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Biomedical subjects

Y Ohkubo

Publications and source records attributed to Y Ohkubo.

At least 145 records · Page 8Linked to original sources
PubMed1985

In vitro binding of 67Ga to isolated rat liver cells.

In order to understand the mechanism of the accumulation of gallium citrate (Ga-67) in normal liver, an in vitro investigation system was developed using isolated rat liver cells, and various basal factors relating to 67Ga binding to normal rat liver cells were studied. In this study, the values of 67Ga binding to the liver cells increased in parallel with the number of cells; however, binding was hardly affected by higher doses of 67Ga. The binding of 67Ga to the cells was inhibited by the presence of ethylenediaminetetraacetate (EDTA) and citrate. Phosphate and bicarbonate (10(-2) M) slightly inhibited 67Ga binding to the cells. The binding of 67Ga to the cells increased as the pH was decreased. These results suggest that 67Ga binding to normal rat liver cells may occur in 'free' gallium. Moreover, the utilization of isolated rat liver cells is useful for understanding the 67Ga accumulation mechanism in normal liver.

Animals↗
PubMed1985

Mucoepidermoid carcinoma of the hard palate.

A case of mucoepidermoid carcinoma originating in the hard palate was presented. Histological diagnosis of the removed mass at the first operation was pleomorphic adenoma. Tumor recurred at the same site three years later. Subtotal maxillectomy was performed and recurrent tumor was histologically diagnosed as low grade malignant mucoepidermoid carcinoma. A two-year follow-up showed no evidence of recurrence. The low grade malignant mucoepidermoid carcinoma strongly resembles pleomorphic adenoma. The differential diagnosis is important for the better treatment and prognosis.

Adult↗
PubMed1985

Relation between 67Ga uptake and the stage of inflammation induced by turpentine oil in rats.

For the establishment of the experimental system to judge easily the effect of anti-inflammatory drug, 67Ga-citrate was used. The weight of granuloma tissues induced by inflammable agent, turpentine oil, gradually increased and reached a maximum at 6 days after the administration of turpentine oil. Gallium-67 accumulation in the inflammatory lesions showed also a maximum at 6 days after that. Both patterns were closely similar each other. These results showed that the processes and/or stages of inflammation could be indicated by the pattern of 67Ga uptake.

Animals↗
PubMed1985

Developmental changes in tissue distribution of gallium-67 citrate.

The developmental changes in the biodistribution of [67Ga]citrate were studied in rats. The uptake of 67Ga in many tissues changed clearly with age and the fluctuation pattern was characteristic in each tissue. The uptake in heart and bone decreased, but increased in epiphysis with development. In liver, a transient increase in 67Ga accumulation appeared in the course of growth. These changes in 67Ga uptake in tissues were in good agreement with the developmental changes in the levels of nucleotides or protein synthesis that have been found by a large number of studies. These results suggest the existence of a close relationship between 67Ga accumulation and the growth rate of tissues.

Aging↗
PubMed1984

Comparative pharmacokinetics of 67Ga among the different routes of injection.

The pharmacokinetic study of 67Ga-citrate (67Ga) following intravenous (i.v.), subcutaneous (s.c.) and intraperitoneal (i.p.) injection was performed in anesthetized rats using the repeated blood sampling method by cannulation technique into the external jugular vein. The disappearance of 67Ga from the blood following i.v. and s.c. injection was best fit a three-exponential equation. There was no significant difference between the areas under the curves following i.v. and s.c. injection of 67Ga. In the case of i.p. injection, the disappearance of 67Ga from the blood was described by a two-exponential equation. However, the maximum blood radioactivity was very low, and the disappearance rate of 67Ga from the blood was extremely slow compared to the other routes of injection. The conclusion from these results was that s.c. injection was as suitable as i.v. injection, but i.p. injection was not appropriate for the distribution study of 67Ga such as scintigraphy or autoradiography. However, i.p. route may be available for a special experiment which needs the long-time retention of 67Ga in the blood.

Animals↗