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Y Konaka

Publications and source records attributed to Y Konaka.

32 records · Page 2Linked to original sources
PubMed1983

B-cell malignancy and monoclonal gammopathy, and idiotype of cell surface and serum immunoglobulin.

We studied cell surface membrane immunoglobulins (SmIg), intracytoplasmic Ig (CIg) and monoclonal Ig (M protein) in patients with the B-cell series malignancies; malignant lymphoma, chronic lymphocytic leukemia (CLL), Waldenström's macroglobulinemia (WM), multiple myeloma (MM), and monoclonal gammopathy (MG) associated with Sjögren's syndrome (SS). The patients were examined for idyotype (Id) determinants on the cell surface and in the cytoplasm of malignant cells as well as serum M proteins. Twenty-two patients had malignant lymphoma with monoclonal SmIg. The malignant cells of 11 patients contained CIg; four of these 11 patients had a small amount of serum M protein. SmIg of the IgM class was most frequent (12/22) and the lambda type light chain was predominant (kappa/lambda ratio = 4/8). By means of rabbit anti-Id antisera prepared against M proteins, Id determinants were demonstrated on the cell surface and in the cytoplasm of malignant cells as well as in the serum M proteins, indicating that the malignant cells proliferated monoclonally producing monoclonal IgM on the cell surface and in the cytoplasm and secreting it to the serum. Six CLL patients were included in our series; one manifested monoclonal IgM in the serum. The Id determinants of M protein was detected on the cell surface and in the cytoplasm of pathologic cells in the peripheral blood. Eight of ten WM patients were examined for SmIg; all had monoclonal SmIg on the surface of cells from peripheral blood or bone marrow. Id determinants were detected on the cell surface and in the cytoplasm of malignant cells in four WM patients. There were 42 patients with MM; 10 of them were examined for SmIg and three of these had monoclonal SmIg on plasma cells derived from the bone marrow. By immunofluorescent and mixed rosette methods, Id-bearing B (0-20%) and T cells (0-2%) were identified in the peripheral blood of MM patients who had no plasma cells in the peripheral blood. Our findings suggest that a monoclonal change may occur at the B-cell level and that Id-bearing T cells may exist as regulatory T cells. Ten SS patients had M proteins. Non-IgM class M proteins (2 IgG and 4 IgA) were more frequent than the IgM class (3 IgM). Four M proteins with rheumatoid factor (RF) activity were found among these 10 M proteins.(ABSTRACT TRUNCATED AT 400 WORDS)

Adult↗
PubMed1981

Anti-Thy-1-mediated T cell activation. Role of soluble factors and expression of interleukin 2 receptors on T cells.

Rabbit anti-Thy-1 antibody bound to T cells induces the appearance of T cell growth factor (interleukin 2, IL2) receptors and the production of IL2. IL2 receptors induction by this means occurs within a short time span, and independent of any demonstrable accessory cell. A source of exogenous or endogenous IL2 is apparently the only requirement for proliferation after these receptors are expressed. IL2 production, on the other hand, is accessory cell-dependent, and this help is mediated via Fc receptors on the accessory cell. IL2 production in this model is demonstrable at 4-6 h, or earlier, and is proliferation-independent. X-irradiation actually augments apparent IL2 production. The data are consistent with the hypothesis that a differentiated subset of T cells in any mixed population is responsible for IL2 production.

Animals↗
PubMed1980

Mitogen-initiated synthesis and secretion of T cell growth factor(s) by a T-lymphoma cell line.

Of 12 T-lymphoma cell lines investigated, one line, EL-4 azgr (Thy-1+, Lyt-1+, Lyt-2-3-,Ia-, T-200+, sIg-, and FcR-) and, to a lesser extent, the parental cell line, EL-4, produced T cell growth factor(s) (TCGF) when stimulated by the T-cell mitogen concanavalin A (Con A). Induced production of TCGF-E was detected by 6 h and maximal at 18-24 h. Purified TCGF-E from this source had an approximately 30,000 mol wt and the biological activity of TCGF produced by whole spleen cells, including: augmentation of T cell-mitogen responses, cytotoxic T lymphocyte (CTL) proliferation support dependence, augmented generation of CTL, lack of strain specificity, and failure to stimulate resting T cells. TCGF-E is neither synthesized or secreted by this lymphoma cell line unless stimulated by Con A. X-irradiation up to 7,000 rad failed to inhibit synthesis and secretion. These observations have a practical application in providing a relatively homogeneous clonal cell product for T cell culture support and for structural and functional studies of the TCGF molecule(s). They suggest also a model for examining mechanisms of triggering production and secretion of a regulatory molecule that controls T cell functions.

Animals↗
PubMed1978

Leukemic lymphosarcoma (LLS) with monoclonal IgM: idiotypic specificity on the cell surface and in the cytoplasm of lymphosarcoma cells.

A case of leukemic lymphosarcoma with a small amount of monoclonal IgM in the serum is described. Immunologic investigations showed that about 10% of the abnormal cells formed EAC rosettes, that most of these cells had IgMlambda on the cell surface, and that 40% of them contained IgMlambda in the cytoplasm. Monoclonal IgM was detected in the 19S fraction of the serum despite the lack of monoclonal change as determined by serum electrophoresis or immunoelectrophoresis. The common idiotypic specificity was found in the monoclonal serum IgM, surface Ig, and cytoplasmic Ig of lymphosarcoma cells using antiidiotypic antiserum prepared against purified serum IgM. After chemotherapy, cells with idiotypic determinants disappeared and the serum IgM changed from a monoclonal to a polyclonal distribution.

Adult↗