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Biomedical subjects

Y Kondo

Publications and source records attributed to Y Kondo.

At least 865 records · Page 48Linked to original sources
PubMed1984

Studies on a new nucleoside antibiotic, dapiramicin. II. Isolation, physico-chemical and biological characterization.

New nucleoside antibiotics, dapiramicins A and B produced by a Micromonospora sp. SF-1917, have been isolated by column chromatography on Diaion HP-20 and silica gel. Physico-chemical properties suggested that they are disaccharide nucleosides. Dapiramicin A underwent epimerization, under acidic condition, into epidapiramicin A. Although dapiramicin A generally exhibits no in vitro activity, it is very effective against the sheath blight of rice plants caused by Rhizoctonia solani in a green house test.

Aminoglycosides↗
PubMed1984

[Mixed mesodermal tumor of the ovary].

A 68-year-old woman with a malignant mixed mesodermal tumor of the ovary is reported. Her clinical course worsened rapidly and she died 3 months after admission. At autopsy, the pelvic cavity was seen to be filled with a huge tumor mass. Histologically, the tumor contained malignant epithelial and mesenchymal elements. The epithelial component was an adenosquamous carcinoma. The stromal element was comprised mainly of anaplastic, undifferentiated spindle cells. A variety of malignant mesenchymal elements were also present. They resembled rhabdomyosarcoma, liposarcoma, chondrosarcoma, fibrosarcoma, and myxosarcoma . A review of the Japanese literature concerning this tumor is also presented.

Aged↗
PubMed1984

Acute antihypertensive effect of nifedipine by sublingual route in cases with clinically severe systolic hypertension. A study up to 4 h after administration.

In a multi-center study, nifedipine (Bay a 1040, Adalat) (10 mg capsule) was administered, in liquid form and via sublingual route, to 22 cases who were diagnosed to have clinically severe systolic hypertension, and the depressor effect of the treatment was studied over a period of 4 h. In patients of "emergent" admission (cerebral hemorrhage n = 8, cerebral thrombosis n = 4, subarachnoid hemorrhage n = 1, renal failure n = 6, essential hypertension n = 3), 3 bouts of blood pressure measurement at intervals of 10-15 min during the control period were carried out. In case of systolic blood pressure higher than 200 mmHg, at least one time, nifedipine at a dose of 10 mg was sublingually administered. Thereafter, blood pressure and pulse rate were recorded at intervals of 15 min up to the end of the first one hour, then at intervals of 30 min up to the end of 4 h. Results were as follows. In terms of the average values for all cases, blood pressure fell to near lowest levels by the end of about 30 min after the administration and it stayed at near lowest levels up to the end of 120-240 min. During this period of time, pulse rate remained substantially unchanged. In terms of the pattern of the blood pressure fall, all cases could be classified generally into two types, namely (a) the "dip" group (7 cases) in which blood pressure fell to the lowest level to form a "dip" and remained below the control level, although it showed a trend to return to control level for 4 h and (b) the "flat" group (15 cases) in which blood pressure declined gradually for about 1 h and, then, remained low or below the control level, although it showed a trend to return to control level throughout the rest of the observation period of 4 h.

Administration, Oral↗
PubMed1984

Multiplication of Brucella canis in male reproductive organs and detection of autoantibody to spermatozoa in canine brucellosis.

Orchitis, epididymitis and prostatitis have been reported in male dogs infected with Brucella canis (B. canis), but the pathogenesis of infertility in male dogs has not been clarified yet. We examined localization of B. canis in the tissue of infected male reproductive organs and production of autoantibody to spermatozoa in male dogs by immunofluorescence and unlabeled antibody peroxidase-antiperoxidase (PAP) methods and electron microscopy. B. canis were found in the cytoplasm of macrophages and epithelial cells in testis, epididymis and prostate. Particularly in the prostate, B. canis multiplied in the cytoplasm of epithelial cells and emerged in the glandular lumen with destroyed epithelial cells. Head-to-head agglutination of spermatozoa was found in the semen, urine and epididymal duct with varying degrees of intensity among the infected dogs. Appearance of the spermagglutination began following the detection of B. canis in urine and semen, suggesting invasion of the organisms in male reproductive organs. In the sera from the dogs orally inoculated with B. canis, (Ig M), Ig G and Ig A anti-spermantibodies were detected in parallel with the appearance of the serum spermagglutinating activity. The heads of agglutinated spermatozoa in the epididymal duct and semen were coated with Ig A antibody, which is considered to be anti-spermautoantibody locally produced. The target of these circulating and local antibodies was acrosome of the dog spermatozoa and spermatids. It seems probable that multiplication of B. canis in epithelial cells is the direct cause of damage to the infected cells, and the damage acts as a trigger of the production of autoantibody to spermatozoa.

