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Biomedical subjects

Y Lin

Publications and source records attributed to Y Lin.

At least 415 records · Page 23Linked to original sources
PubMed1997

[Clinical values of Pseudomonas corneal ulcer treated by lamellar keratoplasty].

OBJECTIVE: The study was designed to explore the pathological mechanisms and effective methods of treatment for pseudomonas corneal ulcer. METHOD: The nearly perforating pseudomonas corneal ulcer was treated by lamellar keratoplasty and the corneal bottoms were studied histopathologically. RESULTS: 21 cases 21 eyes with pseudomonas corneal ulcers were treated by lamellar keratoplasty, the corneal contour was recovered successfully and a good optical outcome was attained. The study revealed that in the disease not only there was the corneal violent inflammatory reaction, but also there was a large amount of immune cells in the limbal bulbar conjunctiva, sclera, surroundings and base of corneal ulcer. The corneal collagen was degenerative and neovascular vessels invaded the lesion. CONCLUSIONS: Possibly, both the inflammatory and immune reactions exist in the pathological mechanisms of the pseudomonas corneal ulcer. The pathological processes can be stopped by lamellar keratoplasty and a better clinical outcome can be attained.

Adult↗
PubMed1997

A study on plasma norepinephrine and epinephrine levels in TCM liver syndromes.

The levels of plasma norepinephrine (NE) and epinephrine (E) were determined by HPLC in 220 patients with various TCM liver syndromes, and in 96 healthy subjects as controls. The plasma NE and E contents were higher in patients with liver excess syndromes, including Ganqi Yujie (GQYJ [symbol: see text]) syndrome, Ganyang Shangkang (GYSK [symbol: see text]) syndrome, Ganhuo Shangyan (GHSY [symbol: see text]) syndrome, and Ganyang Huafeng (GYHF [symbol: see text]) syndrome; while they were lower in patients with liver deficiency syndromes, including Ganqixu (GQX [symbol: see text]) syndrome, Ganxuexu (GXX [symbol: see text]) syndrome, and Ganyinxu (GYX [symbol: see text]) syndrome. Futhermore, when Ganshen Yinxu (GSYX [symbol: see text]) syndrome was turned into GYSK and then into GYHF, the plasma NE and E contents increased in order of precedence; while when GQYJ was turned into Ganyu Pixu (GYPX [symbol: see text]) syndrome, both NE and E contents decreased. The results suggest that the plasma NE and E contents are reliable objective parameters for the study of pathophysiological basis of the liver excess and liver deficiency syndromes, and the liver-kidney and liver-spleen interrelated syndromes.

Adolescent↗
PubMed1997

[Effects of locally used SD zinc, SD silver and skin transplantation on the combined radiation-burn injury in the rat].

Effects of local use of sulfadiazine (SD) zinc, SD silver in the wound and skin transplantation on the combined injury of 6 Gy radiation and 15% TBSA burn were investigated in the present study. In the three groups of SD zinc or SD silver plus skin grafting at 15 d after combined injury and SD zinc alone, the 15-day survival rates were 63%, 69% and 75% respectively, and significantly higher than that of control group (42%). The repair time of burn wound was shorter and the number of white blood cells was lower than that of control. Animals in the group of local use of SD silver followed by skin grafting on the 7th day all died, but all of the animals survived when the skin grafting was performed after 15 days. These results suggested that SD zinc and SD silver showed some therapeutic effects on the combined radiation-burn injury. In critical phase of the combined injury, surgical operation should be prohibited. However, in the recovery phase, skin transplantation could be performed.

Administration, Topical↗
PubMed1997

[Effect of pathogen-stimulated human CD4+ T cells on gamma delta T cells].

OBJECTIVE: CD4+ helper T lymphocytes and gamma delta T cells play important role in antimicrobial immunity, however, the mechanism of interactions in question still remains to be elucidated. METHODS: CD4+ T cells and gamma delta T cells separated from peripheral blood lymphocytes of healthy donors by panning technique were stimulated with intracellular pathogen-Mycobacterium tuberculosis and human herpes virus-6 as well as extracellular pathogen-Trichomonas Vaginalis respectively in vitro. RESULTS: The stimuli induced CD4+ T cells significantly to proliferate and producing Th1-type cytokine profile-IL-2 and IFN-gamma. With a semipermeable co-culture system in vitro, antigen-activated CD4+ Th1-like cells were found to promote the proliferation and cytotoxicity of gamma delta T cells via soluble factors. CONCLUSION: Functions of gamma delta T cells might be inhanced by CD4+ cells with Th1-type cytokines in antimicrobial immunity.

