The retention behaviour of conjugated bile acids has been studied in a reversed phase high performance liquid chromatographic (RP-HPLC) system by using the mixture of methanol and aqueous phosphate buffer as the mobile phase. The retentions of the conjugates in RP-HPLC have been found to be mainly controlled by the glycine and taurine groups. The selectivity between five different glycine and taurine conjugated bile acids is a constant in RP-HPLC. This selectivity has been used for peak identification in the practical separation of conjugated bile acids.

Bile↗

PubMedDate not supplied

Free-solution capillary electrophoretic separation of small hydrophilic peptides.

Small hydrophilic peptides differing in only one glycine amino acid group have been successfully separated by free-solution capillary electrophoresis (FSCE) using phosphate buffer at acid pH. The effects of column temperature and operating current on the migration times of these small peptides have been investigated. The results from FSCE have been compared with those from reversed phase high performance liquid chromatography.

Chromatography, High Pressure Liquid↗

PubMedDate not supplied

High performance liquid chromatographic determination of individual bile acids in serum for automatic diagnosis of various liver and biliary diseases.

A reversed phase high performance liquid chromatographic method for the high sensitivity determination of individual bile acids in serum using a C18 column with a ternary solvent system combined with fluorometric techniques using immobilized enzymes is described. A computer-assisted diagnosis system using pattern recognition was developed to assist the clinical diagnosis of various liver and biliary diseases. A total consistency rate of 95% can be reached using this system.

Acetonitriles↗

PubMedDate not supplied

Diagnostic value on signs of subluxation of cervical vertebrae with radiological examination.

OBJECTIVE: To investigate the relationship between radiographic signs of subluxation in the cervical vertebrae and their clinical diagnostic value. DESIGN: Controlled, clinical study. SETTING: Institute of Clinical Anatomy and Biomechanics and NanFang Hospital of the First Military Medical University, Guangzhou, China. SUBJECTS: Eighty-seven subjects with cervicodynia and 21 asymptomatic volunteers. INTERVENTIONS: Radiological signs of subluxation from anteroposterior, lateral, open-mouth and dynamic radiographs of the cervical vertebrae of the subjects were measured and analyzed. MAIN OUTCOME MEASURES: The right and left odontoid lateral mass interspace, divergence of the spinous processes, sign of double contour and position of odontoid process were studied. RESULTS: The bilateral odontoid lateral mass interspaces were asymmetrical in most cases, and the divergence of spinous processes, sign of double contour and position of odontoid process were also common. Cervical vertebrae C5, C4 and C6 showed no special variations. CONCLUSION: There was little evidence to support the contention that signs of subluxation in the cervical vertebrae are of diagnostic significance. Subluxation should be defined in two ways: as a purely roentgenological diagnosis and as a combination of roentgenological signs with clinical signs.

Adolescent↗