Treatment with cone moxibustion of chemotherapeutic leukocytopenia in 114 cases.
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Adolescent↗
Biomedical subjects
Y Ma
Publications and source records attributed to Y Ma.
[Effect of Rhodobryum roseum on hemorheology following acute coronary occlusion in dogs].
Following acute occlusion of the left anterior descending coronary artery, the significant and constant hemorheologic changes were observed in venous blood from ischemic area. 30 min after occlusion, the high shear rate (r = 230s-1), middle shear rate (r = 23s-1) and low shear rate (r = 5.75s-1), viscosity of whole blood (eta b) increased significantly, and this change occurred in viscosity of plasma (eta p) and in red cell electrophoretic time (RCET) also. These increases continued thereafter. In another group of dogs, 30 min after coronary occlusion, rapid dripping was performed with Rhodobryum roseum (Huixincao) injection from right femoral vein. After 10 min, eta b at all shear rate reduced significantly, and this reduction was observed in eta p and in RCET also. These findings suggested that the hyperviscosity syndrome developed in acute myocardial ischemic could be blocked by Hui Xin Cao.
Animals↗
[A study on the diagnosis of pulmonary tuberculosis and silicotuberculosis by PCR].
The sputa of 156 patients with lung disease were detected by smear, culture and PCR for mycobacterium tuberculosis. The detectable limitation was 10 fg DNA and 5 organisms. Both sensitivity and specificity of detection by PCR were much better than smear or culture techniques. Our results demonstrated that PCR technique is one of the useful tools in early and rapid diagnosis of pulmonary tuberculosis and silicotuberculosis.
Aged↗
Structure-function relationships of lipoprotein lipase: mutation analysis and mutagenesis of the loop region.
The molecular models of two microbial lipases and human pancreatic lipase (PL) have suggested the existence of common structural motifs including a buried active site shielded by an amphipathic surface loop. In an effort to explore the role of residues comprising the loop of lipoprotein lipase (LPL), we have used site-directed mutagenesis to generate three new LPL variants. In variant LPLM1 we deleted 18 amino acids leaving a loop of only 4 residues which resulted in an LPL protein inactive against triolein substrates. In contrast, two other LPL variants with only partial deletions, involving the apical section of the loop [LPLM2 (-8 amino acids) and LPLM3 (-2 amino acids)] manifested normal lipolytic activity. These findings indicate a critical requirement for the maintenance of charge and periodicity in the proximal and distal segments of the LPL loop in normal catalytic function. This is further highlighted by the detection of a mutation in the proximal section of the loop in a patient with LPL deficiency at position 225 which results in a substitution of threonine for isoleucine. The intact catalytic activity of the partial deletion variants (LPLM2 and LPLM3) further suggests that the apical residues of the loop contribute minimally to the functional motifs of the active site. We support this postulate by showing that the conserved glycine in the apical turn section (G229) can be substituted by glutamine, lysine, proline, or threonine without significantly affecting catalytic activity.
Base Sequence↗
[PAF antagonistic benzofuran neolignans from Piper kadsura].
In a continuing search for PAF antagonists, five benzofuran neolignans have been isolated from the aerial part of Piper kadsura (Choisy) Ohwi, a Chinese traditional drug used for the treatment of inflammation and rheumatic conditions. The structure determination was based upon spectroscopic analysis. Two of the neolignans were found to have new structures and were named as (-)-denudatin B (the enantiomer of denudatin B, II) and kadsurenin M (7S,8S-3,4,3'-trimethoxy-7'-oxo-nor-8',9'-7.O. 4',8,5'-neolignan, V). The known compounds kadsurenon (I), (-)-acuminatin(III) and (+)-licarin A(IV) were also obtained from the same source. (-)-Denudatin B (II) showed potent PAF antagonistic activity in 3H-PAF receptor binding assay.
Benzofurans↗
p53 gene mutations in Chinese human testicular seminoma.
Genomic deoxyribonucleic acid from 17 primary human testicular seminomas was screened for the presence of mutations in exons 5 to 8 of gene p53, using the single strand conformation polymorphism assay, followed by direct deoxyribonucleic acid sequencing. The p53 mutations in 1 allele leading to an amino acid change but a normal (wild-type) sequence in the remaining allele were identified in 4 of 17 seminomas (23.5%). Sites of mutations were in exon 5 (codon 141), exon 7 (codon 238, codon 258) and exon 8 (codon 270). The present study suggested that mutation of the p53 gene is involved in the development of human testicular seminoma as in the case of several other types of human cancers.
Base Sequence↗
[Purification and properties of beta-mannanases from alkalophilic Bacillus N16-5].