Antigen-Presenting Cells↗
PubMed1997

[Effects of PGMS on the functions of human platelets and endothelial cells].

OBJECTIVE: To study the effects of Propylene glycol mannte sulfate (PGMS), a heparin-like substance on the functions of platelets and endothelial cells. METHODS: Using 125I labeled monoclonal antibody to measure the expression of GMP-140 on the surface of human platelets; and using naphthol blue black stain method to study the growth of human umbilical vein endothelial cells (HUVEC) and human umbilical vein smooth muscle cells (HUVSMG). RESULTS: PGMS significantly inhibited thrombin stimulated expression of GMP-140 on the surface of human platelets. IC50 was 127 micrograms/ml. Indomethacin also inhibited GMP-140 expression, but its effect was weaker than that of PGMS with the IC50 of 502 micrograms/ml. PGMS did not affect the growth of HUVSMC. On the contrary, it significantly promoted the growth of HUVEC at very low concentration (1.6 micrograms/ml). CONCLUSIONS: PGMS showes anticoagulative effects on both platelets and endothelial cells and it might well be developed as a new antithrombotic and anticoagulateve agent.

Anticoagulants↗
PubMed1997

[Properties of adherence of erythrocytes to endothelial cells in patients with ischemic stroke].

Adherence of erythrocytes to human umbilical vein endothelial cells (HUVEC) in patients with cerebral thrombosis and transient ischemic attack (TIA) were quantitatively studied in the flow chamber system, compared with that in healthy subjects. We found: (a) The adherence of erythrocytes to HUVEC in patients with cerebral thrombosis and TIA increased, compared with that in healthy subjects; (b) The adhesion of erythrocytes to HUVEC in patients with TIA was not significantly different from that of patients with cerebral thrombosis. These suggested that the increased adhesion of erythrocytes to endothelial cells might play an important role in the pathologic process of ischemic stroke.

Aged↗
PubMed1997

[Preliminay studies on polyvinyl alcohol gel and its transderman therapeutic system].

By pouring-cooling method, five kinds of hydrophilic gels (starch, agar, gelatin, CMC-Na and PVA) were prepared. The characteristics of preparation, tenacity and elasticiey were compared. Taking the PVA gel which features better characteristics as the carrier a plaster of verapamine hydrochloride and a traditional Chinese antitussive and expectorant in the form of plaster were prepared. The behavior of drug-releasing in vitro and curative effect were preliminarily studied. The results show that the PVA gel may be a fine carrier for transdermal therapeutic system.

Administration, Cutaneous↗
PubMed1997

[A preliminary report of epikeratophakia for the treatment of Terrien's degeneration].

PURPOSE: To evaluate the efficacy of epikeratophakia for the treatment of severe Terrien's degeneration. METHODS: Four eyes with severe Terrien's corneal degeneration are studied. The lesions were located at the peripheral cornea and the corneal thickness was less then 1/3 of normal. Plano keratoplasty was performed. A lamellar lenticule with a 2 mm scleral rim was grafted on the cornea of which the epithelium had been removed. Keratometry and cycloplegic refraction were performed pre- and post-operatively. RESULTS: Follow-up was between 3 and 13 months (mean, 10 months). All grafts remain clear. The properative mean cylinder was 11.5 diopters and the postoperative mean cylinder was 3.9 diopters. Best corrected visual acuity was improved in 3 eyes. Interface opacification is the main complication, like other forms of epikeratoplasty. Epithelial ingrowth occurred in one eye and the lenticule was replaced. CONCLUSIONS: Epikeratophakia is a simple and effective way for the treatment of severe Terrien's degeneration. This procedure not only increases the corneal thickness, but also hepls the cornea to preserve its transparency, since the Bowman's layer remains intact.

Adult↗
PubMed1997

[Adhesion of erythrocytes of patients with transient ischemic attack to injured endothelial cells].

Adherence of erythrocytes of patients with transient ischemic attack(TIA) to human umbilical vein endothelial cells(HUVEC) treated with H2O2 were quantitatively studied in the flow chamber system. The results showed that injured HUVECs enhanced the adherence of erythrocytes to endothelial cells in patients with TIA. The increased adhesion of erythrocytes to endothelial cells might promote the vascular damage. Consequently endothelium might play an important role in the increased adhesiveness of TIA RBC to endothelial cells.

Aged↗
PubMed1997

[Effect of oxyphenamone, a new inodilator, on isolated cardiac muscles and arteries].

