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Biomedical subjects

Y Kito

Publications and source records attributed to Y Kito.

At least 145 records · Page 8Linked to original sources
PubMed1982

[Evaluation of left ventricular morphology and function by cardiac computed tomographic examination].

Usefulness of cardiac computed tomography (CT) in the evaluation of left ventricular (LV) morphology and function was studied in clinical practice. One hundred and forty-nine adult cases of various heart diseases were examined by GE scanner with ungated scans covering whole LV and ECG-gated scans. In gating examination, "long-axial" and "short-axial" slices were scanned and end-diastolic area (EDA), end-systolic area (ESA) and "mean" area (MA) were obtained in each slices. 1) With regard to LV morphology, wall thickening in HCM and wall thinning and mural thrombus in myocardial infarction were easily visualized. 2) LV "mean" volume (LVMV) was easily calculated from the sum of sliced LV volume of ungated scans. The LVMV was well correlated with end-diastolic volume (EDV) obtained by LV angiocardiography (LVG) (r = 0.91), though the former was somewhat smaller than the latter. EDV by CT obtained after the correction with MA/EDA from gating examination showed a better correlation (r = 0.95) and both EDV showed almost the same value. 3) LV ejection fraction (EF) was calculated by exchanging ESA/EDA to ESV/EDV in "long-axial" and "short-axial" gated slices, and "long-axial" EF, "short-axial" EF and mean of both were well correlated with LVEF of LVG (r = 0.73-0.79). 4) LVEF calculated from "long-axial" EDA and ESA by application of "area-length" method was also correlated with EF of LVG (r = 0.68), but these EDV and ESV were smaller than those of LVG due to shorter long-diameter of LV in CT. 5) LV muscle volume was calculated from sum of sliced muscle volumes of ungated scans covering LV, and LV mass was easily evaluated from LV muscle volume and specific gravity of LV muscle. This LV mass was well correlated with that of LVG (r = 0.90) and the absolute values were almost the same.

Adult↗
PubMed1980

Effect of phospholipid and detergent on the Schiff base of cephalopod rhodopsin and metarhodopsin.

In the visual pigment, the chromophore retinal is bound through a Schiff base linkage with the protein moiety, opsin. The secondary interaction of retinal with opsin was studied in cephalopod rhodopsin and its photoproduct, metarhodopsin. The pK of the protonation of the Schiff base in metarhodopsin was affected by the phospholipids and detergents surrounding the protein, and varied between 9.2 and 6.9. Among nonionic detergents, the fatty acid ester of sucrose behaved like phospholipids but other detergents changed the protein conformation so that the pK of the Schiff base in metarhodopsin became nearly equal to the pK of N-retinylidene butylamine. This tendency was manifested in rhodopsin as the formation of a 380 nm pigment.

Animals↗
PubMed1979

Molecular weight and structural studies on cephalopod rhodopsin.

The protein moiety of squid (Watasenia scintillans) rhodopsin has been shown to have a molecular weight of 46 800 by means of amino acid analysis. This value was comparable to the value (51 000) obtained from SDS-polyacrylamide gel electrophoresis. After the squid eyes were incubated at 10 degrees C for 8 days, the rhodopsin showed a molecular weight of 39 000 on electrophoresis. The smaller molecular weight was ascertained by amino acid analysis of the rhodopsin; and may result from autolysis by the lysosomal enzyme. The rhodopsin in rhabdomeric membranes and in detergent solution was treated with chymotrypsin, papain or subtilisin. These enzymes first produced the 39 000 dalton rhodopsin and then cleaved this into the 25 000 and 14 000 dalton peptides without bleaching. The rhodopsin was attacked by proteases and readily lost an approx. 12 000 dalton peptide portion. This portion included the COOH-terminal and was rich in glutamic acid, proline, glycine, alanine and tyrosine residues.

Amino Acids↗