Biomedical subjects

Y Inoue

Publications and source records attributed to Y Inoue.

At least 811 records · Page 45Linked to original sources

PubMed1997

Classification system of complications in neuroleptic malignant syndrome.

Our group treated 13 cases of neuroleptic malignant syndrome (NMS) over a period of 8 years. Based on the clinical severity of complications, the cases were classified into three types: mild, with no complications; moderate, with only respiratory disturbance; and severe, with respiratory disturbance and renal failure. The major complications affecting the prognosis of NMS are respiratory disturbance and renal failure. Renal failure is also associated with the occurrence of disseminated intravascular coagulation and rhabdomyolysis. The proposed classification system for NMS patients is useful in selecting the appropriate therapeutic strategy for this disorder. The clinical data were analyzed to determine the factors in the process of deterioration in NMS.

Adolescent↗

PubMed1997

[Codes and names of clinical laboratory tests and shared interlaboratory databases].

Physicians need to combine and compare laboratory data for one patient results from many laboratories and hospital officers and policy makers also need to merge them form the view point of public health. As there are many different codes used by different sources for the same test, it is impossible to transmit results using electronic massage standards. This problem would not exist if all laboratories used the same universal set of test identifiers. Public use sets of codes and names of clinical laboratory tests are available. We have to select and use them properly. We proposed a project to have a systematizing guideline for laboratory systems not for rationalization of laboratory works but for sharing database to assess appropriate laboratory use.

Clinical Laboratory Information Systems↗

PubMed1997

Clinical, morphologic, and biochemical characteristics of Chediak-Higashi syndrome in fifty-six Japanese black cattle.

OBJECTIVE: To characterize Chediak-Higashi syndrome (C-HS) in Japanese Black cattle. ANIMALS: 56 of 200 cattle with a bleeding disorder and giant granules in leukocytes. PROCEDURE: Clinical observation, CBC, hemostatic screening test, platelet aggregometry, electron microscopy, platelet constituent analysis, and ophthalmoscopic examination were done. RESULTS: Affected Japanese Black cattle had increased bleeding tendency and abnormal granules in their leukocytes. Susceptibility to infection was not increased. Cutaneous albinism was evident in 6 new-born calves, but not in most affected cattle. In all affected cattle, the tapetal fundus was pale and the nontapetal fundus was almost devoid of pigment. By electron microscopy, a remarkable decrease in the number of dense granules in platelets was observed. Functionally, collagen-induced platelet aggregation was markedly reduced. CONCLUSIONS: This bleeding disorder was diagnosed as C-HS. With regard to susceptibility to infection, albinism, and mortality, clinical manifestations of C-HS in Japanese Black cattle were moderate, compared with C-HS in human beings and Hereford cattle. CLINICAL RELEVANCE: Because an autosomal recessive mode of inheritance was documented and recessive homozygotes could be easily detected, C-HS in Japanese Black cattle can be controlled.

Adenosine Diphosphate↗

PubMed1997

[Congenital mid-ureteral stricture: report of a case].

A rare case of congenital mid-ureteral stricture is reported. A 17-year-old woman was admitted with sudden onset of right flank pain. Intravenous urography demonstrated bilateral small renal calculi, left hydronephrosis and a stricture of the left ureter at the level of the pelvic brim. The diagnosis was determined as congenital mid-ureteral stricture because the ureter tapered smoothly from 25 mm to 5 mm in diameter at the stenotic site. She was successfully treated by partial ureterectomy with end-to-end anastomosis. Histopathologically, no dysplasia of muscular layer was recognized.

Adolescent↗

PubMed1997

Metabolism of technetium-99m-L,L-ethyl cysteinate dimer in rat and cynomolgus monkey tissue.

