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Biomedical subjects

Y Kanno

Publications and source records attributed to Y Kanno.

At least 163 records · Page 9Linked to original sources
PubMed1981

Inhibition of the formation of electrical cell coupling of FL cell by tumor promoters.

The effect of tumor promoters on the formation of electrical cell coupling between human FL cells was investigated by means of a microelectrode technique. In culture medium containing 100 ng/ml of 12-O-tetradecanoyl phorbol-13-acetate (TPA), only 6% of the cells were coupled after 24 hr while more than 90% of the cells were coupled in control medium. The formation of cell coupling was also inhibited by other tumor promoters, phorbol-12,13-didecanoate, ingenol-13, 20-dibenzoate and mezerein, whereas non-promoting derivatives, phorbol and 4 alpha-phorbol-12, 13 didecanoate did not affect cell coupling formation. 7, 12-Dimethylbenz [a] anthracene, benzo [a] pyrene and N-methyl-N'-nitro-N-nitrosoguanidine did not affect the cell coupling. None of the chemicals used changed any electrical properties of the cell membrane, such as membrane potential and membrane resistance, of FL cells. Thus, it appears that TPA and other related tumor promoters inhibit the formation of cell coupling without changing the physiological properties of the membrane significantly.

Amnion↗
PubMed1981

[Estimation of the urinary aldosterone (author's transl)].

The urinary aldosterone (ALD) was measured by aldosterone RIA kit and the following results were obtained. (1) We have known that ten percent solution of bovine serum albumin (BSA) instead of free serum LAD is used as the diluent of the urine in aldosterone RIA kit. (2) The upper limits of free ALD, HCl-ALD and beta-glucuronidase ALD in the urine diluted with 10% BSA were 1.6, 9.0 and 9.5 micrograms/dl respectively. (3) The values of urinary conjugated HCl-ALD and beta-glucuronidase ALD were approximately 3.5 times and 4 times as much as that of free ALD respectively. (4) A good correlation was obtained among the results of three methods (HCl-ALD, beta-glucuronidase ALD and free ALD). (5) No difference was found in the values of the urinary ALD in the healthy subjects and the patients with essential hypertension, kidney diseases and acute liver diseases.

Aldosterone↗
PubMed1979

A scanning and transmission electron microscope study of the premetamorphic papillae: possible chemoreceptive organs in the oral cavity of an anuran tadpole (Rana japonica).

Premetamorphic papillae of an anuran tadpole (Rana japonica) were studied by scanning and transmission electron microscopy. Premetamorphic papillae of several shapes are seen in and around the oral cavities of tadpoles during late larval and early metamorphic stages. These papillae are composed of three parts: the apical cellular part, the underlying connective tissue core and the epithelial covering. In the apical cellular part, two populations of cells are observed: apical and basal cells. The apical cell has a slender cytoplasmic process whose apical surface is exposed in the oral cavity. The basal cell is located at the basal portion of the apical cellular part of the papilla without researching the oral cavity. A long solitary microvillus and a bunch of short microvilli are seen on the apical surface of each apical cell. The apical cells contain dense-cored vesicles of 100 nm diameter and make synaptic contacts at their basal membranes with terminals of nerve fibers. Possible chemoreceptive function of the premetamorphic papilla is discussed.

Animals↗
PubMed1978

Further study on the effect of mersalyl, an organic mercurial, on relaxing response of a molluscan smooth muscle to monoamines.

The effect of mersalyl on the relaxation of catch by various monoamines was studied in the anterior byssal retractor muscle of Mytilus. As has already been reported, mersalyl blocked the relaxing response to indoleamines but not block that to catecholamines. The relaxations in response to catecholamine-related compounds (dopa, octopamine, tyramine, phenylephrine, beta-phenylethylamine and phenylethanolamine) and hexylamine were, however, antagonized more or less effectively with mersalyl. It was suggested that the catecholamine-related compounds and hexylamine can act on relaxing nerve endings to increase neurotransmitter serotonin in the junctional clefts, and mersalyl antagonizes the relaxation in response to these compounds by blocking the serotonin.

Animals↗
PubMed1978

Effects of heavy metal ions on the electrical properties of mucous epithelial cells in the newt stomach.

Zinc ion, Cu2+ and Cd2+ at 10(-4) M or lower concentrations caused a marked decrease in the membrane potential of mucous epithelial cells in the isolated newt stomach without appreciable change in the effective membrane resistance and electrical coupling ratio. Cysteine and acetylpenicillamine antagonized the action of these ions. These ions as well as ouabain blocked the hyperpolarization of membrane due to an increase in intracellular Na+. The effect of the ions on the relationship between external K+-concentration and membrane potential was similar to that of ouabain. Discussion is made on the possibility that the ions inhibit active ion transport by combining with a SH-group of the cell membrane and, thus, bring about a decrease in membrane potential.

Animals↗