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Biomedical subjects

Y Kimoto

Publications and source records attributed to Y Kimoto.

At least 91 records · Page 5Linked to original sources
PubMed1978

Afferent fiber connections from lower brain stem to hypothalamus studied by the horseradish peroxidase method with special reference to noradrenaline innervation.

Attempts were made to determine the afferent projections to the anterior hypothalamus including the preoptic area from the lower brain stem by means of the horseradish peroxidase method combined with monoamine oxidase staining to identify noradrenaline (NA) neurons. In addition to this technique, a histofluorescence analysis was performed. NA fibers in the medial part of the anterior hypothalamus were mainly supplied by A1 and A2 NA neuron groups, while the lateral part and periventricular zone received NA terminals from both pontine and medulla oblongata NA neuron groups. Furthermore, the present study indicated that there were direct projections to the anterior hypothalamus from non-noradrenergic neurons in the lower brain stem: nuclei raphe dorsalis, centralis superior, cells in the mesencephalic and pontine central gray matter, nuclei parabrachialis lateralis and medialis, cells around fasciculus longitudinalis medialis.

Afferent Pathways↗
PubMed1978

Organization and projections of the neurons in the dorsal tegmental area of the rat.

The organization and projection of the neurons in the dorsal tegmental area at the locus coeruleus level of the rat have been investigated by means of the horseradish peroxidase (HRP) method, combined with monoamine oxidase staining to identify the noradrenaline (NA) neurons. Judging from the anatomical aspects elucidated in this study, this area is composed of various different cell groups. Although the functional role of these neuronal groups remains to be determined, the present study strongly suggests that the dorsal tegmental area participates in important regulation of several brain functions.

Animals↗
PubMed1978

Afferent fiber connections from the lower brain stem to the rat cerebellum by the horseradish peroxidase method combined with MAO staining, with special reference to noradrenergic neurons.

The origin of cerebellar noradrenaline (NA) was investigated by means of the horseradish peroxidase (HRP) method, combined with the monoamine oxidase (MAO) staining method to identify the NA neurons. The present study gives clear cut evidence that the main source for cerebellar NA in the rat is the locus coeruleus (Lc), both the dorsal and ventral parts which innervate the entire cerebral cortex and hypothalamus respectively. The present study clearly demonstrates the pontine NA neurons such as the nucleus subcoeruleus (sc) NA neurons also send their axons to the cerebellum. The fact that some neurons in the nucleus dorsalis nervi vagi and the nucleus commissuralis were labeled by HRP indicates strong possibility that A2 NA neurons also innervate the cerebellum, though direct proof was lacking in this study. Furthermore, some unknown afferent fibers to the cerebellum were found in this study. These are as follows: (1) non-noradrenergic neurons in the Sc area, (2) nucleus reticularis gigantocellularis and nucleus reticularis parvocellularis, (3) group 1 (of Meessen and Olszewski), (4) a cell group extending to the ventrolateral region of the reticular formation of the pons, (5) nucleus ambiguug, (6) nucleus nervi facialis.

Animals↗
PubMedDate not supplied

Apoptosis as a mechanism of lectin-dependent monocyte-mediated cytotoxicity.

In this study we investigated the mechanisms of cytotoxicity mediated by pokeweed mitogen (PWM)-activated human peripheral blood monocytes. By using DNA electrophoresis and propidium iodide (PI)-DNA staining flow cytometry, we demonstrated that apoptotic cell death of target U937 cells and Raji cells was induced in lectin (PWM)-dependent monocyte-mediated cytotoxicity (LDMC). The LDMC-mediated DNA fragmentation in U937 cells and Raji cells was induced in lectin (PWM)-dependent monocyte mediated cytotoxicity(LDMC). The LDMC-mediated DNA fragmentation in U937 cells was completely inhibited by anti-TNF alpha monoclonal antibody (mAb), but not by the addition of monosaccharide (N-acetylglucosamine, GlcNAc, a sugar specifically recognized by PWM and a lectin-like receptor on monocytes). In contrast, GlcNAc inhibited the DNA fragmentation in Raji cells induced by LDMC which the anti-TNF alpha mAb had no effect. PWM was found to stimulate the production of nitric oxide (NO) from monocytes. The NO-production was enhanced in the presence of target Raji cells, while the enhancement was abolished by the treatment with GlcNAc. By flow cytometry, we found that PWM bound to tumour cells as well as monocytes, and inhibited the expression of HLA-DR antigen on tumour cells. These results suggest that the presence of lectin molecules on the surface of monocytes and tumour cells may bring the two cells together, thus facilitating the induction of apoptosis in target cells by triggering the production of cytolytic factors (TNF and NO) and the modification of target cell surface antigen (HLA-DR).

Acetylglucosamine↗