UNLABELLED: Technetium-99m-L,L-ethyl cysteinate dimer (99mTc-ECD) is thought to be hydrolyzed in the brain by an enzyme and to be trapped as a hydrophilic product. We investigated the characteristics of the enzymatic system that metabolizes 99mTc-ECD. METHODS: In 50 mM phosphate buffer (pH 7.4), 99mTc-ECD was incubated with various concentrations of homogenates of rat tissues (blood, liver and brain) or cynomolgus monkey tissues (blood, liver, cerebral gray matter, cerebral white matter and cerebellar gray matter), and the metabolic rates were assessed. Inhibition studies were performed using diisopropyl fluorophosphate, eserine and p-chloromercuribenzoate as inhibitors. The metabolic rates in the brain homogenates of rat and monkey were measured at various levels of pH, ranging from 6.6 to 7.6. Technetium-99m-L,L-ethyl cysteinate dimer metabolism was also examined in the presence of purified enzymes. RESULTS: In both species, the metabolic rate was high in liver tissue, intermediate in brain tissue and low in blood. The rate in cerebral gray matter of cynomolgus monkey was higher than those in rat brain, monkey cerebral white matter and monkey cerebellar gray matter. All substances used as inhibitors depressed 99mTc-ECD metabolism, and the response was different among tissues. Reduction in pH induced slight decreases in metabolic rate. Hydrophilic conversion of 99mTc-ECD was observed after incubation with porcine liver carboxylesterase. CONCLUSION: These results support the hypothesis that the hydrophilic conversion of 99mTc-ECD is mediated by enzymes. It is also suggested that various enzymes catalyze the hydrolysis of 99mTc-ECD and that the enzymatic system that metabolizes 99mTc-ECD is different between tissues and between species.

Acetylcholinesterase↗

PubMed1997

Asialoglycoprotein receptor and hepatic blood flow using technetium-99m-DTPA-galactosyl human serum albumin.

UNLABELLED: Asialoglycoprotein receptor (ASGP-R) amount and hepatic blood flow were quantitatively measured by using a newly developed kinetic model of 99mTc-labeled diethylenetriaminepentaacetic acid-galactosyl human serum albumin (99mTc-GSA) in which receptor-mediated endocytosis and receptor recycling were considered. METHODS: Five healthy volunteers were intravenously injected 3-mg and 9-mg 99mTc-GSA doses. The absolute amounts of 99mTc-GSA in the liver and extrahepatic blood were estimated from the time-activity curves for the liver, heart and lung. The metabolic process was represented by five differential equations with 10 parameters as variables. To estimate total receptor amount (Rtotal), hepatic plasma flow (Q) and hepatic plasma volume (Vh), other parameters were fixed and estimated by analyzing their data with the least-squares method. Nineteen patients with liver diseases were given a 3-mg dose, and the data were analyzed to estimate Rtotal, Q and Vh. RESULTS: The values of the fixed parameters were estimated as follows: dissociation constant, 0.032 microM; rate constant for internalization, 0.604 min(-1); and ratio of surface receptors to total receptors, 6.1%. The fitted liver uptake curve corresponded well to the measured data. The simulated liver uptake curve was significantly influenced by Rtotal and Q in cases with normal receptor amounts. Analysis in patients with normal livers, chronic hepatitis and liver cirrhosis showed statistically significant differences in their Rtotal values, but not in their Q or Vh values. The s.e. values of Rtotal, Q and Vh for normal livers were small, and the s.e. values of Q and Vh were high for cirrhotic livers. CONCLUSION: This method is useful for measuring ASGP-R amount and hepatic blood flow simultaneously based on dynamic images, without the need for blood sampling, and reflects the cellular transport of asialoglycoproteins and the ASGP-R recycling mechanism.

Adult↗

PubMed1997

[TNM classification of malignant melanoma and non-melanoma skin cancer].

We abide by a rule of the UICC system of cutaneous malignant melanoma and non-melanoma skin cancer. Assignment of pT stage for melanoma requires accurate measurement of pathological findings. On the other hand, T classification of SCC is decided only by measurement of size. We introduced the UICC TNM staging systems and compared the 5-year survival rate of our cases with the statistical data of the Japanese Society for Skin Cancer.

