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Biomedical subjects

Y Matsuki

Publications and source records attributed to Y Matsuki.

125 records · Page 7Linked to original sources
PubMed1980

Determination of captopril in biological fluids by gas-liquid chromatography.

The quantitation of captopril in biological fluids by gas-liquid chromatography using a flame photometric detector is described. Captopril was converted into an adduct with N-ethylmaleimide and then into the hexafluoroisopropyl ester. The latter derivative was separated on a 2% OV-210 column and determined by employing the captopril-N-hexylmaleimide adduct as an internal standard. The blood level and urinary excretion of captopril administered intravenously to dogs were measured by the proposed method.

Animals↗
PubMedDate not supplied

Determination of catechol and guaiacol estrogens in urine by capillary gas chromatography/mass spectrometry.

A gas chromatographic (GC)/mass spectrometric method for the simultaneous determination in urine of 2- and 4-hydroxyestrones and hydroxyestradiols, and their monomethyl ethers, is described. Separation of these catechol and guaiacol estrogens was achieved by derivatization into their trimethylsilyl and tert-butyldimethylsilyl ethers, followed by capillary GC on a DB-1 column. The calibration graphs were satisfactorily constructed for these estrogen metabolites by selected ion monitoring at the respective molecular ions using 2-bromoestrone and 4-hydroxyestradiol-d3 3-methyl ether as internal standards. The extraction and purification of the desired estrogens in biological fluids were effected by the combined use of Extrelut-3 and ion exchange columns. The sensitivity and reliability obtained by the newly developed method has proved to be satisfactory for the quantitation of catechol and guaiacol estrogens in human urine.

Estradiol↗
PubMedDate not supplied

Improved methodology for the simultaneous detection of the trichothecene mycotoxins deoxynivalenol and nivalenol in cereals.

A simple and sensitive method has been developed for the analysis of two trichothecene mycotoxins (deoxynivalenol and nivalenol) in cereals. These toxins were extracted with acetonitrile/water (3:1), defatted with n-hexane and purified by a two-step chromatographic procedure using Florisil and Sep-pak columns. The amounts of deoxynivalenol (DON) and nivalenol (NIV) in the column eluates were quantitated by gas chromatography with electron capture detector and gas chromatography-mass spectrometry (selected ion monitoring). The limits of detection of the method were 2.0 micrograms/kg for DON and NIV with recoveries of the toxins spiked into polished rice, wheat and corn at 300 micrograms/kg averaging 87% and 86% respectively.

Chromatography, Gas↗
PubMedDate not supplied

A survey of the occurrence of nivalenol, deoxynivalenol and zearalenone in food stuffs and health foods in Japan.

By adopting a rapid and sensitive method for simultaneous detection of nivalenol (NIV), deoxynivalenol (vomitoxin, DON) and zearalenone (ZEN), the natural occurrence of these mycotoxins in Japan in retail marked cereal flours, popcorn and health foods (totalling 76 samples) was surveyed. Significant contamination by NIV and DON was observed in commercial wheat and barley flours, and partially milled grains which are consumed as rice ingredients. Of particular interest was the presence of DON in popcorn imported from the United States, and the high-level contamination of NIV and ZEN in job's-tears (Hatomugi in Japanese), a widely marketed health food.

Flour↗
PubMedDate not supplied

A limited survey of Fusarium mycotoxins nivalenol, deoxynivalenol and zearalenone in 1984 UK harvested wheat and barley.

A limited survey for the occurrence of nivalenol (NIV), deoxynivalenol (DON) and zearalenone (ZEN) in 1984 UK-grown cereals (31 samples) have been carried out using a new procedure, which is a rapid and sensitive method for Fusarium mycotoxins. NIV, DON and ZEN were detected in 17 (55%), 20 (65%) and 4 (13%) out of 31 samples, and average levels in positive samples were 101 micrograms/kg, 31 micrograms/kg and 1 microgram/kg, respectively. Additional surveys on two wheat and eight barley samples harvested in Scotland have shown that 30%, 60% and 100% of the samples were contaminated with NIV, DON and ZEN, respectively. The contents averaged 391 micrograms/kg of NIV, 39 micrograms/kg of DON and 9 micrograms/kg of ZEN. The results of this survey show that UK-grown cereals were significantly contaminated with NIV, DON and ZEN in a similar way to that observed in Japan, Korea and China. This is the first evidence of the natural occurrence of NIV in UK cereals.

Edible Grain↗