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Biomedical subjects

Y Kudoh

Publications and source records attributed to Y Kudoh.

97 records · Page 6Linked to original sources
PubMed1982

Serological comparison of two collections of Vibrio cholerae non O1.

Two large, independently obtained collections of vibrio cholerae non O1, containing 59 and 67 reference strains, respectively, were compared serologically in four laboratories. Twenty strains in each collection were considered identical, and an additional 16 strains in each collection were probably identical. Twenty-eight unique strains were identified, and inconclusive results were obtained with 25 strains. Nine strains were not considered V. cholerae in at least one testing laboratory. Of these, five showed insufficient correspondence to V. cholerae O1 by DNA hybridization to be considered V. cholerae.

Agglutination Tests↗
PubMedDate not supplied

Regional myocardial ischemia: characterization of temporal, transmural and lateral flow interfaces in the porcine heart.

The three-dimensional distribution of coronary flow and tissue adenosine triphosphate was characterized in the anaesthetized open pig chest after 10, 15, 30, 45, 60 and 120 min of coronary artery ligation. Radioactive microspheres were given at the onset (153-gadolinium) and end (113-tin) of ischemia. A simultaneous multiple biopsy device was used to obtain 50 contiguous transmural biopsies from each heart over an area (40 mm X 28 mm) of tissue which incorporated the centre of the ischemic zone, a lateral interface of injury and normally perfused tissue. Frozen biopsies were lyophilized, subfractionated into 2 mm transmural sections, and taken for radioactive counting. There was little or no detectable collateral flow in the ischemic zone. There was a sharp lateral flow interface which fell from 2.2 +/- 0.04 mL/min/g in normally perfused tissue to less than 2% of this value over a transition zone of less than 2 mm width. Myocardial adenosine triphosphate content showed a similar sharp interface and was severely depressed throughout the ischemic zone at all the ischemic times studied. During the 2 h ischemic period there was no increase in flow to any part of the ischemic zone and there was no change in the position, or sharpness, of the lateral interface. The absence of either lateral or transmural borderzones of intermediate injury in the pig makes pharmacological infarct size limitation highly improbable in this species.

Adenosine Triphosphate↗
PubMedDate not supplied

Inability of metoprolol to achieve a sustained limitation of infarct size 24 h after coronary artery embolization in the closed chest dog.

Studies were undertaken to ascertain whether metoprolol, a beta 1-selective adrenergic blocking agent, could offer a limitation of myocardial injury throughout a 24-h period of coronary embolization in the dog. Regional myocardial ischaemia was induced through the use of a bead embolization technique which did not require thoracotomy. In order to delineate the zone at risk of infarction (hypoperfused area), radioactive microspheres (141Ce) were administered intraventricularly immediately after embolization. In the drug-treated group (n = 8) metoprolol administration was initiated by an intravenous bolus injection (0.3 mg X kg-1). This was followed by a continuous infusion (0.003 mg X kg-1 X min-1) during the 24-h experimental period. In the control group (n = 8) saline was administered throughout the 24-h period. Electrocardiographic activity was monitored throughout the experiment and this confirmed the negative chronotropic and antiarrhythmic properties of metoprolol. After 24 h, the hearts were excised and transverse myocardial sections (3 mm) prepared. Areas of necrosis were visualized by tetrazolium staining and risk zones were defined by microsphere autoradiography. In the control and metoprolol-treated groups, 73.3 +/- 7.7% and 68.2 +/- 6.1% of the risk zone became necrotic, respectively. There was no significant difference between these groups.

Animals↗