The study of the evolution of 168 cases of idiopathic myeloid splenomegaly allowed to point out the prognostic value of the clinical, hematologic, isotopic and radiological parameters. The correlation of these data with the histological type of the osteomedullary lesions at the time of the diagnosis, allows to confirm the long survival of the hyperplastic formes (type I), and the more reserved prognosis in advanced myelofibroses (type II and type III together) without it being possible to bring out a significant difference between the average presumption of survival of type II and type III considered each separately. Indeed, average survival is of 82 months for type I, and 60 months for type II and type III together. The regrouping of these two types seems to be justified and allows to individualize two anatomoclinical forms. One is characterized by hyperplastic marrow with reticulinic fibrosis, usually associated with enlarged spleen and sometimes even with polyglobulia but without radiological bone lesions. The other form is characterized by a marrow of middle importance or frankly hypoplastic, fibrous, with or without osteosclerosis, and shows moderate or marked radiological changes (respectively 16,9 p. 100 of the cases, and, as a rule, pancytopenia.

Adolescent↗

PubMedDate not supplied

[Granulopoiesis: comparison of several methods for studying production and bone marrow cellularity (author's transl)].

Several methods measuring the granulocyte production have been compared in normal subjects, hypoplastic granulopenia, chronic leucopenia and acute leukemia: the granulocyte turnover-rate after injection of in vitro 51Cr labelled autologous granulocytes, the efficiency of 75Se-selenothionine in vivo labelling, the flow-out of the myelocyte compartment after in vitro flash-labelling with 3H-thymidine. The two first methods are correlated and measure the global effective production of granulocytes. The third method is a fractional measure of the bone marrow production and is not correlated with the former ones. The ratio between the granulocyte turnover-rate and the flow-out of the bone marrow proliferative pool is a measure of the bone marrow cellularity. Discrepancies between this measure and the granulocyte turnover-rate has been found only in leukaemic patients.

Autoradiography↗

PubMedDate not supplied

[Editorial: Discrepancies in granulocyte survival time (author's transl)].

Important discrepancies between the renewal rate of mature granulocytes measured by in vitro labelling with DF 32P and with sodium chromate 51Cr are observed. The possible explanations are reviewed: errors made in the drawing of the experimental curve of the radioactivity disappearance; labelling of long-living lymphocytes with chromium; elution of 32P; toxicity of DFP for the tagged cells. If the renewal-rate measured by using 51Cr is the real one, as it seems to be, currently accepted models of granulopoiesis will need reappraisal.

Cell Separation↗

PubMedDate not supplied

[Dosage of serum folates by a radio-assay. Comparison of the results obtained by this method and by microbiological method (author's transl)].

A radio-assay, using a lactoglobulin fraction of milk proteins as a folate binder and 125-I pteroyl glutamate as a competitor, has been studied in 130 cases. The results have been compared to those obtained with microbiological methods. A good correlation has been observed. The radio-assay allows the measurement of folate in the presence of agents inhibiting the bacterial growth (methotrexate, trimethoprime). It can be used for assay in the cerebro-spinal fluid. This method appears to be useful for clinical investigations.

Folic Acid↗

PubMedDate not supplied