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Biomedical subjects

Y Jia

Publications and source records attributed to Y Jia.

At least 109 records · Page 6Linked to original sources
PubMed1996

[Factors affecting survival of hepatic carcinoma patients and their clinical significance].

UNLABELLED: 621 patients with unresectable hepatocellular carcinoma were given more than 2 times of intervention treatment. The correlations between the survival rates, tumor histology, portal tumor thrombus and the method and frequency of intervention treatment were analyzed. RESULTS: (1) The survival rates were higher in cases with single nodular tumor than in those with diffuse-tumors. (2) The survival rates of cases without tumor thrombus in portal system were significantly higher than those with tumor thrombus. However, no correlation was found between the survival rates and the location of thrombus in portal vein. (3) Intraarterial drug infusion combined with lipoidol plus gelatin sponge embolization gave the best therapeutic resalts. (4) How frequent was the intervention would be determined by the patients' general condition and was dependent on the quality of treatment. The cumulative survival rates were greatly affected by the above-mentioned factors. The treatment needs to be individualized.

Adult↗
PubMed1996

[The technique for extracting liposoluble components of Salvia miltiorrniza].

To investigate a reasonable producting technique for extracting the liposoluble components of Salvia miltiorrhiza, we made an orthogonal test on percolate technique of tanshinones. The results show that the concentration of alcohol has a highly remarkable influence on the extracting ratio of extract and tanshinones, where 65% alcohol is much better than higher concentration alcohol in percolate technique of tanshinones. This new technique can be employed to reduce sharply the cost and increase the extracting ratio.

Abietanes↗
PubMed1995

Energy transfer and trapping in photosystem I reaction centers from cyanobacteria.

A mutant strain of the cyanobacterium Synechocystis 6803, TolE4B, was constructed by genetic deletion of the protein that links phycobilisomes to thylakoid membranes and of the CP43 and CP47 proteins of photosystem II (PSII), leaving the photosystem I (PSI) center as the sole chromophore in the photosynthetic membranes. Both intact membrane and detergent-isolated samples of PSI were characterized by time-resolved and steady-state fluorescence methods. A decay component of approximately 25 ps dominates (99% of the amplitude) the fluorescence of the membrane sample. This result indicates that an intermediate lifetime is not associated with the intact membrane preparation and the charge separation in PSI is irreversible. The decay time of the detergent-isolated sample is similar. The 600-nm excited steady-state fluorescence spectrum displays a red fluorescence peak at approximately 703 nm at room temperature. The 450-nm excited steady-state fluorescence spectrum is dominated by a single peak around 700 nm without 680-nm "bulk" fluorescence. The experimental results were compared with several computer simulations. Assuming an antenna size of 130 chlorophyll molecules, an apparent charge separation time of approximately 1 ps is estimated. Alternatively, the kinetics could be modeled on the basis of a two-domain antenna for PSI, consistent with the available structural data, each containing approximately 65 chlorophyll a molecules. If excitation can migrate freely within each domain and communication between domains occurs only close to the reaction center, a charge separation time of 3-4 ps is obtained instead.

Cyanobacteria↗
PubMed1995

A C. elegans mutant screen based on antibody or histochemical staining.

A method has been developed for isolating mutations in Caenorhabditis elegans that alter antibody or histochemical staining patterns. The basis for this method is a new procedure for making C. elegans permeable that does not kill the eggs contained within the uterus of gravid adult hermaphrodites. A mutagenized population of gravid hermaphrodites is made permeable and then stained with either an antibody or a histochemical stain. Animals that stain aberrantly are picked to individual petri plates and the eggs within the uterus of the stained mother hatch and establish a new genetic line. Antibody and histochemical stains are especially useful phenotypes because the staining pattern will usually directly reflect the gene expression pattern of the gene that codes for the antigen or enzyme. This method was used to isolate mutants that alter the expression of a mec-7lacZ fusion gene. Transgenic animals that contained the mec-7lacZ gene integrated into chromosome I were treated with the mutagen ethylmethanesulfonate, allowed to self-fertilize for two generations and then stained with X-gal or antibodies against beta-galactosidase. Gravid animals that stained abnormally were picked to fresh petri plates and their offspring were used to establish new mutant lines.

Animals↗
PubMed1995

Airways of a hyperresponsive rat strain show decreased relaxant responses to sodium nitroprusside.

