Search PubMed⌕ Search

Biomedical subjects

Y Machida

Publications and source records attributed to Y Machida.

At least 307 records · Page 17Linked to original sources
PubMed1975

Synthesis and biological properties of a 9,11-azo-prostanoid: highly active biochemical mimic of prostaglandin endoperoxides.

The 9,11-azo-prostanoid III [(5Z, 9alpha, 11alpha, 13E, 15S)-9, 11-azo-15-hydroxyprosta-5,13-dienoic acid] has been obtained by synthesis and tested for biological activity in systems which are responsive to the prostaglandin endoperoxides PGH2 (I) and PGG2 (II). The azo analog III is a powerful mimic of these endoperoxides with reference to platelet aggregation and release of serotonin when added to human platelet-rich plasma. The analog III is substantially more active (about 7 fold) than PGG2 in stimulating muscle contraction in the isolated rabbit aorta strip. The very great stability of III relative to PGH2 and PGG2 and its potency as a mimic of these important substances suggest that this azo analog will be of considerable value in future studies of the prostaglandin endoperoxides.

Aorta↗
PubMed1973

Complex formation between mycobacterial polysaccharides and fatty acyl-CoA derivatives.

The mycobacterial polysaccharides MMP and MGLP, which contain numerous O-methyl-sugar residues, markedly stimulate fatty acid synthesis catalyzed by a multienzyme complex from Mycobacterium phlei [Ilton, M. et al. (1971) Proc. Nat. Acad. Sci. USA 68, 87-91]. When aqueous solutions containing MMP or MGLP and palmitoyl-CoA were chromatographed on Sephadex G-75 under conditions that widely separate the individual components, polysaccharide and fatty acyl-CoA were eluted in a single peak, indicating formation of a molecular complex. Similarly, the mycobacterial polysaccharides associate with the CoA derivatives of C(18), C(20), and C(22) acids to yield complexes containing maximally 1 mol of fatty acyl-CoA per mol of polysaccharide. The formation of these novel complexes may result from hydrophobic interactions between the paraffin chains of the acyl-CoA derivatives and O-methyl-sugar residues of the polysaccharides.

Chromatography, Gel↗