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Biomedical subjects

Y Kondo

Publications and source records attributed to Y Kondo.

At least 919 records · Page 51Linked to original sources
PubMed1981

Neurophysin biosynthesis in vitro in oat cell carcinoma of the lung with ectopic vasopressin production.

The incorporation of labeled compounds into neurophysins of a transplantable human oat cell carcinoma of the lung with ectopic vasopressin production was studied in vitro. Neurophysins in cell extracts and in incubation media were isolated by immunoprecipitation and analyzed by sodium dodecyl sulfate (SDS)-polyacrylamide gel electrophoresis. When cells were incubated with L-[35S]cysteine for 12 h, SDS-polyacrylamide gel electrophoresis of the immunoprecipitates from cell extract and medium resolved two forms of neurophysins with apparent molecular mass of 10,000 (10K) and 20,000 (20K). Both forms of [35S]-neurophysins were completely displaced from the immunoprecipitates by excess human neurophysin. Incubation of cells with L-[35S]cysteine and D-[3H]-glucosamine hydrochloride revealed that glucosamine was incorporated into the 20K neurophysin region, but not into 10K species. To observe the kinetics of labeling of the two forms of neurophysins, cells were incubated with L[35S]cysteine for varying periods of time. After short labeling periods, most of the radioactivity resided in 20K species, which plateaued after 1 h, whereas 10K neurophysin progressively increased in its height. When cells were chased with unlabeled cysteine after the exposure to a short pulse of labeling, 20K neurophysin peak gradually decreased with an apparent initial half-life of 1 h. In contrast, the label in 10K neurophysin steadily increased, which exceeded the former by 3 h of chase. Analysis of 20K neurophysin in cell extract by isoelectric focusing on polyacrylamide gel demonstrated that it was principally composed of a protein with an apparent isoelectric point (pI) of 5.7. These results suggest that neurophysin is synthesized in ectopic vasopressin-producing tumors by post-translational processing from a glycosylated proneurophysin with an apparent molecular mass of 20,000 daltons and a pI of 5.7.

Animals↗
PubMed1981

Immunohistochemical localization of dipeptidyl aminopeptidase IV in rat kidney, liver, and salivary glands.

Specific antibodies directed against dipeptidyl aminopeptidase (DAP) IV prepared from rat and pig kidneys were produced in rabbits. Using a peroxidase-labeled or fluorescein isothiocyanate-labeled antibody method, localization of DAP IV was investigated in various tissues of the rat (kidney, liver and salivary glands). DAP IV was mainly localized in the brush border of the proximal tubules (kidney), in the cell membrane around bile canaliculi of hepatic cells (liver), and in the cell membrane of serous acinar cells (parotid and submaxillary glands). These results substantiate that DAP IV is a membrane-bound peptidase.

Animals↗
PubMed1981

Aortic insufficiency caused by Takayasu's arteritis without usual clinical features.

A 30-year-old woman with rapidly progressive, fatal aortic insufficiency was studied. Pathologic studies revealed that the aortic insufficiency was caused by dilation of the aortic root due to Takayasu's arteritis that, unusually, was confined to the ascending aorta and aortic arch. When other diseases that bring about aortic insufficiency can be excluded, Takayasu's arteritis should be considered, even if most of its manifestations are absent.

Adult↗
PubMed1981

Inappropriate secretion of antidiuretic hormone in nude mice bearing a human bronchogenic oat cell carcinoma.

A 58-year-old man with bronchogenic oat cell carcinoma developed a typical syndrome of inappropriate secretion of antidiuretic hormone. The tumor tissue obtained at autopsy had been serially transplanted in nude mice for more than four years with 20 passages. The levels of vasopressin were remarkably increased in the plasma of nude mice bearing this tumor [24.4 +/- 18.3 (S.D.) pg/ml, n = 3] as well as in the tumor tissues ]134.3 +/- 72.2 ng/g, n = 3]. Furthermore, human nicotine-stimulated neurophysin was detected in both plasma and tumor tissues (7.4 +/- 3.7 ng/ml, n = 3, and 2.28 +/- 0.90 micrograms/g, n = 3, respectively). On ad libitum intake of water, nude mice bearing this tumor excreted significantly less urine with higher sodium concentration than did controls, but serum sodium concentrations did not differ from those of controls. When tumor-bearing mice were hydrated with 2 ml of water twice a day i.p., their diuretic response was found to be suppressed in parallel with the tumor size. However, these mice did not become hyponatremic because they drank less water. When a larger amount of water was loaded which could not be compensated by restriction of water drinking, serum sodium concentrations were markedly decreased. On the basis of these results, the lung cancer, when transplanted into nude mice, produced and secreted its own antidiuretic hormone, which induced inappropriate secretion of antidiuretic hormone in the mice. These mice may provide a useful experimental model for the study of excessive secretion of antidiuretic hormone and associated pathophysiological disorders.

Animals↗
PubMed1981

Serum sialyltransferase and liver catalase activity in cachectic nude mice bearing a human malignant melanoma.

