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Biomedical subjects

Y M Lin

Publications and source records attributed to Y M Lin.

At least 19 recordsLinked to original sources
PubMed1992

Tubal embryo transfer for the treatment of infertility.

The outcome of treatment for male factor infertility with either gamete intrafallopian transfer (GIFT) or in vitro fertilization and embryo transfer (IVF/ET) has been unsatisfactory. A better approach may be tubal embryo transfer (TET). In our medical center, from November 1989 to December 1990, 80 couples (male factor, n = 35, non-male factor, n = 45) entered our program for TET. Superovulation was conducted with either human menopausal gonadotropins (hMG) or gonadotropin-releasing hormone agonist (GnRH-a, buserelin)/hMG. Ovum retrieval (OR) was possible in 73 patients and successful fertilization after insemination occurred in 64 of them. TET was performed only when there was at least one grade III-V embryo. The mean number of embryos transferred was 3.92 +/- 0.13 (range 1-5). There were 35 pregnancies out of 55 TET (64% per TET, 48% per OR). In the group with male factor infertility, OR occurred in 32, and 24 achieved fertilization. Ten pregnancies were achieved after 19 TET (53% per TET, 31% per OR). In comparison, the group with non-male factor infertility had a higher pregnancy rate (69% per TET and 61% per OR). There have been 15 live births, 14 ongoing pregnancies (eight sets of twins and 21 singletons), five abortions and one ectopic pregnancy. Our results indicate that: 1) TET is a valuable treatment for non-tubal factor infertility; and 2) in the group with male factor infertility, it has the advantages of demonstrating fertilization in vitro and preventing unrewarding laparoscopies.

Embryo Transfer↗
PubMed1990

Characterization and peptidase specificity of lugworm (Arenicola cristata) protease C.

1. Lugworm protease C further purified by benzamidine-affinity chromatography, exhibited peptidase specificity for arginyl and lysyl bonds. 2. Protease C consisted of a single polypeptide with a mol. wt of ca 23,000 as determined by SDS polyacrylamide gel electrophoresis, exhibited a u.v. absorption maximum at 280 with an (mg/ml) extinction coefficient of 0.93 and fluorescence spectra typical of a protein containing tryptophan, and had an amino acid composition similar to trypsins. 3. The Kms of the cleavages of the arginyl bond of oxidized insulin B chain and of the lysyl bond of the gly23-ala30 fragment were determined to be 0.72 and 0.96 mM; the corresponding kcats were 38 sec-1 and 1.5 sec-1. The Km and kcat for TAME were 0.042 mM, and 110 sec-1. 4. Lugworm protease C was confirmed to be a trypsin.

Amino Acid Sequence↗
PubMed1990

Plant-derived sweetening agents: saccharide and polyol constituents of some sweet-tasting plants.

Samples of the sweet-tasting species Acanthospermum hispidum DC. (Compositae) (aerial parts), Boscia salicifolia Oliv. (Capparidaceae) (stem bark), Hovenia dulcis Thunb. (Rhamnaceae) (peduncles) and Inga spectabilis Willd. (Leguminosae) (arils) were acquired as part of a continuing search for high-intensity natural sweeteners of plant origin. Following their preliminary safety evaluation, the sweetness of these plants was traced to large amounts of sugars and polyols by taste-guided fractionation, which were identified and quantified using gas chromatography/mass spectrometry. The combined yields of sugars and polyols in the A. hispidum, B. salicifolia, H. dulcis, and I. spectabilis samples investigated were 6.9, 10.1, 18.4 and 12.1% w/w, respectively. These yields are much higher than the total saccharide and polyol content (2.4% w/w) of the sweet dried fruits of Thladiantha grosvenorii (Swingle) C. Jeffrey (Cucurbitaceae), a species which has previously been reported to contain more than 1% w/w of the intensely sweet triterpene, mogroside V. The dried leaves of Symplocos tinctoria (L.) L'Hérit. (Symplocaceae), which were not appreciably sweet, were found to contain only 2.0% w/w of sugars. The results of this investigation, therefore, suggest that unless the saccharide and/or polyol content of a plant part is well over 5% w/w, then it is unlikely to exhibit an overtly sweet taste, unless an intense sweetener is present.

Alcohols↗
PubMed1990

Interferon production from peripheral blood, synovial fluid, and synovial tissue lymphocytes in patients with rheumatoid arthritis and ankylosing spondylitis.

A previous study demonstrated that interferon was present in the serum of 30% of the patients with systemic lupus erythematosus (SLE), which was significantly higher than the 4.5% found in normal controls. We also recently reported that interferon production was deficient from SLE mononuclear cells, which has been attributed to immunodeficiency of the lymphocytes. In this study, interferon measurement included lymphocytes obtained from peripheral blood (PB), synovial fluid (SF) and synovial tissue (ST) in patients with rheumatoid arthritis (RA) and ankylosing spondylitis (AS). PB from normal subjects (NS) was used as a control. The results showed with PHA stimulation, that the interferon level in PBL (L = lymphocyte) in NS (70.0 +/- 67.5) was significantly higher when compared with PBL in RA (27.9 +/- 21.6). However, there was no difference between PBL in NS and AS. With ConA stimulation, the interferon level was significantly higher in the PBL of NS (130 +/- 59) and as compared with the PBL in RA (83.6 +/- 53.5). The SFL in RA (67.8 +/- 31.1) and the STL in RA (77.2 +/- 93.2) were also significantly different. It is concluded that interferon production was deficient not only in PBL in RA, but also in SF and STL in RA. The reduced interferon production from PB, SF and ST lymphocytes in RA patients may be due to previous release or immunodeficiency. Lymphocyte interferon production was normal in AS, which suggests that the lymphocyte abnormality between RA and AS may be different.