Animals↗
PubMed1984

[Clinical evaluation of tissue polypeptide antigen in patients with esophageal, stomach and colon cancer].

Serum tissue polypeptide antigen (TPA) levels were measured in 33 patients with esophageal cancer, 39 with stomach cancer and 50 with colon cancer. At the same time five glycoproteins, namely immunosuppressive acidic glycoprotein (IAP), alpha 1-antichymotripsin (alpha 1-ACT), acid soluble glycoproteins (ASP), sialic acid and carcinoembryonic antigen (CEA), were measured for comparison. The mean TPA values were 59.0 +/- 15.4 U/l in 61 normal subjects, 103.6 +/- 104.2 U/l (positive rate, 24.2%) in esophageal cancer patients, 111.9 +/- 49.8 U/l (71.8%) in stomach cancer patients and 124.8 +/- 195.5 U/l (40%) in colon cancer patients. The serum TPA levels in patients with stomach cancer rose with an increased number of involved lymph nodes and with a higher degree of infiltrative growth and increased with the advancement of tumor growth postoperatively. Serum TPA levels correlated well with those of alpha 1-ACT, IAP and ASP in stomach cancer patients and with those of CEA, ASP and sialic acid in colon cancer, but not in esophageal cancer patients. It is suggested that the serum TPA might represent one of the reactant proteins and/or tumor-associated antigens that appear to be dependent upon the cancer status.

Adult↗
PubMed1984

[Efficiency of tritium enrichment by electrolytic cell with multi-nickel-plates electrode and its application to the determination of tritium in environmental water].

For the purpose of speeding up the tritium enrichment by electrolysis, we have produced an electrolytic cell with the multi-plate-electrode system instead of the commonly used single-plate-electrode, and examined the efficiencies for the tritium enrichment under the conditions of different current densities and electrode gaps. From the results, the tritium recovery and the separation factor beta were found to be maximized under the condition of 70 mA/cm2 of current density and 1.6 mm of electrode gap, and they were 90 percent and 23, respectively. Using this cell, it took 28 hours to reduce 100 ml of a sample water to 10 ml, and took 2 days, including the time required for other operations, to determine the tritium concentration of 1.85 Bq/l (50 pCi/l) with the counting error of within +/- 10 percent. This method has been applied to determining the tritium concentrations of environmental samples from Yamato River region during July 1981-February 1983. They were in the range of 1.11-9.48 Bq/l (30-256 pCi/l).

Electrodes↗
PubMed1984

[Nutritional support in surgery: with special reference to fat emulsion and carnitine].

Energy metabolism of fat emulsion has been studied in rats with various conditions including postoperative catabolism, chronic and acute hepatic dysfunction, diabetes and sepsis. The results indicate that fat emulsion is rapidly eliminated from the blood stream and metabolized readily even in such conditions. Based on these findings, we have actively employed fat emulsion clinically as energy source. In septic rats, it was demonstrated that levels of carnitine decreased and that this decrease was based upon a decrease of synthesis. When carnitine was administrated together with fat emulsion, the energy metabolism returned to approximately normal level. This report also describes the tissue concentrations of carnitine in diabetic rats and the blood and urine concentrations of carnitine in postoperative patients.

Animals↗
PubMed1983

[Evaluation on the interaction between thyroxine binding globulin (TBG) and thyroxine (T4) in healthy subjects and pregnant women].

A very simple method for the evaluation of the interaction between thyroxine binding globulin (TBG) and thyroxine (T4) has been developed and evaluated in 6 healthy subjects and 5 pregnant women. Rabbit anti-human TBG antiserum was precipitated with 50% ammonium sulfate followed by passing a DEAE-cellulose column. Immunoglobulin G (IgG) fraction thus prepared was covalently attached on a Sepharose CL-4B and used as an immunoadsorbent of serum TBG. After treatment with charcoal to remove endogeneous thyroid hormones, 10 microliter of sera from 6 healthy subjects and 5 pregnant women were incubated with 125I-T4 and various concentrations of cold T4 followed by immunoadsorption with anti-TBG-Sepharose, and association constants (Ka) between TBG and T4 were calculated in each subject using Scatchard's plot. The Ka value thus obtained in 6 healthy subjects was 1.47 X 10(8) M-1 (SD = 0.24), and in 5 pregnant women it was 0.82 X 10(8) M-1 (SD = 0.21) which was significantly lower than that of healthy subjects (P less than 0.001). Our present method does not need purification of TBG from serum to investigate its functions, especially in relation to the binding with thyroid hormones. Also, only a very small amount (100 approximately 150 microliter) of serum is enough for the calculation of Ka between T4 and TBG. The whole procedure is very easy to perform and can be done in a relatively short time, and therefore we consider this method clinically relevant and useful.

Adult↗