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Biomedical subjects

Y Le Bell

Publications and source records attributed to Y Le Bell.

At least 19 recordsLinked to original sources

Effect of repeated sampling and prestimulation on saliva buffer capacity and flow rate values in children.

Great variations reported in the frequency distribution of buffer capacity values may partly be due to methodological differences in saliva sampling. In this study we wanted to see to what extent these variations are due to repetition and prestimulation. 9-yr-old schoolchildren (n: 41, 23 girls and 18 boys), who had never had saliva samples taken before, participated in the present study. The repetition tests were carried out between 9 and 11 a.m. on three subsequent days. The effect of prestimulation was tested 4 days later with the same subjects. The buffer capacity of the samples was determined electrometrically immediately after sampling using the commercial Dentobuff-test. The intraindividual analysis revealed a significant increase in flow rate but not in buffer capacity along with repetition of the sampling. The analysis further showed that the buffer capacity of children accustomed to saliva sampling increased significantly through prestimulation of 1 min. The use of prestimulation resulted in substantial changes also in the frequency distribution of the buffer capacity values. Our results emphasize the importance of proper sampling conditions in saliva collection when screening children for high caries risk.

Buffers

Treatment of reciprocal clicking of the temporomandibular joint with a repositioning appliance and occlusal adjustment--results after four and six years.

A follow-up study was performed on 12 subjects with unilateral and five subjects with bilateral reciprocal clicking and symptoms of craniomandibular disorders. After treatment for, on average, three months with a repositioning appliance, followed by occlusal adjustment, patients were examined every six months initially and, after the first two years, once a year. During the follow-up period, occlusal stability was maintained by readjustment and use of full occlusal splints at night, when needed. After four years there was a 57% success rate with regard to treated joints. After six years, the success rate had decreased to 41%. However, even the eight unsuccessful cases, with persistent clicking and adjusted occlusion, managed well with regard to other clinical findings and subjective symptoms. It is concluded that repositioning splint therapy followed by occlusal adjustment seems appropriate for elimination of subjective symptoms and clinical signs even though repositioning may subsequently fail. Results seem to remain relatively unchanged for several years but some decline in the success rate may occur with time.

Adolescent

Effect of elimination of occlusal interferences on signs and symptoms of craniomandibular disorder in young adults.

Sixty-two dental students judged not to be in need of treatment for craniomandibular disorder (CMD) were randomly divided into two groups, one receiving occlusal adjustment and the other mock adjustment. A double-blind study design was applied. After 2 years of education in dentistry, including courses in stomatognathic physiology, the increase in the subjective symptoms of CMD was significantly greater in the placebo control group than in the treatment group. The difference between the groups in the increase of sites tender to palpation was less clear, showing only a trend. However, the increase was statistically significant within the placebo group but not within the treatment group. Prophylactic occlusal adjustment thus appears to be effective in reducing the occurrence of symptoms of CMD, and possibly also the occurrence of clinical signs.

Adult

Salivary parameters and efficiency of dietary instructions to reduce sugar intake among 7-8-year-old schoolchildren.

The efficiency of dietary instructions was tested in a group of 7-8-year-old schoolchildren. The instructions were given in the presence of the child's mother either verbally (control group n = 14) or both verbally and written (test group n = 12). For this purpose the salivary status of the first grade pupils (n = 79) of a primary school in Turku was screened. Salivary flow, buffer capacity, sucrase activity, lactobacillus, yeast and S. mutans counts were determined. Children whose salivary lactobacillus count was over 10(4) CFU/ml (n = 32) were selected for the present study. The efficiency of the dietary instructions was measured as a reduction of salivary lactobacilli. There were no differences in the number of children with reduced LB counts or in the caries increment of 1 yr between the test and the control groups. However, children who succeeded in reducing the number of salivary lactobacilli (42%) within 4 wk revealed a significantly (P less than 0.05) lower caries increment after 1 yr than the rest of the subjects. We concluded that children whose lactobacillus counts were reduced by the dietary instructions developed significantly less caries than children whose lactobacillus counts remained high after the instruction. No additional effect on lactobacillus counts and caries increment could be demonstrated by supplementing the verbal dietary counseling with written instructions.

Bacteria

Ca2+- and Mg2+-activated ATP hydrolysis in human tooth pulp.

ATP-degrading enzyme activities in pulps of healthy and carious human teeth were quantitatively analyzed using the bioluminescence method. The patterns of both Ca2+- and MG2+-activated ATP hydrolysis resembled each other. Increased enzyme activities were observed during injured states.

Adenosine Triphosphatases