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Biomedical subjects

Y Matsubara

Publications and source records attributed to Y Matsubara.

At least 307 records · Page 17Linked to original sources
PubMed1984

Improved diagnosis of classical vs atypical phenylketonuria by liquid chromatography.

Atypical phenylketonuria among infants with hyperphenylalaninemia must be promptly diagnosed and differentiated from classical phenylketonuria, because these patients require different treatment to prevent irreversible neurological damage. Measurement of pteridines in urine by liquid chromatography has been widely used for this purpose. Here we report a rapid, simplified liquid-chromatographic method for simultaneously measuring urinary biopterin, neopterin, and creatinine. Unlike previous methods, this method involves neither tedious cleanup procedures nor special equipment.

Biopterins↗
PubMed1983

Spontaneous oscillation of electrical potential across organic liquid membranes.

An artificial membrane was studied consisting of an oil layer, nitrobenzene containing picric acid, imposed between two aqueous phases, one of which contained 5 mM hexadecyltrimethylammonium bromide (CTAB) and 5% ethanol. It was found that this system shows rhythmic and sustained oscillation of the electrical potential within the range 150-300 mV with an interval of 2-3 min. In the absence of CTAB or ethanol, no oscillation was observed. It is indicated that in this experiment the concentrations of the solutes are far-from-equilibrium, i.e., the hydrophilic substance, picric acid, was dissolved in the organic phase and the hydrophobic substance, CTAB, was dissolved in the aqueous phase. In addition, the presence of an unstirred layer was suggested to be essential for generating such electrical oscillations.

Animals↗
PubMed1983

[A study on carbohydrate metabolism in surgical patients with special reference to changes of plasma glucagon levels].

The effect of surgery on plasma glucagon levels was studied in forty patients divided into three groups (major, moderate and minor stress groups) according to the severity of surgical stress. In the major and the moderate stress groups, significant increases of plasma glucagon levels were noted during surgery. In these groups, plasma glucagon levels also increased during the first to fourth postoperative days. There seemed to be a correlation between the amount of the increase and the severity of surgical stress. In the minor stress groups, no significant postoperative increase of plasma glucagon levels was noted. On the second postoperative day, the plasma glucagon levels of the moderate stress group were decreased by intravenous administration of several kinds of sugars such as glucose, fructose, xylitol, sorbitol and maltose. The most marked decrease was seen during glucose infusion. And the decreases were continued for two hours after infusion of fructose and maltose, respectively. The results indicate that the severity of surgical stress had a direct effect on the amount of intra- and postoperative increases of plasma glucagon levels. The mechanism of hyperglucagonaemia in surgical patients was also discussed.

Adolescent↗
PubMed1982

Carcinoembryonic antigen producing cultured cell lines enable detection of autoantibodies in sera from patients with gastrointestinal cancer.

Of 16 human malignant tumor cell lines established in our laboratory, seven lines, including three gastric cancer cell lines derived from patients with cancer, were found to carry carcinoembryonic antigen on their cell surface, as determined by radioimmunoassay and indirect immunofluorescence. The existence of antibodies (IgG class) against the gastric cancer cell lines (HPE-GAC-T, -2, -3) and lung cancer cell line (HPL-Ad-K) in the sera of patients with gastrointestinal cancer (incidence 70.8%) was demonstrated by indirect immunofluorescence. In nonmalignant cases and healthy controls, the incidence was 7.7 and 3.2%, respectively. Specificity of the antibodies detected in the sera of patients with gastric cancer was examined by absorption and blocking test methods of immunofluorescence. Even though there was an apparent heterogeneity of the specificity among the antibodies, the detection of such antibodies may be a feasible and practical approach to a clinical diagnosis of malignancy.

Adult↗