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Biomedical subjects

Y Katayama

Publications and source records attributed to Y Katayama.

At least 685 records · Page 38Linked to original sources
PubMed1988

[Effect of glycerol administration on experimental cerebral ischemia--Part 1. Studies on lipid peroxides, prostaglandins, brain edema and brain metabolites].

Using two different models of non ischemic and transient cerebral ischemia in SHR, the effect of hyperosmolar solution with intravenous 10% glycerol on serum lipid peroxides, plasma prostaglandins (TXA2, PGI2), brain water content and brain metabolites were studied. Glycerol did not influence the levels of lipid peroxides, plasma prostaglandins and brain water content in the non ischemic rats. In the transient ischemia group, on the other hand, serum lipid peroxides were significantly reduced in the glycerol administrated group. On the study of plasma prostaglandins, there was no difference of TXA2 levels between two groups, but PGI2 levels were significantly increased in the glycerol administrated group. Brain water content was significantly decreased. And on the study of brain metabolites, ATP concentrations remained higher and lactate concentrations were lower in the glycerol administrated group compared with those in the control group. But there was no difference with pyruvate concentrations between two groups, furthermore L/P ratio improved in the glycerol administrated group. Besides the effect on reduction of brain edema as for hyperosmolar solution, glycerol may indicate improvement of ischemic impediments on brain by the action of antioxidation and reinforcement of PGI2.

Animals↗
PubMed1987

Benign osteoblastoma of the temporal bone of an infant.

Benign osteoblastoma is a rare neoplasm of bone tissue most commonly involving the vertebral column and long bones. Occurrence in the calvarium is extremely rare, and no cases in infants have previously been described. We report a case of benign osteoblastoma occurring in the temporal squama of an infant. The clinical features of this type of tumor occurring in the calvarium are briefly discussed.

Age Factors↗
PubMed1987

Meningeal chondrosarcomatous tumor associated with meningocytic differentiation.

Most meningeal chondrosarcomatous tumors have been regarded as identical to similar tumors occurring elsewhere in the body. The neoplasm reported here, however, clearly demonstrated transitions between usual meningiomas and chondrosarcomatous tumors. The present case thus provides evidence in support of Cushing's and Eisenhardt's proposal that some meningeal chondrosarcomatous tumors are derived from meningeal cells and are histogenetically related to meningiomas.

Aged↗
PubMed1987

Solitary hemangioblastoma within the third ventricle.

A case of solitary hemangioblastoma within the third ventricle is presented. This case was not associated with von Hippel-Lindau disease. Only two other cases of hemangioblastoma arising in the third ventricle have been previously reported.

Adult↗
PubMed1987

Angioma of the cerebellopontine cistern simulating acoustic neurinomas.

We report a cryptic angioma associated with a cisternal hematoma confined to the cerebellopontine cistern occurring in a 29-year-old woman. The angioma was related to the acoustic nerve. The cisternal hematoma appeared to have increased in size gradually with repeated hemorrhages from the angioma. The location of the lesion, in conjunction with the gradual size increase of the cisternal hematoma, resulted in the lesion simulating acoustic neurinomas radiologically as well as neurologically.

Adult↗
PubMed1987

The actions of 5-hydroxytryptamine in the rabbit ciliary ganglion.

Intracellular recordings were made from ciliary ganglion neurones of the rabbit in vitro. 5-Hydroxytryptamine (5-HT) produced a rapid depolarization in 67% of the neurones examined. The 5-HT-induced depolarization persisted in solutions containing zero (1-100 microM), or on the amplitude and the duration of the current used for ionophoresis. The 5-HT-induced depolarization was associated with a marked fall of input resistance and reversed in polarity at about -10 mV. The 5-HT-induced response was reversibly abolished in sodium-free solution. A marked desensitization to this action of 5-HT was observed. After the 5-HT depolarization passed off due to desensitization, 5-HT (1 microM-1 mM) reversibly depressed the amplitude of cholinergic excitatory postsynaptic potentials (EPSPs) in 91% of the neurones tested in a dose-dependent manner, whereas 5-HT did not detectably affect the depolarization induced by ionophoresis of acetylcholine (ACh). The frequency of spontaneous miniature EPSPs (in 20 mM potassium solution) was decreased by 5-HT (30-100 microM). These results demonstrate that 5-HT exerts two separate actions in the rabbit parasympathetic ciliary ganglion. One is a depolarization of ganglion cells due to an increase in membrane permeability to sodium and presumably potassium ions; the other is a depression of ACh release from presynaptic fibres. Further pharmacological characteristics of these actions of 5-HT were investigated and discussed.