The cardiotonic and vasodilating effects of oxyphenamone were studied in preparations of rat Langendorff's heart, isolated cardiac muscles of guinea pigs and isolated arteries of dogs. In the preparations of rat Langendorff's heart, infusion of oxyphenamone at 0.1 and 1.0 mumol.L-1 increased myocardial contractility and coronary blood flow moderately and decreased heart rate slightly. Oxyphenamone (1-100 mumol.L-1) increased the contractile force of papillary muscle (PM) and left atrium (LA) of guinea pigs dose-dependently. The positive inotropic effect of oxyphenamone (the ED50 was found to be 18.35 mumol.L-1 in PM and 9.17 mumol.L-1 in LA) was close to that of milrinone, an inhibitor of phosphodiesterase(the ED50 was shown to be 6.23 mumol.L-1 in PM and 4.59 mumol.L-1 in LA). The difference between these two agents is that oxyphenamone decreased the spontaneous pulse rate of right atrium, while milrinone increased it. The vasoconstrictions of dog coronary, basilar and mesenteric arteries induced by KCl, 5-HT and CaCl2 were antagonized non-competitively by oxyphenamone at 1 and 50 mumol.L-1, suggesting that oxyphenamone has a relaxant effect on vascular smooth muscles.

Animals↗
PubMed1997

[Study of antisense oligodeoxynucleotide in reversing multidrug resistance and inducing apoptosis of tumor cells].

OBJECTIVE: T o explore an approach to the reversal of multidrug resistance(MDR) of tumor cells. METHODS: Antisense oligodeoxynucleotide (mdr-1-AS PS-ODN) was used. RESULTS: A large amount of DNA fragments was found in mdr-1-AS PS-ODN treated K562/ADM cells and almost all the mdr-1+ K562/ADM cells underwent apoptosis. CONCLUSION: mdr-1-AS PS-ODN could inhibit specifically mdr-1 gene expression, effectively reverse MDR of the tumor cells and promote ADM induced apoptosis of mdr-1+ K562/ADM.

Apoptosis↗
PubMed1997

[Isolation and determination of the seeds of Pachyrrhizus errosus protein by high performance gel filtration chromatography (GFC)].

From the seeds of Pachyrrhizus errosus, three protein constituents, namel PE1, PE2 and PE3, have been isolated and purified by extraction with 5mmol/L phosphate saline (0.9% NaCl) buffer (PB) at pH 7.2, and S-Sepharose Fast Flow Column (2.6cm x 15cm) chromatography which eluted with 5mmol/L phosphate buffer (pH 7.0) containing 1mmol/L NaCl. Three proteins were burther separated on two connected Protein-Pak 60+Protein-Pak 125 [7.5mm x 39cm, 10microm] columns with mobile phase of 0.2mol/L phosphate buffer (pH 6.5). The flow rate was kept constant at 0.8mL/min by YSB-2 type high press pump. The effluent was monitored at a wavelength of 280nm on photodiode array detector. These three proteins are proved to be homogeneous by SDS-PAGE, IEF and HPGFC experiments, and all present the typical absorption spectra in ultraviolet region. The moleculer weights of the three proteins are approxiamtely 33000D, 14500D and 14000D respectively by SDS-PAGE. But as using HPGFC analysis, the MW value of PE2 is 28000D. This indicates PE2 may be composed of two chains joined by disulfide bond, which is further proved from the latter amino acid composition analysis. The isoelectric points of three proteins are 4.5, 6.5 and 7.5 respectively by using IEF. The amion acids compositions of the three proteins were determined with OPA post-column derivatization/fluorescence detection.

Amino Acids↗
PubMed1997

[Rapid spectrochemical qualitative analysis of simple OMA system].

Simple optical multichannel analyzer (OMA) system which is formed by ordinary one dimension CCD camera, spectrograph and microcomputer was introduced in this paper. Spectrochemical analysis feasible of this OMA system was studied. The applied software programed, by us can realize wavelength calibration, spectral line identification and give out automatically the results of qualitative and semidefinite quantity analysis.

English Abstract↗
PubMed1997

Telomerase activity in primary prostate cancer.

