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Biomedical subjects

Y Kitazawa

Publications and source records attributed to Y Kitazawa.

At least 271 records · Page 15Linked to original sources
PubMed1966

Use of water-drinking tonography in mass screening for glaucoma.

The results of mass screening for glaucoma of workers in a factory in Chiba Prefecture, Japan, are reported. At the first-stage examination, tonometry was performed with standardized Schiøtz tonometers on 1,527 subjects (3,054 eyes) with no ocular symptoms. When the tension was 5.0/5.5 gm or higher, the patient was regarded as a preliminary suspect and a detailed ophthalmologic examination was recommended. The second-stage examination, in the University Clinic included campimetry, vision tests, water-drinking tonography, tonometry with applanation and Schiøtz tonometers, funduscopy and gonioscopy. Of the 239 (381 eyes) preliminary suspects, 79 came to the Clinic for the second-stage examination. Water-drinking tonography, done on 104 eyes with tension of greater than 5.0/5.5 gm, revealed that 48 had a Po/C ratio greater than 100, which strongly suggests the presence of glaucoma. In water-drinking tonography, a Po/C ratio greater than 100 was noted in about 34% of 58 eyes with a tension of 4.25/5.5-5.0/5.5 gm, suggesting that the index for separating glaucoma suspects from normals could be brought down to the level of 5.0/5.5 gm. The over-all percentage of glaucoma cases found was 1.3%. The efficacy of water-drinking tonography in mass screening for glaucoma has been discussed.

Adolescent↗
PubMedDate not supplied

Flow cytometric analysis of the direct toxic effects of paraquat on cultured MDCK cells.

We used flow cytometry to assess the cell cycle kinetics of cultured Maden Darby canine kidney cells after exposure to paraquat. Fluorescein diacetate fluorescence was used as a marker of cell viability, while bromodeoxyuridine incorporation was detected with a monoclonal antibody and propidium iodide staining to assess DNA synthesis. Flow cytometry was performed immediately, 48 h and 96 h after exposure to paraquat for 24 h. Lactate dehydrogenase release was also measured to determine the extent of cytolysis. Flow cytometry of paraquat-treated cells showed a marked increase of the S phase population immediately after exposure, at a time when there was no increase of lactate dehydrogenase release. In contrast, the cell cycle profile returned towards normal at 48 and 96 h after paraquat exposure, but lactate dehydrogenase release increased. These findings indicate that paraquat arrested cells in the S phase and that inhibition of DNA synthesis by this agent appeared to influence cell viability because S phase block occurred before cytolysis. In addition, this method proved useful for assessing the effects of paraquat on DNA synthesis by cultured cells.

Animals↗