The aim of the current studies was to investigate the possibility that a decreased relaxant response to nitric oxide (NO) might contribute to strain-related differences in airway responsiveness in the rat. Isolated tracheal rings from hyperresponsive. Fisher rats were confirmed to be more responsive to carbachol [mean effective concentration (EC50) = 2.45 x 10(-7) M] than those from Lewis (EC50 = 3.60 x 10(-7) M, P < 0.03) and ACI (EC50 = 3.85 x 10(-7) M, P < 0.01) rats. Sodium nitroprusside (SNP), a NO donor, caused relaxation of the carbachol (10(-6) M) contracted tracheal rings, but the half-maximal inhibition concentration (IC50) SNP in Fisher rats (5.60 x 10(-6) M) was significantly higher than in Lewis (1.34 x 10(-6) M, P < 0.001) and ACI rats (1.13 x 10(-6) M, P < 0.0005). The inhibitory effect of SNP on airway responsiveness to inhaled methacholine (MCh) in vivo was also less pronounced for Fisher than Lewis rats. SNP induced an accumulation of guanosine 3',5'-cyclic monophosphate (cGMP) in cultured tracheal smooth muscle cells (TSM). Fisher TSM produced less cGMP on exposure to SNP compared with TSM from ACI (P < 0.01) and Lewis (P < 0.0001) rats. A decreased guanylyl cyclase activity may account for the impaired relaxant effect of SNP in Fisher rats.

Animals↗
PubMed1995

[Adoptive immunotherapy of malignant pleural effusion with TIL/rIL2 (tumor-infiltrating lymphocytes/recombinant interleukin 2)].

TIL of 24 patients with malignant pleural effusions were induced and expanded in vitro. The phenotype of lymphocytes IL2R, NK cytotoxicity activity were analysed in fresh and activated TIL. CEA and cytology of malignant pleural effusion were detected before and after treatment with TIL/rIL2. The results indicated: CD4.CD8.IL2R, NK activity increased markedly, CEA level decreased in all of the patients, tumor cells disappeared in some patients. The pleural effusion disappeared in 63%. The total effective rate of the treatment is 79%. No serious toxicity and side effects were found in 1 to 6 months period of following-up after treatment.

Adult↗
PubMed1995

[Determination of primaquine and hepatic targeting neoglycoalbumin-primaquine in whole blood and liver of mouse by normalphase high-performance liquid chromatography].

We have developed a method to estimate primaquine (PQ) and hepatic targeting antimalarial agent neoglycoalbumin-primaquine (NGA PQ) in whole blood and liver of mice by sensitive and selective high-performance liquid chromatography. The primaquine was extracted out with ether from the basified biological samples in the PQ diphosphate group or from the samples which were acidic hydrolyzed then basified in NGA-PQ group. The extracts were evaporated under N2 then dissolved in the mobile phase. A linear chain analogue of primaquine was used as the internal standard. The samples were injected into the normal phase silica column with chloroform: methanol:ammonia solution (86.8:12.5:0.7,V/V/V) as mobile phase and detected at 254 nm. The average recovery of the method was 100.09 +/- 3.46 (mean +/- s)%; the CV of within-day and between-day were 2.7%-6.13% (n = 5) and 8.32%-13.56% (n = 9) respectively; in PQ group and NGA-PQ group, the PQ concentrations ranged from 10-20,000 ng/ml blood or 1g. liver respectively, and they all yielded a good linear relationship with the peak height ratios. No endogeneous interference was found in chromatograms of biological samples.

Animals↗
PubMed1995

[Determination of panaxadiol and panaxatriol in shihu yeguang pills].

The contents of panaxadiol and panaxatriol in Shihu Yeguang Pills manufactured by pharmaceutical works in Beijing, Jinan, Guangzhou and other places were determined by TLC-densitometry. A quantitative method has been developed to serve as a reference to quality control of these pills.

Chromatography, Thin Layer↗
PubMed1995

[The early treatment of unilateral crossbite].

Fifteen unilateral crossbite patients of primary and mixed dentition with mandibular asymmetry were treated by transverse expansion using removable appliance. Posteroanterior cephalometric analysis was utilized before and after treatment. The results suggested that after the correction of unilateral crossbite, the deviation of midsagittal mandibular total lenth decreased significantly. According to this study, early treatment of unilateral crossbite is indicated.

Cephalometry↗
PubMed1994

Clinical observation on needling extrachannel points in treating mental depression.

Totally 20 cases of mental depression were treated by needling extrachannel points with an effective rate of 90%; comparison of these cases with 21 controls treated with amitriptyline, showed no significant difference in effect (P > 0.05). Measured with Hamilton's scale, factors of anxiety somatization, cognitive disturbance, retardation, sleep disturbance and feeling of despair in both the needling and control groups showed obvious decrease in the mean value, and the change in anxiety somatization was markedly significant in the needling group as compared with that in the controls (P < 0.01). Our studies revealed that after 6 weeks of needling the power of slow wave delta decreased while that of the fast wave alpha increased, all significantly different as compared with that before and during the treatment (P < 0.05).

Acupuncture Points↗
PubMed1993

Antimicrobial compounds from Petalostemum purpureum.

EtOH extracts of Petalostemum purpureum demonstrated antimicrobial activity against bacteria and fungi. Bioassay-directed fractionation led to the isolation of petalostemumol [1] as the active constituent. Its structure was determined by a single crystal X-ray diffraction analysis. A minor component designated petalostemumol G [3] is believed to be an artifact of the isolation procedure. Its structure was confirmed by a single crystal X-ray analysis of its pentamethylether 2. A number of derivatives of 1 and 3, including the Me- and benzylethers and acetates, were prepared.

