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Biomedical subjects

Y Mitsuhashi

Publications and source records attributed to Y Mitsuhashi.

107 records · Page 6Linked to original sources
PubMed1986

[The treatment of psoriasis. Critical study of the use of ACNU (nimustine hydrochloride)].

The present study deals with the results of topical treatment of psoriasis with ACNU (nimustine hydrochloride), a water-soluble derivative of nitrosourea developed in Japan. Solutions at concentrations of 0.1 p. 100, 0.2 p. 100 and 0.4 p. 100 were obtained by dissolving ACNU in water and alcohol. An 0.4 p. 100 ointment was prepared by mixing ACNU with polyethylene glycol. Seventeen patients (12 male and 5 female), ranging in age from 19 to 68 years, were treated. Improvement of the skin lesions was observed in all 10 patients treated with the solution and in 6 of the 7 patients treated with the ointment. Clinical and histological pictures of the 4 main cases are shown with photographs. Compared with other treatments, the ACNU solution and ointment have the same effect as corticosteroid ointments of moderate strength or Goeckerman therapy. Side-effects included papules, redness, pigmentation and irritation. ACNU has been used for the treatment of mycosis fungoides, but we were the first to use this drug in psoriasis. This treatment has some side-effects, but they are less severe than those of other anti-cancer drugs. ACNU is relatively expensive and unsuitable for long term use. Thus, topical applications of ACNU may be of some value in cases where conventional treatments have failed.

Adult↗
PubMed1985

Structural relationships of the three stimulatory factors of RNA polymerase II from Ehrlich ascites tumor cells.

The structural relationships of S-II, S-II', and S-I(b) stimulatory proteins of RNA polymerase II purified from Ehrlich ascites tumor cells were investigated. From analysis of the amino acid compositions and tryptic peptide maps of these proteins labeled with radioiodinated Bolton-Hunter reagent, it was concluded that S-I(b) is a part of S-II located at either the amino- or carboxyl-terminal and that only this region mainly contains radioiodinatable amino acid residues when labeled using 125I. On chymotryptic digestion, S-II was cleaved to 21- and 18-kDa fragments in the presence of DNA. The 21-kDa fragment was found to be sufficient for stimulation of RNA polymerase II. It was suggested that S-II' is formed by phosphorylation of S-II in the domain containing the 18-kDa fragment.

Amino Acids↗
PubMed1985

Separate determination of ethylenediaminetetraacetic acid and its calcium chelate in foods by colorimetry.

A simple colorimetric method is described for the separate determination of ethylenediaminetetra acetic acid (free EDTA) and its calcium chelate (Ca-EDTA) in commercial foods. The underlying principle of the method is that free EDTA is adsorbed by a cation exchange column, but Ca-EDTA is not under weak alkaline conditions. The sample was homogenized with 0,1 n-NaOH and then subjected to equilibrium dialysis against 0,02 n-NaOH at ambient temperature for a definite time (not less than 12 h). An aliquot of the dialysate was measured into a beaker. After the pH of the solution had been adjusted to 8.5 it was applied to a pre-packed cation-exchange column (Amino-form). Free EDTA was adsorbed by this column, whilst Ca-EDTA passed through it without any loss. For assay of Ca-EDTA, the pH of the eluate was adjusted to 2.5, followed by the addition of an excess amount of CuSO4 to convert Ca-EDTA into Cu-EDTA. This solution was then divided into two portions of equal volume. One portion was subjected to the assay of free copper ions, and the other to the assay of total (the sum of free and chelated) copper ions. The difference between the two values is a measure of Ca-EDTA. For the assay of free EDTA, EDTA adsorbed by the column was eluted with 0.5 n-acetate buffer (pH 5.0), followed by the treatment in the same manner as in the case of Ca-EDTA.(ABSTRACT TRUNCATED AT 250 WORDS)

Chromatography, High Pressure Liquid↗
PubMed1983

Genetic counseling for skin diseases. A statistical analysis of 182 cases.

