Biomedical subjects

Y Mii

Publications and source records attributed to Y Mii.

41 records · Page 3Linked to original sources

PubMed1980

Microvascular anastomosis with absorbable and nonabsorbable sutures--a comparative study in rats.

Polyglycolic acid, polypropylene, and nylon sutures were used to anastomose the aortas of 40 rats. Comparative studies of the healing process at the suture sites were performed. The long-term observations, by means of light and scanning electron microscopy, revealed that the regeneration of endothelium was more rapid and the endothelium had a smoother surface in the aortas that received the polyglycolic acid sutures than in the aortas receiving polypropylene or nylon sutures because the polyglycolic acid sutures were absorbed after 6 weeks. It was concluded that polyglycolic acid suture material may be more desirable than nonabsorbable suture material in microvascular anastomoses.

Anastomosis, Surgical↗

PubMedDate not supplied

Successful arthroscopic treatment of pigmented villonodular synovitis of the knee in a patient with congenital deficiency of plasminogen activator inhibitor-1 and recurrent haemarthrosis.

We report the arthroscopic treatment of pigmented villonodular synovitis (PVNS) in a 13-year-old Japanese boy with congenital partial deficiency of plasminogen activator inhibitor-1 (PAI-1). He was admitted to our hospital with recurrent haemarthrosis of his right knee. Characteristic abnormalities of fibrinolysis included shortened euglobulin lysis time, low PAI-1 activity and low PAI-1 antigen levels. In addition, levels of "active PAI" in the plasma, which is a measure of total PAI bound to exogenous plasminogen activator, were very low. These parameters remained low after venous occlusion. The diagnosis of PVNS was established by synovial membrane biopsy, and arthroscopic synovectomy was performed with adjuvant administration of intravenous tranexamic acid. Subsequent bleeding episodes have been well controlled by oral administration of tranexamic acid on demand.

Adolescent↗

PubMedDate not supplied

Possible involvement of bcl-2 suppression in wild-type p53 gene-dependent cell growth repression in rat osteosarcoma cells.

We recently obtained 3 cloned cell lines demonstrating the p53 mutation from a lung metastatic nodule of a rat transplantable osteosarcoma. In this study, we applied wild-type p53 gene transfer to the rat osteosarcoma cells by lipofection to investigate the effects on cell growth, expression of genes such as waf1/p21, bcl-2, and bax, and nucleosomal DNA fragmentation due to apoptosis. Reconstitution of the p53 gene inhibits cellular growth, and this growth-suppressive effect is partly due to apoptosis involving bcl-2 gene suppression in this tumor type. This rat osteosarcoma model is similar in biologic behavior to human cases and thus is very suitable for further investigation of tumorigenesis and gene therapy for osteosarcoma.

Animals↗

PubMedDate not supplied

Intracytoplasmic crystalloid structures in a malignant fibrous histiocytoma: ultrastructural and cytochemical study.

Crystalloid structures were frequently observed in the cytoplasm of the tumor cells of a malignant fibrous histiocytoma arising in the left leg of a 71-year-old female. These structures were located in the cytoplasm of the fibroblastlike and histiocyte-like tumor cells. The structures consisted of an aggregate of dense granules without unit membrane. Neither glycoproteins nor polysaccharides were detected in these structures on sections stained with chromic acid-phosphotungstic acid or periodic acid-thiocarbohydrazide-silver proteinate. On sections from acrylate-embedded specimens, the structures were easily digested by trypsin and protease but were not sensitive to RNase. Although the significance and origin of these structures remain obscure, the results indicate that the crystalloid structures in the present study are mainly composed of proteinaceous substance.

Aged↗

PubMedDate not supplied

Osteoclast origin of giant cells in giant cell tumors of bone: ultrastructural and cytochemical study of six cases.

To clarify the histogenesis of giant cells appearing in giant cell tumors of bone (GCTB), an ultrastructural and cytochemical study of six cases was performed with both acid phosphatase (ACPase) and tartrate-resistant acid phosphatase (TRACPase) as marker enzymes. TRACPase is considered a specific marker for osteoclasts. ACPase was demonstrated in the macrophagelike stromal cells, the multinucleated giant cells, and the infiltrating macrophages. The enzyme reaction was localized in lysosomal dense bodies and Golgi areas. Intense TRACPase activity was demonstrated in the multinucleated giant cells, whereas a weak reaction was found in the macrophagelike stromal cells. The multinucleated giant cells and macrophagelike stromal cells resembled osteoclasts with regard to the subcellular localization of TRACPase. The present results suggest that the giant cells in GCTB are indeed derived from osteoclasts.

Acid Phosphatase↗