Cachexia is rare in nude mice bearing human malignant tumors even when the transplanted tumors become as large as the body size of the host. In our series on heterotransplantation of a variety of human malignant tumors into nude mice, a malignant melanoma (SEKI) was found to induce severe body weight loss in the host at the early stage of transplantation. There was no electrolyte disturbance, hyper- or hypoadrenocorticism, hyperthyroidism, or destruction of cells of vital organs to account for the weight loss. Moreover, no evidence was obtained for concomitant infection with bacteria, Mycoplasma or fungi. These cachectic mice revealed remarkably increased levels of serum sialyltransferase and decreased liver catalase activity. The removal of tumor tissues from these mice resulted in prompt recovery of body weight, serum sialyltransferase, and liver catalase activity within 1 to 2 weeks. On the basis of the results obtained, the SEKI melanoma was thought to have produced a pathophysiological state in host nude mice which was very similar to that of cachexia in cancer patients. Nude mice bearing transplants of SEKI melanoma may provide a useful system for the study of cancer cachexia in humans.

Animals↗
PubMed1981

[Study on radiosterilization of crude drug pill involving bezoar bovis--radiolysis of bilirubin and cholic acids in hydrous starch pellet (author's transl)].

Radiolysis of bilirubin and cholic acids (cholic acid, desoxycholic acid and lithocholic acid) in hydrous pellet have been investigated with following parameters, which were hydrous content, radiation dose and the dose rate, to discuss the application of gamma-irradiation for sterilization of crude drug pill involving bezoar bovis. At 774 C/kg (3.0 MR) irradiation for 5% and 10% hydrous contents pellets, the radiolysis percent of those components were less than 5%. However, the higher hydrous pellet, the radiolysis percents of those are more increase. At the same irradiated condition for 20% hydrous contents pellets, the radiolysis percents of those were 15-22%. The hydrous percent of commercial crude drug pill involving bezoar bovis are about 9%, so that the radiolysis of those components will be less than 5% on the sterilization. The radiolysis percent of bilirubin are constant to variation of radiation dose rate between 51.6-722.5 C/kg.hr (0.2-2.8 MR/hr). But, the values of cholic acids don't definite such as that of bilirubin, because of larger analytical error.

Animals↗
PubMed1980

Effect of thyrotropin on cell orientation and follicle reconstruction in rotated suspension culture of hog thyroid cells.

Isolated porcine thyroid cells were cultured in suspension by rotation on a gyratory shaker. In the absence of TSH, the cells associated into small, globular, and hollow aggregates or pseudofollicles and had microvilli only at the outer surface of the pseudofollicles which faced the culture medium. These pseudofollicles were inactive in terms of thyroglobulin synthesis and iodination. In the presence of TSH, cell aggregates were enlarged to 1 mm in diameter. Inside the aggregates. Inside the aggregates, the cells were reorganized into numerous functional follicles in which microvilli were oriented towards the follicular lumen. In both the presence and absence of TSH, Golgi apparatuses were found in the cytoplasm near the cell periphery bearing microvilli. These findings indicate that the TSH effects on thyroid cell morphology are to arrange the polarity of follicular cells in a proper direction and to enlarge the aggregates they form.

Animals↗
PubMed1980

Structure-activity relationships for the inhibition of formation of acid-fastness im mycobacterial organisms by coumarins and cinnamates.

The relationships between the structure and inhibitory activity against the formation of acid-fastness in mycobacterial organisms were investigated in naturally occurring or synthetic coumarins and cinnamates. The presence of an OH-group at C7 in the coumarin nucleus proved to be essential for the inhibitory activity. Likewise, the presence of a para-OH-group in cinnamates is also essential. Reduction of a conjugated alpha, beta-double bond in both series resulted in a decrease of the ininhibitory activity. Shortening of the side chain of cinnamates resulted in total loss of the activity. The grouping -C-C-CO2R appeared to be necessary for retaining the inhibitory activity.

Acids↗
PubMed1980

[Effect of transection on gastrointestinal electric activities (author's transl)].

Alterations in the pattern of basic electric rhythm of the stomach and jejunum were studied in a total of 12 dogs subjected to following procedures; (a) transection and close contact repair of the entire seromuscular layer in the upper jejunum; (b) transection and close contact repair of the seromuscular layer in the stomach; (c) transection and interposition repair with alpha cyano acrylate in the stomach. Jejunal transection has resulted in permanent interruption in coupling of the basic electrical rhythm irrespective of repair procedures of the muscle layers. Gastric transection has revealed interruption in coupling of the basic electrical rhythm temporarily, but coupling phenomenon was observed in about 14 days. Gastrin transection with alpha cyano acrylate interposition has resulted in interruption in coupling of the basic electrical rhythm when the animal is maintained in fasting state. However, coupling has occurred immediately after the feeding or intragastric balloon distension. These results indicate that there exists some difference between the stomach and the jejunum in the effects of transection on electrical activities. Coupling phenomenon of the basic electrical rhythm observed in the transected stomach is probably due to an interaction of nervous system or a mechanical stimulus acting across the transection line.

Animals↗