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Biomedical subjects

Y Isayama

Publications and source records attributed to Y Isayama.

At least 91 records · Page 5Linked to original sources
PubMed1977

Isolation of encapsulated strains of Staphylococcus aureus from bovine mastitis milk.

Among 1598 strains of Staphylococcus aureus isolated from bovine mastitis milk samples, 19 exhibited a diffuse-type growth using the serum-soft agar (SSA) technique. By the application of a capsular typing method using the SSA technique, they could be separated into five different serological types. Mouse virulence of individual strains varied significantly. Two strains showed high mouse virulence but the majority showed relatively low virulence or were avirulent. The avirulent strains and those of low virulence were capable of absorbing protective antibodies from rabbit antisera to type strains of human encapsulated S aureus. These results suggested that encapsulated strains of S aureus may play a part in the pathogenesis of bovine mastitis.

Animals↗
PubMed1975

Peculiar secretory IgA system identified in chickens. II. Identification and distribution of free secretory component and immunoglobulins of IgA, IgM, and IgG in chicken external secretions.

A homologue of a free secretory component (SC) was identified in chicken intestinal secretion by criteria based on its antigenic relationship with intestinal secretory IgA (SIgA), molecular size, sugar content, and electrophoretic mobility, as well as its elution characteristic from ion-exchange chromatography. SC was obtained in a form free from IgA from the intestinal secretion by salting out and DEAE chromatography, followed by density ultracentrifuguation or Sephadex G-200 gel-filtration. However, the free SC revealed some antigenic deficiency when compared to bound SC of intestinal SIgA and showed a failure of binding to serum-type-polymeric IgA of biliary IgA in vitro. Several kinds of chicken external secretions were examined for detection of SC and immunoglobulin classes of IgG, IgA, and IgM. In spite of the wide distribution of immunoglobulins in the external secretions, SC antigen could be detected only in intestinal secretion. Most IgA in the secretions had a molecular structure of a tetramer of serum-type IgA, lacking in SC and having 17S to 18.5S and 600,000 to 700,000 daltons. On the other hand, IgA in the intestinal secretion showed close similarity to the mammalian SIgA, associated with SC and having 11.2S and 350,000 daltons. Presence of antibody activity in the intestinal IgA to avian reovirus was confirmed by plaque reduction tests.

Animals↗