Basal Cell Carcinoma↗

PubMed1997

[Hepatocyte].

Explore the source record for details and available documents.

Amino Acids↗

PubMed1997

[Current status and problems of home parenteral nutrition for terminal cancer patients from the viewpoint of indication].

Since June 1993, we have been performing home parenteral nutrition (HPN) for end-stage cancer patients. We studied how many patients could be treated with HPN, why they were not able to be treated with HPN in the terminal stage cancer patients, 158 cases, who admitted and died in our ward from June 1993 to April 1997. Eighty-six patients (54.4%) were considered to not be indicated for HPN, due to general weakness (28 cases), dyspnea (17 cases), need for medical care other than HPN (34 cases), and inability to understand this treatment (1 case). Fifty-six patients (35.4%) were considered to have been able to be treated with HPN and could have had returned home. Fifteen patients (26.8%) were given HPN. Four cases wanted to stay in the hospital after they knew the truth and the prognosis of their disease. Another four patients became weak while preparing for HPN and could not go home. Eighty percent of patients could not to be treated with HPN because of the factors of their family. Eight patients had no family to cared for them. Twelve patients were refused to the truth of their disease. The families of 13 cases refused to accept and care for them in their home. We may conclude that co-operation among patients, their family and the medical staff is the most important factor in providing HPN for end-stage cancer patients.

Adult↗

PubMed1997

[For terminal cancer patient inserted with four tubes to live at home--from discussion with family].

The patient was an 86-year-old man with mild dementia, and a urinary bladder catheter already inserted for benign prostata hypertrophy. At this time he had jaundice due to duodenal papillary cancer and was inserted with a percutaneous transhepatic bile drainage tube (PTBD). Additionally, due to an obstruction of his duodenum, he received a percutaneous endoscopic gastrostomy tube (PEG) for suction of gastric juice and intravenous hyperalimentation catheter (IVH) for nutrition. He always wished to return home. The medical staff and his family discussed this many times to realize his hope. We his daughter-in-law how to handle the 4 tubes. When she learned how, he went home and was on the visiting care system. But only one week after, despite his stability, his son, who was living apart got drunk and sent him back to the hospital. We tried to persuade them, including all family members living apart. At last he could return home again, and one week after died quietly at home. We learn from this case that it is important and very difficult for a family to accept a terminal cancer patient at home. It is necessary to advice them from two stand-points, the medical techniques and the mental aspects.

Aged↗

PubMed1997

Anti-inflammatory cytokine levels in patients with septic shock.

We measured the plasma levels of anti-inflammatory cytokines, including interleukin 1 receptor antagonist (IL-1ra), IL-4 and IL-10; inflammatory cytokines, including IL-2, IL-6, IL-8 and tumor necrosis factor receptor I and II (TNFR I and TNFR II); and endotoxin in 11 patients with septic shock associated with gram-negative bacteria and 12 patients with sepsis not associated with shock. The plasma levels of IL-1ra and IL-10 were elevated in the septic shock group compared with the sepsis group. TNFR I and TNFR II levels tend to be higher in the septic shock group. The plasma level of TRNF-alpha was significantly correlated with levels of IL-1ra, IL-4, IL-10, TNFR I, and TNFR II. The elevated levels of the anti-inflammatory cytokines, TNFR I, and TNFR II, appeared to reflect an attempt to suppress the shock syndrome.

Adult↗

PubMed1997

[Localized polyarteritis nodosa presenting as epididymal mass: a case report].

A 31-year-old man was admitted with a mass in the left scrotum. On palpation, the mass was elastic hard and hen's egg-sized. It was impossible to discriminate between testis and epididymis. Left high orchiectomy was performed under the diagnosis of left testicular tumor. Histopathologically, distinct arteritis was demonstrated in the epididymis. Since the patient showed no evidence of systemic disease or serological abnormalities, he was diagnosed to have localized polyarteritis nodosa.