Adult↗
PubMed1990

Fasting intragastric total bile acid concentration in patients with gastroduodenal ulcer.

It has been suggested that bile reflux is an important factor in the development of gastric ulcers and even duodenal ulcers. In this study we measured the fasting total bile acid concentration (BAC) in gastric juice and used it to represent the bile reflux. A total of 110 subjects were collected consecutively, including 55 panendoscopically normal subjects as controls, 34 patients with duodenal ulcers (DU), 15 patients with gastric ulcers (GU) and 6 patients with both gastric and duodenal ulcers (GDU). The results showed a wide individual variation of intragastric bile acid concentration (BAC) in normal subjects and nearly 95% of them had concentrations below 160 mumol/L. Normal subjects above 40 years of age had significantly lower BACs than those under 40 years. There was no sex influence on BACs. In the subgroup above 40 years of age, DU and GDU patients had significantly higher BACs than normal controls and GU patients. We conclude that: (1) bile reflux is a common phenomenon in normal subjects, being mostly mild; (2) bile reflux may become milder after 40 years of age; (3) bile reflux is not an important factor in the pathogenesis of gastric ulcers; and (4) duodenal ulcers may not enhance the duodenogastric reflux, except in patients over 40 years of age.

Adolescent↗
PubMed1989

Hinokiflavone, a cytotoxic principle from Rhus succedanea and the cytotoxicity of the related biflavonoids.

Hinokiflavone (1) was isolated as the cytotoxic principle from the drupes of Rhus succedanea L. A comparison of the cytotoxicity of 1 and other related biflavonoids, including amentoflavone (2), robustaflavone (3), agathisflavone (4), rhusflavone (5), rhusflavanone (6) and its hexaacetate (7), succedaneaflavanone (8) and its hexaacetate (9), cupressuflavone (10), neorhusflavanone (11), volkensiflavone (12) and its hexamethyl ether (13), spicataside (14) and its nonaacetate (15), morelloflavone (16) and its heptaacetate (17) and heptamethyl ether (18), GB-1a (19) and its hexamethyl ether (20) and 7"-O-beta-glucoside (21), and GB-2a (22), indicates that an ether linkage between two units of apigenin as seen in 1 is structurally required for significant cytotoxicity. Compounds 13 and 20 also demonstrated significant cytotoxicity.

Antineoplastic Agents, Phytogenic↗
PubMed1989

Changes in per capita and collective dose equivalent due to natural radiation in Taiwan (1950-1983).

This paper deals with the changes in per capita and collective dose equivalent in Taiwan in the past three decades based on the measured terrestrial and cosmic radiation levels and the population distribution as well. Over 70% of the population is concentrated in three population centers which have been naturally formed in the north, middle and south of Taiwan along the west coast as a result of industrialization and population growth. The population has increased 2.5 times in the past 33 y and reached to 1.9 X 10(7) persons, yet the migration of population was from the rural areas where the natural radiation is usually high to the urban areas where the natural radiation is usually low. The resulting collective dose equivalent has been increasing, yet the per capita dose equivalent, on the contrary, has been decreasing.

Background Radiation↗
PubMed1987

Isolation from lugworm (Arenicola cristata) of four proteases that activate cyclic AMP phosphodiesterase.

1. Four cyclic AMP phosphodiesterase-activating activities, designated as A, B, C and D, were isolated from lugworm, Arenicola cristata, by preparative flat-bed isoelectric focusing. Activators C and D were further purified by TSK 3000SW HPLC to homogeneity. 2. Activators A, B, C, and D corresponded to pIs of 4.4, 5.0, 5.2 and 5.4; their mol wts were estimated to be 36,200, 30,500, 30,200 and 28,300 respectively. 3. The protease nature of these activities were confirmed by the inhibition by several trypsin inhibitors of their activation of phosphodiesterase and by their hydrolysis of TAME, a synthetic trypsin substrate. Only protease A also hydrolyzed BTEE, a chymotrypsin substrate.

3',5'-Cyclic-AMP Phosphodiesterases↗
PubMed1982

Calmodulin.

Explore the source record for details and available documents.

3',5'-Cyclic-AMP Phosphodiesterases↗
PubMed1982

[A new human hepatoma cell line: establishment and characterization].

We have grown a human hepatoma cell line, designated as HA22T/VGH, from a 52-yr-old male hepatoma patient since July 1, 1980. This cell line has been subcultured more than 100 passages. The chromosome analysis of HA22T/VGH indicated that the chromosome numbers varied from 70 to 146, with the mode of 73. Methylcellulose soft agar assay showed that approximately 40% of the HA22T/VGH cells formed colonies. The HA22T/VGH produced tumors in nude mice. Histopathological studies of the tumor revealed the arrangement of hepatoma. Detected by the complement fixation method HA22T/VGH cells secreted ceruloplasmin, Factor B, C3, C4, Gc-globulin and alpha 1-acid-glycoprotein. These cells contained the liver associated enzymes: alanine amino transferase, tyrosine amino transferase and gamma-glutamyl transferase. HBsAg and alpha-fetoprotein were not detectable in the HA22T/VGH culture media or cell lysates by the radioimmunoassay.

Animals↗