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Y Lange

Publications and source records attributed to Y Lange.

60 records · Page 4Linked to original sources
PubMed1972

Cation transport in dog red cells.

Studies have been made on the cation transport system of the dog red cell, a system of particular interest because it has been shown that there is a marked dependence of cation fluxes on the cell volume. We have found that a 10% decrease in cell volume causes a large increase in 1 hr uptake of (24)Na as well as a considerable inhibition of (42)K uptake. This effect cannot be produced by a difference in medium osmolality but rather requires the cell volume to change. Dog red cell uptake of (24)Na is not inhibited by iodoacetate. Phloretin inhibits (24)Na uptake and lactate production, and virtually abolishes the volume effect on Na uptake. These several observations may be accounted for in terms of a working hypothesis which presupposes a cation carrier complex which pumps K into and Na out of cells of normal volume. When the cells are shrunken the carrier specificity shifts to an external Na-specific mode and there is a large increase in (24)Na uptake, driven by the inwardly directed Na electrochemical potential gradient.

Animals↗
PubMed1970

Cellular inhomogeneity in dog red cells as revealed by sodium flux.

Unidirectional (24)Na fluxes across the dog red blood cell membrane were measured. The kinetics were incompatible with a single time constant but could be accounted for in terms of a two-series compartment cell model, with approximately 1% of cell Na in the outer compartment. Dog red blood cells are known to be inhomogeneous in their Na and K permeabilities. Theoretical analysis showed that such cellular inhomogeneity in the Na permeability coefficient might in principle account for the flux data. In order to evaluate the inhomogeneity effect, a technique based on the differential response of cells suspended in isosmolar high K buffers was devised to measure the variations in Na permeability in the cell population. A variation in the Na permeability coefficient of approximately 30% was found. This inhomogeneity is insufficient to account for the flux data.

Animals↗