Acetylation↗
PubMed1993

MHC-independent presentation of mycobacteria to human gamma delta T cells.

The majority of human peripheral gamma delta T cells express antigen receptors using the V gamma 9 and V delta 2 gene products. Cells of this subset have been previously shown to uniformly recognize mycobacteria regardless of their V-(D)-J junctional sequences in an MHC-unrestricted manner. This reactivity superficially resembles activation of alpha beta cells by bacterial superantigens, which are thought to be presented by monomorphic regions of MHC class II molecules. It is not known whether presentation of the mycobacterial antigen to V gamma 9/V delta 2 T cells is also mediated by class II MHC molecules. In order to examine the similarity between presentation of bacterial superantigens to alpha beta T cells and the presentation of mycobacteria to gamma delta T cells we have studied the role of class II MHC molecules in presentation of the mycobacterial antigen AP-MT to V gamma 9/V delta 2 clones. Activation of gamma delta T cells by AP-MT required direct contact with antigen presenting cells, indicating that an interaction with cell surface molecules on antigen presenting cells is required. Class II MHC molecules were neither sufficient nor necessary for effective presentation of AP-MT to the gamma delta T cells, as transfectants expressing class II MHC molecules were unable to present, whereas cell lines lacking expression of MHC class II molecules could present this mycobacterial antigen.(ABSTRACT TRUNCATED AT 250 WORDS)

Animals↗
PubMed1992

Simulations of the temperature dependence of energy transfer in the PSI core antenna.

In order to understand the organization of the PSI core antenna and to interpret results obtained from studies of the temperature and wavelength dependence of energy transfer and trapping in the PSI particles, we have constructed a model for PSI in which spectral heterogeneity is considered via a self-consistent approach based on Forster transport. The temperature dependence of the absorption and emission spectra of the individual Chl molecules in the protein matrix is calculated based on a model Hamiltonian which includes a phonon contribution. Time and wavelength resolved kinetics of PSI at different temperatures are investigated by means of two-dimensional lattice models. We conclude that wavelength-dependent fluorescence decay kinetics result only when two or more bottlenecks exist in the energy transfer and trapping process. A single trap or several pseudo-traps with spectrally identical environments do not lead to wavelength dependent decays. Simple funnel arrangements of the spectral types can be ruled out. At least one pigment with energy lower than the photochemical trap located close to the reaction center is required to produce the trends of the fluorescence lifetimes observed experimentally. The remainder of the core antenna is consistent with a random arrangement of spectral types.

Bacterial Proteins↗
PubMed1992

Energy transfer and trapping in the photosystem I core antenna. A temperature study.

The fluorescence decay kinetics of the photosystem I-only mutant strain of Chlamydomonas reinhardtii, A4d, are used to study energy transfer and structural organization in photosystem I (PSI). Time-resolved measurements over a wide temperature range (36-295 K) have been made both on cells containing approximately 65 core chl a/P700 and an additional 60-70 chl a + b from LHC proteins and on PSI particles containing 40-50 chl a/P700. In each case, the fluorescence decay kinetics is dominated by a short component, tau 1 which is largely attributed to the lifetime of the excitations in the core complex. The results are discussed in terms of simulations of the temperature dependence of tau 1 in model systems. Spectral inhomogeneity and the temperature dependence of the spectral lineshapes are included explicitly in the simulations. Various kinds of antenna arrangements are modeled with and without the inclusion of pigments with lower absorption energies than the trap (red pigments). We conclude that funnel arrangements are not consistent with our measurements. A random model that includes one or two red pigments placed close to the trap shows temperature and wavelength dependence similar to that observed experimentally. A comparison of the temperature dependence of tau 1 for cells and PSI particles is included.

Animals↗
PubMed1992

[Inhibitory effects of the extract of pilose antler on monoamine oxidase in aged mice].

It was demonstrated that the water extract of Pilose Antler (WEPA) showed a higher inhibitory effect on MAO-B activities in the liver and brain tissues of aged mice, but nearly no effect on NAO-A. WEPA could significantly increase the contents of 5-HT, NE and DA in the brain tissues of aged mice. In vitro experiments revealed that the inhibition of WEPA on MAO-B was competitive, but on MAO-A was of mixed-type.

Aging↗
PubMed1992

[Thin layer chromatography for identification of 9 species of traditional Chinese medicine in baihe gujin tang granules].

Baihe Gujin Tang granules are mainly composed of nine species of traditional Chinese medicine and other admixtures, so the composition is very complex. In order to confirm the presence of each traditional Chinese medicine in the preparation, a thin layer chromatographic method was developed successfully for analysis of Scrophulariae, Platycodon, Paeoniae and Glycyrrhizae.

Chromatography, Thin Layer↗