Animals↗
PubMed1987

Somatosensory evoked potentials from the thalamic sensory relay nucleus (VPL) in humans: correlations with short latency somatosensory evoked potentials recorded at the scalp.

Somatosensory evoked potentials (SEPs) were recorded in humans from an electrode array which was implanted so that at least two electrodes were placed within the nucleus ventralis posterolateralis (VPL) of the thalamus and/or the medial lemniscus (ML) of the midbrain for therapeutic purposes. Several brief positive deflections (e.g., P11, P13, P14, P15, P16) followed by a slow negative component were recorded from the VPL. The sources of these components were differentiated on the basis of their latency, spatial gradient, and correlation with the sensory experience induced by the stimulation of each recording site. The results indicated that SEPs recorded from the VPL included activity volume-conducted from below the ML (P11), activity in ML fibers running through and terminating within the VPL (P13 and P14), activity in thalamocortical radiations originating in and running through the VPL (P15, P16 and following positive components) and postsynaptic local activity (the negative component). The sources of the scalp-recorded SEPs were also analyzed on the basis of the timing and spatial gradients of these components. The results suggested that the scalp P11 was a potential volume-conducted from below the ML, the scalp P13 and P14 were potentials reflecting the activity of ML fibers, the small notches on the ascending slope on N16 may potentially reflect the activity of thalamocortical radiations, and N16 may reflect the sum of local postsynaptic activity occurring in broad areas of the brain-stem and thalamus.

Electroencephalography↗
PubMed1987

Observations on the actions of substance P and [D-Arg1,D-Pro2,D-Trp7,9,Leu11)substance P on single neurons of the guinea pig submucous plexus.

Intracellular recordings were made from neurons of the guinea pig submucosal plexus and the effects of substance P and the substance P analogue [D-Arg1,D-Pro2,D-Trp7,9,Leu11]substance P were examined. Substance P (20-200 nM) depolarized all submucosal neurons; these depolarizations were shown to be due to a decrease in the resting (or "leak") potassium conductance of the membrane. In approximately 50% of the 46 neurons tested, superfusion with [D-Arg1,D-Pro2,D-Trp7,9,Leu11]substance P (0.2-20 microM) produced a dose-dependent membrane hyperpolarization. This hyperpolarization was prevented by the alpha 2-adrenoceptor antagonist idazoxan (300 nM) or by concentrations of cobalt which abolished all spontaneous and evoked synaptic potentials, indicating that it resulted from release of noradrenaline from sympathetic nerve terminals. [D-Arg1,D-Pro2,D-Trp7,9,Leu11]substance P depressed the amplitude of the three synaptic potentials recorded from submucosal neurons; the concentrations that caused 50% of the maximal inhibition of the fast excitatory postsynaptic potential, the inhibitory postsynaptic potential, and slow excitatory postsynaptic potential were 40 microM, 600 nM and 20 microM, respectively. When idazoxan was present, the substance P analogue was less effective in depressing the amplitudes of the fast and slow excitatory synaptic potentials suggesting that much of its presynaptic inhibition also resulted from release of noradrenaline. These results provide evidence that [D-Arg1,D-Pro2,D-Trp7,9,Leu11]substance P releases noradrenaline from sympathetic nerves in the submucosal plexus. One effect of this is a membrane hyperpolarization; another is a presynaptic inhibition of transmitter release. These actions much limit the usefulness of this "substance P antagonist" in efforts to show that synaptic potentials, such as the slow excitatory synaptic potential, are mediated by substance P.

Animals↗
PubMed1987

5-Hydroxytryptamine effects on the somata of bullfrog primary afferent neurons.