Three extracellular alkaline beta-mannanases from an alkalophilic Bacillus N16-5 were purified to electrophoretic homogeneity by (NH4)2SO4 precipitation, DEAE-Sephadex A-25 chromatography, hydroxyapatite chromatography and preparative electrophoresis. Molecular weights and pI values of the beta-mannanases (M-1, M-2 and M-3) were 51000, 38000 and 34700 by SDS-PAGE and 4.3, 2.5 and 2.5 by PAGEIEF, respectively. The optimum pH for enzyme catalysis were 9.0 for M-1 and 10.0 for M-2 and M-3, respectively. All of three enzymes were the most active at 70 degrees C. The activities of three enzymes were strongly inhibited by Ag1+, Hg2+ and Mn2+. Michaelis constants (Km) of the enzyme M-1, M-2 and M-3 for mannase from konjak were 2.9, 1.7 and 12.5 mg/ml, maximum velocities (Vmax) for the saccharide were 27500, 47500 and 15700 mumol.min-1.mg-1, respectively. Konjak was hydrolyzed by the enzyme M-1, and major components in digests were mono-, di-, tri- and tetra-saccharides.
Bacillus↗
[Studies on PAF antagonistic bicyclo(3,2,1) octanoid neolignans from Piper kadsura].
As a continuation of our previous report dealing with the structures of novel neolignans from Piper kadsura (Choisy) Ohwi, two other new bicyclo(3,2,1) octanoid neolignans, named kadsurenin K and kadsurenin L, were isolated. On the basis of spectral analysis and chemical derivatization, their structures were established as 7R,8R,3'R,5'R-delta 8'-3,5'-dimethoxy-4-hydroxy-2',3',4',5'-tetrahydro-2', 4'-oxo-7.3',8.5'-neolignan and 7R, 8R, 3'S, 4'R, 5'R-delta 8'-3,4,5'- trimethoxy-4'-acetoxy-2',3',4',5'-tetrahydro-2'-oxo-7.3', 8.5'-neolignan respectively. Kadsurenin B and kadsurenin C were known compounds. In 3H-PAF receptor binding assay, kadsurenin B, C, K and L demonstrated significant PAF-antagonistic activity.
Animals↗
Soft-x-ray resonant inelastic scattering at the C K edge of diamond.
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Journal Article↗
Developmental expression of brain beta-spectrin isoform messenger RNAs.
We have investigated the expression of brain beta SpIIa and beta SpIb (previously referred to as the beta-subunits of brain spectrin (240/235) and brain spectrin (240/235E), respectively) during mouse brain development. The 9 kb transcript which encodes beta SpIIa is present in fetal mouse brain tissue and increases to a maximal level in a 30-day-old mouse. There is a coordinate accumulation of the 7.8 kb alpha SpIIa mRNA (with beta SpIIa) during mouse brain development. The coordinate expression of alpha SpIIa and beta SpIIa at the mRNA and protein level allows formation of (alpha SpIIa/beta SpIIa)2 tetramers (brain spectrin(240/235)) early in premitotic neuronal development; and avoids turnover of unassembled alpha and beta-subunits. An 11 kb transcript which encodes beta SpIb is not produced in embryonic tissue, and is first seen in a 6-day-old mouse. The protein translation products beta SpIIa and beta SpIb have previously been demonstrated by our laboratory to first appear in fetal mouse brain tissue and at postnatal day 6-8, respectively [J. Neurosci., 7 (1987) 864-874]. The expression of beta SpIb mRNA on postnatal day 6-8, and the appearance of brain spectrin(240/235E) in postmitotic and postmigratory neurons of the cerebellum at this same time; suggests that brain spectrin(240/235E) is involved in differentiated functions of the neuron (formation of cell-cell contacts, formation of dendritic processes and postsynaptic contacts). Thus, the data from the present study demonstrates that the expression of these two neuronal beta-spectrin isoforms is regulated at the level of mRNA expression.
Animals↗
Mapping of the epitope on lipoprotein lipase recognized by a monoclonal antibody (5D2) which inhibits lipase activity.
A monoclonal antibody, 5D2, which inhibits human lipoprotein lipase (hLPL) activity has been widely used for assessment of LPL immunoreactive mass in the clinical evaluation of patients [1] and for analysis of structure-function relationships of LPL [2,3]. We have mapped the epitope on LPL, recognized by the 5D2 antibody, within residues 396-405. Ala400 is the critical amino acid residue conferring epitope specificity. This knowledge confirms that the C-terminal domain of LPL plays a critical role in LPL activity and also provides important information for studies exploring the structure-function relationship of LPL using this antibody.
Amino Acid Sequence↗
Indirect photometric detection of polyamines in biological samples separated by high-performance capillary electrophoresis.
A rapid separation of polyamines and some related amino acids in cultured tumor cells by high-performance capillary zone electrophoresis with indirect photometric detection is demonstrated. 60 cm x 75 microns I.D. fused-silica capillary was used for the separation and quinine sulfate was used as a background electrolyte (BGE). Several polyamines (putrescine, spermidine and spermine), amino acids (lysine, arginine, histidine) and simple cations (K+, Na+) were easily separated in less than 10 min. Using the indirect photometric detection method, femtomole amounts of polyamines extracted from the tumor cells were detected from nanoliter injection volumes, and the signal response was linear over two orders of magnitude.