A statistical study was carried out on 182 cases who seeked out genetic counseling about skin diseases. The size of the latent demand for the counseling was assessed to be as much as 3.0% of the outpatients. Most clients were in their twenties. A representative consultand was the patient's child or sibling. The main diseases in question were cleft lip and palate (16%), nevus pigmentosus (12%), Recklinghausen's diseases (8%) and psoriasis (8%). Estimation of the recurrence risk was impossible in 3%, approximate in 54% and accurate in 43%. This defective estimation is due to the insufficiency of the available genetic data, e.g. carrier frequency, penetrance rate, contraction rate by age, etc. The high-risk cases were found to be 36%. In 9 cases (5% of all the counselings) the client decided to avoid reproduction. In 30 cases (16%), the clients asked for counseling after having conceived or given birth to a consultand. They were thought to be too late from the standpoint of prophylaxis of inherited diseases. Our conclusion is that genetic counseling offers some measure of disease prevention and therefore will become an essential branch or dermatology in the future.

Adolescent↗
PubMed1980

[Below-zero habituation of the orienting reflex: study in skin resistance reflex (author's transl)].

Two experiments were designed to investigate a below-zero habituation of the skin resistance reflex (SRR) component of the orienting reflex to auditory stimuli. In both experiments, subjects were given additional ten stimulus-presentations immediately after initial habituation (extended habituation, EH procedure), and they were compared with those without EH procedure. Effects of EH procedure upon rehabituation and SRRs to novel stimuli (Experiment I), and upon SRRs to novel stimuli under an additional control condition (Experiment II), were examined. The EH procedure enhanced SRRs to novel stimuli and had no effects on the spontaneous recovery. These results suggested that habituation would not proceed beyond the zero-level by the EH procedure.

Adolescent↗
PubMedDate not supplied

Amniotic fluid lactoferrin in intrauterine infection.

Lactoferrin (LF) has been found in most biological fluids including amniotic fluid and cervical mucus in pregnant women and is released from neutrophils in response to inflammation. It is an important component of the host defence against microbial infections due to its antimicrobial properties. Premature labour is caused by amniotic infection and high concentrations of inflammatory cytokines in amniotic fluid with infection are well established. In the present study, LF levels of intrauterine infection in amniotic fluid were measured and the biological significance of LF was investigated. The effects of LF on IL-6 production in cultured amnion cells were also investigated. The concentrations of LF and IL-6 in amniotic fluid with chorioamnionitis (CAM) were 8.76+/-0.65 microg/ml and 6.92+/-4.88 ng/ml (n = 28), respectively, and both were significantly higher (P<0.01) than those without CAM (0.86+/-0.81 microg/ml and 0.34+/-0.25 ng/ml; n = 31). LF and IL-6 levels were significantly higher (P<0.01) with CAM. A significant positive correlation between LF and IL-6 levels in amniotic fluid was found (r = 0.91, P<0.01). To our knowledge, this was the first study of its kind, which shows that IL-6 production induced by lipopolysaccharide in cultured cells was significantly inhibited below physiological concentration of LF in the amnion. In addition, the immunohistochemical localization of LF in fetal membranes was investigated. In the fetal membranes with CAM, strong positive staining was observed in amniotic and chorionic membranes, with leucocyte migration, while weak staining was observed in membranes without CAM. These results show conclusively that LF suppresses amniotic IL-6 production under the conditions of intrauterine infection.

Amnion↗
PubMedDate not supplied

Delayed adverse reactions to iodinated contrast media and their risk factors.

PURPOSE: The present prospective survey was performed to obtain information on delayed adverse reactions (DARs) to five types of low-osmolar iodinated contrast media, including their frequency, common manifestations, and the patient's history of allergy. METHODS: We investigated data from 15,890 consecutive patients who underwent contrast-enhanced computed tomography (CT) during a 15-month period. All patients were given a questionnaire asking about the occurrence of DARs, their symptoms and duration, and asked to consult a dermatologist if they had a skin reaction. RESULTS: Of 11,121 patients who returned the questionnaire (response rate, 70.0%), DARs were observed in 1,058 patients (9.5%). DARs tended to occur with higher incidence in patients with no previous history of examinations using contrast media, with past adverse reactions caused by contrast media, with a history of allergy, or with a serum creatinine level greater than 2.0 mg/dl. Among the 331 patients who reported skin reactions, 41 patients consulted a dermatologist. Skin reactions were observed significantly more frequently in patients for whom iotrolan was used, and 60% of these reactions were severe or moderate. CONCLUSIONS: Four risk factors for DARs were identified in the present investigation.

Adolescent↗