Intracellular recordings were made from neurons in the isolated dorsal root ganglia of bullfrogs. 5-Hydroxytryptamine was applied by superfusion and by ionophoresis. The most common response to 5-hydroxytryptamine in C neurons was a membrane hyperpolarization and this was observed in 80% of cells. This was due to an increase in membrane potassium conductance because it reversed its polarity at about -90 mV. It was blocked by removal of calcium or addition of calcium blockers. (+)-Tubocurarine, methysergide, ketanserin, quipazine, picrotoxin, caffeine and ouabain blocked this response. The next most common response in C neurons was a fast depolarization, particularly readily observed when 5-hydroxytryptamine was applied by ionophoresis. Since this response reversed its polarity at about -10 mV and was blocked by removal of sodium, this was due to an increase in membrane conductance to both sodium and potassium ions. This response was reduced by superfusion of acetylcholine and gamma-aminobutyric acid. (+)-Tubocurarine, quipazine, picrotoxin and caffeine blocked the response. A small proportion of C neurons (16%) responded to superfusion of 5-hydroxytryptamine with a slow depolarization accompanied by an increase in input resistance. This response reversed its polarity at about -90 mV and, therefore, is presumed to result from potassium inactivation. It was blocked by methysergide and ketanserin but not by (+)-tubocurarine or quipazine. A few type A neurons (8%) caused a fast and transient depolarization like the fast depolarization of C neurons. About half of the A neurons showed a slow depolarization associated with a fall in input resistance. This slow response was assumed to be due to an increase in membrane conductance to both potassium and calcium ions because the response reversed its polarity at about -65 mV and was sensitive to change in external concentrations of those ions. This slow response was blocked by (+)-tubocurarine, methysergide, ketanserin, picrotoxin, caffeine and ouabain but not by quipazine. The effects of 5-hydroxytryptamine are discussed in relation to the similar actions described on a variety of other vertebrate and invertebrate nerve cells. The findings imply that dorsal root ganglion cells of bullfrogs are sensitive to 5-hydroxytryptamine and causes multiple types of 5-hydroxytryptamine responses.

Animals↗
PubMed1987

Secretion of Bacillus subtilis alpha-amylase in the periplasmic space of Escherichia coli.

The Bacillus subtilis alpha-amylase structural gene (amyE) lacking its own signal peptide coding sequence was joined to the end of the Escherichia coli alkaline phosphatase (phoA) signal peptide coding sequence by using the technique of oligonucleotide-directed site-specific deletion. On induction of the phoA promoter, the B. subtilis alpha-amylase was expressed and almost all the activity was found in the periplasmic space of E. coli. The sequence of the five amino-terminal amino acids of the secreted polypeptide was Glu-Thr-Ala-Asn-Lys-, and thus the fused protein was correctly processed by the E. coli signal peptidase at the end of the phoA signal peptide.

Bacillus subtilis↗
PubMed1987

Ouabain augments calcium-dependent potassium conductance in visceral primary afferent neurones of the rabbit.

1. The effects of ouabain (1 nM-100 microM) on the membrane properties of rabbit visceral primary afferent neurones (nodose ganglion cells) were studied with intracellular recordings and voltage-clamp techniques in vitro. 2. Ouabain (greater than or equal to 1 microM) often produced a membrane hyperpolarization associated with a fall of membrane resistance in type C neurones. The ouabain-induced hyperpolarization reversed in polarity at about -90 mV. These suggest that the ouabain-induced hyperpolarization is due to an increase in potassium conductance. 3. Both the peak amplitude and the duration of the after-hyperpolarization following an action potential were reversibly increased with increasing concentration of ouabain. In tetraethylammonium (TEA, 10-20 mM) and tetrodotoxin (TTX, 1-10 microM), the duration of both the calcium-dependent action potential and the after-hyperpolarization following the action potential was prolonged by ouabain (greater than or equal to 10 nM). 4. A depolarizing command pulse evoked a slow outward current in TEA (10-20 mM) and TTX (1-10 microM). This was increased in amplitude and prolonged in duration by ouabain (100 nM-1 microM). Such augmentation of the slow outward current by ouabain was usually associated with an increase in a slow inward current during the period of the depolarizing command pulse. 5. An outward current produced by the calcium ionophore A23187 was reversibly augmented by ouabain (greater than or equal to 10 nM). 6. An outward current caused by exchanging a potassium-free superfusion solution for one containing 4.7 or 10 mM-potassium was completely abolished by ouabain (greater than or equal to 10 nM). 7. The hyperpolarization elicited by intracellular injection of calcium was reversibly prolonged by either ouabain (1 microM) or caffeine (10 nM). 8. These results suggest that ouabain augments the after-hyperpolarization both by an increase in calcium influx across the cellular membrane and by an increase in intracellular calcium concentration.

Action Potentials↗