PURPOSE: Telomerase activity has been detected in a wide variety of human tumor types. We analyzed the telomerase activity in association with the acquisition of prostate cancer. MATERIALS AND METHODS: Telomerase activity in prostate tissues was examined by PCR-based telomeric repeat amplification protocol (TRAP) assay. RESULTS: Among 31 primary prostate cancers, 28 tissue samples (90%) displayed telomerase activity. The relative level of telomerase activity was associated with the pathological differentiation. High levels of telomerase activity were more frequently detected in poorly differentiated prostate cancer. None of the 10 samples taken from prostates with benign prostatic hyperplasia (BPH) or normal prostates expressed telomerase activity. In another 10 BPH samples obtained from prostate tissue adjacent to cancerous tissue, one of 10 samples (10%) showed weak telomerase activity. Furthermore, we investigated this activity in human prostate cancer cell lines (PC-3, LNCaP, and DU145) and all showed very high activity compared to normal human tissue samples. Four lymph nodes and one bone metastasis also exhibited extremely high telomerase activity. CONCLUSIONS: The present results indicate that telomerase activity might be a marker for detecting malignancy of the prostate and evaluating the malignant potential of prostate cancer.

Adenocarcinoma↗
PubMed1997

p53-mediated cell cycle arrest and apoptosis.

We have generated a series of murine erythroleukemia clones that ectopically express a temperature sensitive mutant p53 allele. In many clones, activation of p53 at low temperature resulted in the accumulation of cells in G1 and in apoptosis. Several cytokines including erythropoietin, IL-3 and the ligand for the Kit receptor blocked p53-dependent apoptosis in p53ts-expressing cells at 32 degrees C. Cytokine-treated cells were reversibly arrested in G1 and resumed growth upon return to 37 degrees C. Certain clones exhibited only a G1 arrest in response to p53 activation at 32 degrees C. One of the these clones secreted erythropoietin and another secreted IL-3. We tested the possibility that autocrine secretion of IL-3 played a role in preventing apoptosis and showed that disruption of the autocrine loop by cell dilution or with neutralizing antibodies to IL-3 restored p53-dependent apoptosis at 32 degrees C. Thus, two properties of p53 protein, namely, its ability to arrest cells in G1 and its ability to promote apoptosis could be uncoupled by cytokines acting as survival factors.

Animals↗
PubMed1997

[Histochemical localization of nitric oxide synthase in the human placenta].

OBJECTIVES: To study localization, distribution and activity of nitric oxide synthase (NOS) in the human placenta and to speculate the action of nitric oxide (NO) during pregnancy. METHODS: NADPH-diaphorasc histochemical method was used to indicate the distribution, localization and activity of NOS in the placentae of normal term pregnancy (n = 21). Severe pregnancy induced hypertension (PIH) (n = 15), intrauterine growth retardation (IUGR) (n = 2) and villi of early pregnancy (n = 16). RESULTS: The NOS reactive product called blue formazan was found in most of syncytiotrophoblast (STr) and located in top of cells in severe PIH and IUGR placentae. In normal term placentae the blue formazan was found in STr and located in base of cells. The number of blue formazan was more than that of PIH and IUGR placentae. Endothelial cells of most capillaries of the terminal villi in severe PIH and IUGR placentae were found to be deposited with blue formazan, but in normal term pregnancy, blue formazan was found only on rare occasion. CONCLUSION: Distribution and localization of NOS in placentae of normal term pregnancy, severe PIH and IUGR cases and early villi are specific. The NOS activity of STr in severe PIH placentae and IUGR placentae are lower than that of normal term placentae. The distribution and localization of NOS within the STr in severe PIH placentae and IUGR placentae are different from those in normal term placentae. The low activity of NOS secreted by placenta may be relative to the pathogenesis of PIH and IUGR.

Adult↗
PubMed1997

Correlation between retinal fluorescein angiography and blood viscosity and other factors in patients with primary open angle glaucoma.

OBJECTIVE: To investigate the correlation among retinal fluorescein angiography, blood viscosity, and other factors in patients with open angle glaucoma (POAG). METHODS: Multiple step regression analysis was made to investigate the correlation between each of the following blood vessel filling times: the arm-choroid (A-CT), arm-retinal artery (A-AT), and retinal artery-venous (A-VT) of the fundus fluorescein angiography (FFA) in 122 eyes with POAG and each of the following related factors in hemorrheology: whole blood apparent viscosity at low, medium and high shear rates, plasma viscosity and hematocrit. Also, the same analysis was applied to investigate the correlation between each of the A-AT, A-VT of the FFA in 70 eyes with POAG and the following factors: systolic blood pressure, diastolic blood pressure, age and whole blood apparent viscosity at low shear rate. RESULTS: The whole blood apparent viscosity at low shear rate was closely related to A-CT and A-AT, while hematocrit was closely related to A-VT of the FFA. The whole blood apparent viscosity at low shear rate and age, especially the whole blood apparent viscosity, was closely related to A-AT, A-VT of the FFA. CONCLUSION: Blood viscosity can affect the filling times of the FFA in POAG.

Blood Viscosity↗