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Biomedical subjects

Y Komine

Publications and source records attributed to Y Komine.

At least 55 records · Page 3Linked to original sources
PubMed1989

Retention behavior of cardiac steroids using cyclodextrin in the mobile phase in high-performance liquid chromatography.

The retention behaviour of twenty cardiac steroids and four fluorescent derivatives was examined by the addition of cyclodextrin to the mobile phase in reversed-phase high-performance liquid chromatography. The addition of a suitable cyclodextrin improved the separation of isomeric cardiac steroids. The steroid A/B ring junction is the most important factor in the choice of the optimum cyclodextrin to be added; the C/D ring junction is less important. The hydroxyl group at the 3- or 12-position of the steroid enhanced the changes in retention times of these compounds. The effect of an unsaturated lactone ring at the 17 beta-position on the retention in the presence of cyclodextrin was also determined with cardenolide (five-membered ring) and bufadienolide (six-membered ring) but little difference was observed. Isomeric cardiac steroids, whose separation has not been done by the conventional method, were clearly separated by this method. The fluorescence intensity of 3-(1-anthroyl)-cardiac steroid was enhanced by the addition of cyclodextrin to the mobile phase.

Animals↗
PubMed1989

Monoclonal antibody EMR1a/212D recognizing site of deposition of extracellular lipid in atherosclerosis: purification and characterization of the antigen.

The monoclonal antibody EMR1a/212D, which recognizes the extracellular matrix in which lipids are deposited in atherosclerotic lesions, was developed previously. The macromolecule containing the epitope recognized by this antibody was purified from serum of WHHL rabbits, antigenic material similar to that found in serum and atherosclerotic plaques. The antigenic material was not associated with lipoproteins in serum. The antigenic material was purified in a single band by SDS-PAGE followed by DEAE-Sepharose CL-6B column chromatography (Pharmacia, Uppsala, Sweden), EMR1a/212D coupled immunoaffinity column chromatography, and HPLC equipped with a TSK gel G3000SWXL (Toso, Japan) column. The purified antigenic material was a glycoprotein of molecular weight 66 kd. It was remarkably high in glutamic acid and aspartic acid but low in arginine and lysine. It had an isoelectric point of pH from 5.4 to 5.9. It contained 22.2 mg of sugar per 100 mg protein, and sialic acid at least expressed the activity of the epitope because the antigenic activity was decreased by neuraminidase treatment.

Amino Acids↗
PubMed1988

A nicked group II intron and trans-splicing in liverwort, Marchantia polymorpha, chloroplasts.

The chloroplast gene rps12 for ribosomal protein S12 in a liverwort, Marchantia polymorpha, is split into three exons by two introns, one of which (intron 1) is discontinuous. Exon 1 of rps12 for the N-terminal portion of the S12 protein is far from exons 2 and 3 for the C-terminal portion on the opposite DNA strand. S1-nuclease protection analysis and Northern hybridization with RNA isolated from the liverwort chloroplasts showed that: (i) the exons 1 and 2-3 of the rps12 gene with the neighboring genes were transcribed separately, (ii) the trans-splicing of intron 1 occurred after the processing of two primary transcripts to two pre-mRNAs, and (iii) there was no particular order for the splicing of intron 1 (trans) and intron 2 (cis) in the rps12 gene. We propose a bimolecular interaction model for trans-splicing by assuming that intermolecular base pairings between two pre-mRNAs result in the formation of the structure typical of group II introns except for disruption in the loop III region. This structure could be constructed in intron 1 of tobacco rps12 gene.

Base Sequence↗
PubMed1988

Single photon emission computed tomography (SPECT) in a patient with Wilson's disease.

Single photon emission computed tomography (SPECT) with 133Xe inhalation was studied in a patient with Wilson's disease, who had the low-density lesions in the bilateral ganglia and a small calcified lesion in the left basal ganglia on computed tomography (CT). SPECT showed the regional cerebral blood flow (rCBF) of the basal ganglia area. The values of the rCBF in these areas are 77 to 91 ml/100g/min in the right, and 56 to 77 ml/100g/min in the left. Further study is needed to prove the pathological role of rCBF for the basal ganglia lesion in the patient with Wilson's disease.

Adolescent↗
PubMed1987

The primary structure of the myosin head.

The sequence of the NH2-terminal 808 amino acid residues of chicken pectoralis muscle myosin head was determined. Three characteristic 20-, 23-, and 50-kDa fragments were isolated from a digest of myosin subfragment 1 (S1) by gel filtration on a Sephadex G-100 column in the presence of 5 M guanidine hydrochloride, followed by anion-exchange chromatography on a QAE-Sephadex A-50 column in the presence of 8 M urea. The fragments were sequenced completely by conventional methods. Peptides overlapping the 23- and 50-kDa fragments and also overlapping the 50- and 20-kDa fragments were obtained by cleaving S1 with cyanogen bromide. Comparison of the 23-kDa and 50-kDa sequences with that of the overlapping peptide indicated that no additional amino acid exists between the 23- and 50-kDa fragments and that 5 amino acids exist between the 50- and 20-kDa fragments of S1. Methylated amino acid residues were found at four positions: epsilon-N-monomethyllysine at position 35, epsilon-N-trimethyllysine residues at 130 and 550, and 3-N-methylhistidine at 754.

Amino Acid Sequence↗
PubMed1986

Respiratory myoclonus. Report of a case with electromyographic study.

A case of respiratory myoclonus in a 45 year-old male was studied by electromyography. He complained of anorexia, involuntary swinging movement of the thorax and trunk, and epigastric pulsation. Electromyographic studies showed synchronous rhythmic grouped discharges at the of 2 to 3 Hz from the bilateral 8th to 10th intercostal muscles, the right iliocostalis lumborum muscle, and the left hemidiaphragm only during the expiratory phase. They disappeared on inspiration and forced expiration. Anesthetic block of the left phrenic nerve abolished both the voluntary and myoclonic movements of the left hemidiaphragm. Abnormal excitation of the central nervous system and possible irritation of the afferent pathways were postulated.

Afferent Pathways↗
PubMed1983

Effect of hyperthermic vesical irrigation with bleomycin on the ultrastructure of the well-differentiated tumour and non-tumorous mucosa of the human urinary bladder.

Bladder tumours and non-tumorous bladder mucosa were studied by scanning and transmission electron microscopy in seven patients who had undergone hyperthermic vesical irrigation with bleomycin. The treatment induced sloughing of the outermost tumour cells, an increase of blebs and a decrease of cytoplasmic processes of the deeply located tumour cells as well as cellular degeneration. Although less severe, non-tumorous mucosa showed similar changes. Microvilli also appeared on the superficial cells of non-tumorous mucosa after the treatment. This treatment is effective by inducing cell degeneration and desquamation but not selective to the bladder tumour.

Adult↗
PubMed1981

Transurethral ultrasonotomography of the prostate.

Newly developed transurethral ultrasonic scanners were used to study 20 patients with histologically proven prostatic cancer. Fifty-two patients with or without other prostatic disease were also evaluated. The characteristic findings of prostatic cancer were asymmetry of the shape (85%), ill-defined contour (75%), protruded areas from the boundary (70%), elongation of anteroposterior diameter (65%), and enlargement of the prostate (65%). In addition, transurethral ultrasonography delineated a clearer contour of the prostate compared with the transrectal procedure.

Humans↗