chlordane and its metabolites in blood (trans-nonachlor + oxychlordane + heptachlor epoxide) was less than 5.6 ppb (mean: 0.89 ppb). The concentration of chlordane and its metabolites in blood of chlordane-exposed PCOs was significantly correlated with the number of spraying days and the amount of chlordane sprayed, particularly with a large correlation coefficient (r = 0.81, P less than 0.001) with the spraying days in the three months prior to the medical examination. The concentration of chlordane and its metabolites in blood is considered to be a useful indicator of biological monitoring for chlordane exposed workers (PCOs).

Adult↗
PubMed1986

Coronary arterial lesions of Kawasaki disease: cardiac catheterization findings of 1100 cases.

In our institute, 1100 patients with a history of Kawasaki disease have been catheterized for selective coronary arteriography. Their age at examination ranged from four months to 13 years. Coronary artery lesions (CAL) were found in 262 patients. As far as the type of the CAL was concerned, occlusion was noted in 20 (7.6%), segmental stenosis in 15 (5.7%), localized stenosis in 62 (23.7%), aneurysm in 93 (35.5%), and dilatation in 72 patients (27.5%). In terms of the total number of lesions, there were 23 occlusions, 19 segmental stenoses, 109 localized stenoses, 449 aneurysms and 307 dilatations. The 262 patients with CAL were analyzed according to the interval from the onset to the time of selective coronary arteriography. The incidence of both occlusion and segmental stenosis was lowest in the group who were catheterized shortly after the onset of disease, whereas the prevalence of aneurysm was highest in this group. But the prevalence of dilatation was highest in the group of patients who were catheterized late. A total of 12 patients had to undergo femoral arterial thrombectomy for arterial thrombosis following the catheterization, but no other major complication was experienced.

Biopsy↗
PubMed1986

An Epstein-Barr virus (EBV)-determined nuclear antigen (EBNA5) partly encoded by the transformation-associated Bam WYH region of EBV DNA: preferential expression in lymphoblastoid cell lines.

Four peptides were synthesized on the basis of amino acid sequences deduced from a highly spliced transcript encoded by the Bam W, Y, and H fragments of the Epstein-Barr virus (EBV) genome [Bodescot, M., Chambraud, J. B., Farrell, P. J. & Perricaudet, M. (1984) EMBO J. 3, 1913-1917]. Rabbit antisera against three of the four peptides identified a nuclear polypeptide that varied between 22 and 70 kDa in molecular size. Four of 20 EBV-positive human sera contained antibodies against this polypeptide. Since this is the fifth EBV-determined nuclear antigen (EBNA) discovered in growth-transformed cells, it is designated EBNA5. The antigen was detected in virus nonproducer lines (less than 0.01% EBV early antigen expression) and is thus not dependent on the viral cycle. It was differentially expressed depending on the origin of the lines. All 10 lymphoblastoid cell lines tested expressed EBNA5, but it could not be detected in 10 of 11 EBV-carrying Burkitt lymphoma lines. Infection of tonsillar lymphocytes with the B95-8 strain of EBV induced six EBNA5-specific polypeptides that varied between 41 and 70 kDa in molecular size with regular increments of 6 kDa. This may be due to the fact that the EBNA5 coding sequence includes the Bam W internal repeat. Parallel infection of the EBV-negative Burkitt lymphoma line Ramos with the same viral substrain did not induce detectable levels of EBNA5, nor was this antigen present in permanently EBV-converted Ramos sublines. These findings imply that the expression of the viral genome varies among B cells having different phenotypes.

Antibodies, Viral↗
PubMed1986

trans activation of the latent Epstein-Barr virus (EBV) genome after transfection of the EBV DNA fragment.

Transfection of Epstein-Barr virus (EBV)-nonproducer Raji cells with the BamHI Z fragment of EBV DNA induced antigens that were detected with human antiserum against EBV-specific early antigens. Northern blot analysis of transfected cells revealed that one intense RNA band hybridized with the BamHI H and F fragments but not with the BamHI Z fragment. Cooperation between the BamHI H, F, and BamHI Z regions was also confirmed in baby hamster kidney cells that were cotransfected with both fragments. These results indicate that the transfected BamHI Z fragment of EBV DNA induces a trans-acting factor which activates the gene expression of the BamHI H and F region and that the BamHI Z region possibly plays an important role in the latency of EBV.

Animals↗
PubMed1986

Identification of coding regions for various Epstein-Barr virus-specific antigens by gene transfer and serology.