Animals↗
A missense mutation Pro157 Arg in lipoprotein lipase (LPLNijmegen) resulting in loss of catalytic activity.
Here we report on the molecular defect that leads to a deficiency of lipoprotein lipase (LPL) activity in a proband of Dutch descent. Southern-blot analysis of the LPL gene from the patient did not reveal any major DNA rearrangements. Sequencing of polymerase-chain-reaction-amplified DNA revealed that the proband is a homozygote for G725C, resulting in a substitution of Pro157 for Arg. This substitution alters a restriction site for PvuII, which allowed rapid identification of the mutant allele in family members. Site-directed mutagenesis and transient expression of the mutant LPL in COS cells produced an enzymatically inactive protein, establishing the functional significance of this mutation. This naturally occurring mutation which alters the Pro157 adjacent to Asp156 of the proposed catalytic triad, indicates that this region of the protein is indeed crucial for LPL catalytic activity.
Adult↗
Molecular genetics of human lipoprotein lipase deficiency.
Lipoprotein lipase (LPL) hydrolysis the triglyceride core of circulating chylomicrons and very-low-density lipoprotein, and modulates the levels and lipid composition of low and high density lipoproteins. Worldwide, more than 20 mutations in the LPL gene have been identified in patients with familial LPL deficiency. Most of these mutations are clustered in the region encoded by exons 4, 5 and 6 which forms the proposed catalytic domain of LPL. In French Canadians who have the highest reported frequency for LPL deficiency, three common mutations in the LPL gene have been identified which account for approximately 97% of mutant genes in this group. Simple DNA-based tests for the detection of all these mutations have been developed for the screening for carriers of LPL deficiency. This will facilitate further studies of phenotypic expression in heterozygous carriers and assessment of the risk of atherosclerosis in these individuals.
Arteriosclerosis↗
Radial distribution function in x-ray-absorption fine structure.
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Journal Article↗
Potassium-induced charge redistribution on Si(111) surfaces studied by core-level photoemission spectroscopy.
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Journal Article↗
Developments in the dynamical theory of high energy electron reflection.
High energy electron reflection (HEER) is an important technique in surface science and uses the information carried by high energy electrons reflected from surfaces to study surface structures and surface electronic states. With the development of reflection high energy electron diffraction (RHEED), high energy electron microscopy (REM), and high energy electron energy loss spectroscopy (EEL) in surface science, the usefulness of HEER has been widely recognized and demonstrated. However, a stationary dynamical solution for an arbitrary surface for HEER has not been obtained yet. In this paper, some developments in understanding the dynamical theory of HEER, particularly in recent years, are reviewed: 1. The introduction of the concept of current flow for a semi-infinite crystal model has removed the confusion around the wave points in the "band gap." 2. The consistency between the Bloch wave and multislice in the Bragg case has verified the validity of the argument of current flow and led to the emergence of the BMCR method (Bloch wave + Multislice Combined for Reflection). 3. The failure of the Bloch Wave-Only solution (the BWO solution) on Au (110) surfaces in the Bragg case revealed by the BMCR method implies that previous BWO calculations in the Bragg case might be at fault. 4. The 2-D dependence of the electron wave fields and Picard iteration-like character of multislice calculation in the Bragg case has led to the emergence of an Edge Patching method in Multislice-mode-Only (the EPMO method). The new method yields an infinitely convergent stationary dynamical solution for an arbitrary surface.
Crystallization↗
Phenotypic heterogeneity associated with defective apolipoprotein B-100 and occurrence of the familial hypercholesterolemia phenotype in the absence of an LDL-receptor defect within a Canadian kindred.
Of 163 individuals with a diagnosis of heterozygous familial hypercholesterolemia (FH), only one subject was found to be positive for familial defective apo B-100 (FDB). The eight-member kindred ascertained through this subject who presented with both a clinical phenotype of FH and the FDB apo B-100 (Arg3500----Gln) mutation was studied. Plasma lipid and lipoprotein profiles, apo E phenotypes, apo B gene markers at the 3' hypervariable region and LDL-receptor haplotypes (ApaLI, PvuII, NcoI), were determined, together with LDL-receptor activity on freshly isolated blood lymphocytes. The FDB mutation, present in four relatives, was associated with three different phenotypes: FH and severe hypercholesterolemia, moderate hypercholesterolemia and normolipidemia. The FH phenotype occurred in the absence of any functional LDL-receptor defect. In homozygotes for the absence of the PvuII cutting site who had the apo B mutation, LDL-cholesterol levels were low in the presence of the apo E3/2 phenotype and high in the presence of the apo E4/4 phenotype. None of the major known environmental influences accounted for the wide range of variation in LDL-cholesterol among the affected members. Further observations in the spouse and offspring of the normolipidemic FDB subject confirmed the association of apo E4, the FDB mutation and the PvuII(-/-) genotype with high cholesterol levels. It is concluded that the phenotypic expression of the FDB mutation may vary widely as a function of the genetic environment within a family.(ABSTRACT TRUNCATED AT 250 WORDS)
Adult↗