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Biomedical subjects

Y Kameda

Publications and source records attributed to Y Kameda.

At least 217 records · Page 12Linked to original sources
PubMed1979

Immunohistochemical study of the medullary thyroid carcinoma with reference to C-thyroglobulin reaction of tumor cells.

Twelve cases of medullary thyroid carcinoma were investigated ty the immunoperoxidase method using anti-calcitonin, anti-C-thyroglobulin (C-Tg, C cell-immunoreactive thyroglobulin) and anti-19S thyroglobulin antisera. Tumor cells as well as normal C cells revealed distinct immunoreaction for C-Tg besides for calcitonin. In contrast to normal C cells, the tumor cells were stained more intensely by anti-Ctg antiserum than by anti-calcitonin antiserum. Furthermore, there occurred several tumors or some areas of tumors which showed strong response to anti-C-Tg antiserum but weak or no response to anti-calcitonin antiserum. Thus, medullary carcinoma cells synthesized far greater amounts of C-Tg than calcitonin. The small follicles were occasionally observed mingled in typical cell solid masses. They stored colloid-like materials which were intensely immunoreactive to C-Tg but nonreactive to calcitonin. The specific immunoreaction patterns to anti-C-Tg and anti-calcitonin antisera were also obtained on the ground materials of the amyloid. On the histogenesis of amyloids of medullary carcinoma, the C-Tg could be the presursor of the fibrillar protein of amyloids and the component of the fibrillar protein also closely related to calcitonin.

Amyloid↗
PubMed1978

Immunohistochemical study of a large molecular fragment of thyroglobulin in parafollicular cells.

Thyroglobulin-like immunoreactivity of the parafollicular cells was studied by an immunoperoxidase bridge technique using antisera against dog thyroglobulin fragments. 1. The dog parafollicular cells were specifically stained by anti-peak I (27S and larger components fraction) antiserum absorbed with peak II (19S fraction). By this method, they were easily distinguishable from the non-reactive follicular cells and colloid droplets. More sensitive staining of the parafollicular cells was possible with anti-peak I'' (larger components fraction) antiserum. The staining reactions indicated that the antigenic material responsible for immunoreactivity of the parafollicular cells was due to larger molecular components of thyroglobulin corresponding to 32S, 37S or greater than 37S, and was not due to either the 19S thyroglobulin or to the 27S iodoprotein. 2. A conspicuous decrease of the immunoreactive material in the parafollicular cells occurred in the dog after both chronically induced hypercalcemia and antithyroid drug treatment. This coincided with movement of secretory granules containing calcitonin as shown by staining with silver impregnation, HCl-basic dye, and lead-hematoxylin. 3. The antisera against larger molecular components of dog thyroglobulin showed a high degree of cross-reactivity to the parafollicular cells of most of the mammalian species investigated; rats, rabbits, hamsters, mice, cats, lions, goats, cows, and human.

Animals↗
PubMed1977

Occurrence of immunoreactive thyroglobulin in the parafollicular cells of dogs.

When the canine thyroid gland was stained by immunofluorescence and immunoperoxidase methods using undiluted thyroglobulin antiserum, a considerably stronger immunoreactivity was revealed in the parafollicular cells than in the colloid droplets and follicular cells. After induced hypercalcemia and antithyroid drug treatment, the immunoreactivity of the parafollicular cells coinciding with the movement of secretory granules containing calcitonin was conspicuously decreased. An application of diluted serum (above 1:10) produced a strong reaction in the colloid.

Antigens↗
PubMed1977

Electron microscopical and immunohistochemical study on parafollicular cell complex with reference to parafollicular cell as a paraneuron.

In mammalian species, parafollicular cells were usually disseminated among the follicles of the thyroid gland; but they occasionally formed fairly large clusters around the parathyroid IV and thymus IV. These cell clusters were called parafollicular cell complexes and were regarded as the remnant of the embryonic ultimobranchial body. The parafollicular cell complex contained follicular cells in addition to parafollicular cells. Colloid-containing follicles also occurred. In order to assess the developmental and functional relationship between follicular cells and parafollicular cells, the parafollicular cell complex in the dog was investigated by means of immunohistochemical method and electron microscopy. Follicular cells in the parafollicular cell complex showed various ultrastructures representing every developmental stage. The cells forming solid clusters without follicles were indistinguishable from the precursor cells of the parafollicular cells. Parafollicular cells occasionally formed narrow colloid-containing follicles. The results of the immunoperoxidase study indicated that the follicular cells in the parafollicular cell complex elaborated 19s thyroglobulin and stored it as colloid droplets in the follicular lumen. The antigenicity of the colloid droplets in the parafollicular cell comples was much stronger than that of the thyroid follicles. Based on these results, the origin of the parafollicular cells was discussed with special reference to the paraneuron concept of FUJITA (1976). The theory that both follicular and parafollicular cells were of endodermal origin seemed valid.

Animals↗