Baby hamster kidney cells were transfected with BamHI fragments of Epstein-Barr virus (EBV) DNA (B95-8 strain) cloned into the pLTR vector containing retroviral enhancer and promoter sequences. Seventeen fragments (BamHI-A, -B, -C, -D, -E, -G, -K, -L, -M, -O, -P, -Q, -R, -U, -V, -X, and -Z) expressed antigenically distinct EBV-specific products recognized by EBV-immune human sera.

Antigens, Viral↗
PubMed1986

Selective elimination of anti-DNA antibody-producing cells by antiidiotypic antibody conjugated with neocarzinostatin.

A new strategy was shown for the manipulation of autoantibody production in humans. Antiidiotypic antibody to human anti-DNA autoantibody was conjugated with neocarzinostatin (NCS), a cytotoxic agent, by using N-succimidyl 3-(2-pyridyldithio) propionate as a coupling agent. Human B cell clones, which produce anti-DNA autoantibodies, were killed by in vitro treatment with antiidiotype (Id)-NCS conjugates, while clones expressing an Id with irrelevant specificity were unaffected. These results indicate that treatment with anti-Id-NCS conjugates can act as a potent and specific means of generating immunosuppression of autoantibody production. This approach will have a significant advantage in aborting clones that are not effectively suppressed for the autoantibodies by anti-Id antibodies alone, and will result in a potential therapeutic treatment for systemic lupus erythematosus.

Antibiotics, Antineoplastic↗
PubMed1986

Effects of flavonoids on xanthine oxidation as well as on cytochrome c reduction by milk xanthine oxidase.

That flavonoids inhibit xanthine oxidase from cow milk was confirmed by measuring oxygen consumption with an oxygen electrode. In contrast, flavonoids did not inhibit glucose oxidase, another oxygen consuming enzyme. Among the flavonoids tested, quercetin, kaempferol, myricetin, chrysin, quercitrin, and morin were potent inhibitors of xanthine oxidase; their inhibition rates (%) were 80, 70, 69, 62, 59, and 51 at 100 microM (except chrysin at 50 microM), respectively. The xanthine oxidase-inhibiting activities of the flavonoids were not always well correlated with the suppressive activities of the flavonoids on cytochrome c reduction by a xanthine-xanthine oxidase system. The inhibition of xanthine oxidase by quercetin was not affected by cupric ion. The partition rates of the flavonoids between n-butanol and a buffer solution seemed to account for some of the inhibition.

Animals↗
PubMed1986

Transmission of hand-arm vibration to the head.

A "tooth impression" was devised to measure head vibration. The vertical head vibration of four subjects was measured with the tooth impression during exposure of the hand to vertical sinusoidal vibration at acceleration levels of 3.15, 10.0, and 31.5 m/s2 root mean square in the range of 8-200 Hz. While in a standing position, the subjects pulled the vibrating handle upward at a force of 5 kg with the elbow joint stretched during the tests. The measurement was repeated twice. The difference between the two measurements was within 3 dB, a level which suggests that this method has good reproducibility. The vibration transmitted to the head was the greatest in the range of 12.5-16 Hz, and the attenuation was about 20 dB. Head vibration decreased by approximately 15 dB per octave at frequencies above 20 Hz as the frequency increased. When the excitation level was increased by 10 dB, the head vibration increased by about 8 dB.

Adult↗
PubMed1986

[A study of prostatic tissue levels of latamoxef, cefoperazone and cefotaxime].

Although Cephem antibiotics are transferred to the prostatic fluid in relatively low levels, they are clinically effective for bacterial prostatitis. In the present study, the prostatic tissue concentration of Latamoxef (LMOX), Cefoperazone (CPZ) and Cefotaxime (CTX) were determined, and their penetration rates into the prostatic tissue were analyzed. Before the transurethral resection of the prostate (TUR-P), 2 g of LMOX (21 patients), 1 g of CPZ (15 patients) and 2 g of CTX (14 patients) were intravenously administered in a total of 50 patients with benign prostatic hypertrophy at our two Hospitals. The prostatic tissue was taken by TUR-P at 30 minutes and 60 minutes after the injection. The serum concentration and the prostatic tissue concentration of the three antibiotics were determined by the bioassay method. Additionally their serum concentration and the prostatic tissue concentration in the six cases of CTX-injected patients were also determined by high performance liquid chromatography (HPLC). The penetration rate into the prostatic tissue was obtained by the formula; the penetration rate = the prostatic tissue concentration/the serum concentration. The prostatic tissue concentrations determined by the bioassay method were, 36.9 +/- 10.6 micrograms/g at 30 minutes after the injection of 2 g of LMOX and 28.0 +/- 9.0 micrograms/g at 60 minutes, 31.0 +/- 8.3 micrograms/g at 30 minutes after the injection of 1 g of CPZ and 21.1 +/- 10.0 micrograms/g at 60 minutes, and 8.8 +/- 4.1 micrograms/g at 30 minutes after the injection of 2 g of CTX and 4.5 +/- 2.0 micrograms/g at 60 minutes (mean +/- S.D.). The penetration rates determined by the bioassay method were 36.2 +/- 10.9% at 30 minutes after the injection of LMOX and 34.8 +/- 15.3% at 60 minutes, 43.6 +/- 14.4% at 30 minutes after the injection of CPZ and 39.7 +/- 24.1% at 60 minutes, and 11.1 +/- 4.1% at 30 minutes after the injection of CTX and 9.6 +/- 3.8% at 60 minutes (mean +/- S.D.). The penetration rate of CPZ and LMOX were significantly higher than that of CTX (P less than 0.05, P less than 0.01). In 6 of the 14 CTX-injected patients, the serum and prostatic tissue concentrations of CTX and its metabolite, desacetyl-CTX, were also determined by HPLC. The penetration rate of CTX into the prostatic tissue was obtained by the formula; the penetration rate of CTX = the prostatic tissue concentration (CTX + Desacetyl-CTX)/the serum concentration (CTX + Desacetyl-CTX).(ABSTRACT TRUNCATED AT 400 WORDS)

Aged↗
PubMed1986

Correlation between MR signal intensity and histologic findings in neurinomas and meningiomas of the brain.

The signal intensity of magnetic resonance imaging (MRI) was compared with the histologic findings in 20 cases of neurinoma and 18 cases of meningioma. The apparatus was a Hitachi G-10 unit (0.15 T). The pulse sequences were SR (500/32), SE (1200/60), IR (1400/400). The intensity of tumor signal, as compared with that of normal white matter signal, was divided into 9 grades. In neurinomas, the signal intensity appeared markedly low with IR and markedly high with SE sequence in the Antoni B type, while it was slightly low with IR and minimally to markedly high with SE sequence in the Antoni A type, and intermediate between Antoni A type and B type in the mixed type. In meningiomas, it appeared moderately to slightly low with IR and slightly to moderately high with SE sequence in the meningotheliomatous type, minimally low with IR and minimally to slightly high with SE sequence in the fibrous type, and intermediate as compared with the above two in the transitional type. The angiomatous type was characterized by a low intensity on SR and IR images and an iso to high intensity on SE images, while the psammomatous type showed a low intensity with all of the sequences used. We clarified that the signal intensity on MRI varied among different histologic types of neurinoma or meningioma, and correlated differences in the intensity of signals on MRI to that of histologic characteristics, such as size and shape of cells, intercellular material, intracellular biologic polymers, area of membrane surface, blood flow and calcified matter.

Adult↗
PubMed1986

[Non-invasive evaluation of right ventricular pressure in children with heart diseases: quantitative assessment by thallium myocardial imaging].

201-thallium myocardial imaging studies were performed to evaluate systolic pressures in the right ventricle of 194 patients. These patients were classified to four groups. Group A (95 cases) consists of 77 patients with congenital cardiac disease, five patients with primary pulmonary hypertension, and 13 patients with history of MCLS. Congenital cardiac diseases included 30 patients with tetralogy of Fallot, 20 with ventricular septal defect, nine with atrial septal defect, and eight with pulmonary stenosis. Group B (35 cases); preoperative state of transposition of the great arteries. Group C (43 cases); post-operative state of congenital cardiac disease whose pre-operative right ventricular systolic pressures represented more than 70% of the left ventricular systolic pressures. This group included 31 patients with tetralogy of Fallot, seven with ventricular septal defect, four with atrial septal defect and one with patent ductus arteriosus. Group D (21 cases); post-operative state of transposition of the great arteries. Fifteen min after intravenous infusion of 30-50 microCi/kg 201-TlCl, myocardial images were obtained in five projections (anterior, LAO 30 degrees, 45 degrees, 60 degrees, and lateral). The angles were determined to demonstrate clearly the interventricular septum and the ventricular free wall. The images of end-diastolic phase were obtained using the ECG-synchronized gated method in each projection. The region of interest (ROI) was defined as a section or slice by drawing two lines perpendicular to the septum, and the counts of the systemic and pulmonic ventricular free wall (Cs and Cp) were analyzed to evaluate the pressure of the pulmonic ventricle. The pressures of the ventricles were obtained by cardiac catheterization performed concomitantly with the cardiac imaging.(ABSTRACT TRUNCATED AT 250 WORDS)

Age Factors↗