Adult↗

PubMed1997

Specific interaction between 5'-aminonucleosides and borates and influence of borates on nucleoside conformations.

The unique conformation and orientational change of 5'-amino-5'-deoxyuridine in Kolthoff's buffer is discussed. From CD and NOE spectra, anomalous syn orientation of Urd-5'-NH2 might be caused by specific interaction between borates and Urd-5'-NH2, which promote the formation of hydrogen bonding between 2-carbonyl oxygen and hydrogen of 5'-amino group. This borates induced syn orientation of Urd-5'-NH2 could be applied to external stimulation control of target RNA/DNA hybridization.

Borates↗

PubMed1996

Resonance Raman evidence that photodissociation of nitric oxide from the non-heme iron center activates nitrile hydratase from Rhodococcus sp. N-771.

Nitrile hydratase (NHase) from Rhodococcus sp. N-771, which contains a non-heme iron center in the catalytic site, has been known to be activated by light illumination. Recently, endogenous nitric oxide (NO) was found in this enzyme by FTIR spectroscopy [Noguchi et al. (1995) FEBS Lett. 358, 9-12]. In order to directly detect the bonding between NO and the iron atom and the reaction of NO upon photoactivation, resonance Raman spectra of the NHase were measured with 413 nm excitation at 85 K. Two prominent bands at 592 and 570 cm-1 were observed in the inactive from, and both of them were completely lost upon photoactivation. Upon subsequent introduction of 15NO, the active NHase was converted to the inactive form again and the above two bands were restored with downshifts by 10 and 12 cm-1, respectively. Also, the excitation profiles of these bands in the 350-500 nm region mostly followed the absorption spectrum arising from the iron center. From these isotopic shifts and the excitation profiles, the two Raman bands were assigned to the Fe-NO stretching and bending vibrations that are probably coupled with each other. The results provided solid evidence that NO is bound to the non-heme iron in the inactive NHase and its photodissociation activates the enzyme.

Binding Sites↗

PubMed1996

Characterization of the major core structures of the alpha2-->8-linked polysialic acid-containing glycan chains present in neural cell adhesion molecule in embryonic chick brains.

To gain more insight into the possible functional significance of the core glycan chain(s) on which polysialylation takes place in polysialic acid (poly-Sia)-containing glycoproteins, the structure of the core glycans in the embryonic form of chick brain neural cell adhesion molecule (N-CAM) were examined using chemical and instrumental techniques. The following new structural features, which had not been reported by the early pioneering study by Finne (Finne, J. (1982) J. Biol. Chem. 257, 11966-11970), were revealed (Structure I). (i) Two distinct types of multiantennary N-linked glycans, i.e. tri- and tetra-antennary structures, are present; (ii) an alpha1-->6-linked fucosyl residue is attached to the proximal GlcNAc residue of the di-N-acetylchitobiosyl unit; (iii) that the action of GlcNAc-transferase V, which catalyzes the attachment of the beta-(1-->6)-linked GlcNAc residue on the (1-->6)-alpha-linked mannose (Man) arm, appears to be essential for polysialylation to occur on the core glycan chain is suggested by the fact that the Man residue alpha1-->6-linked to the beta-linked Man residue is invariably 2,6-di-O-substituted by the GlcNAc residue; (iv) both type 1 (Galbeta1-->3GlcNAc) and type 2 (Galbeta1-->4 GlcNAc) sequences are present in the peripheral portion of the core glycan structure. An extended form of the type 2 chain, i.e. Galbeta1-->4GlcNAcbeta1-->3Galbeta1-->4GlcNAc, is also expressed on the (1-->3)- and (1-->6)-alpha-linked Man arms; (v) on average about 1.4 mol of sulfate is attached to the type 2 N-acetyllactosamine chain(s), where in the extended form the sulfate group is probably substituted at the O-3 position of the outmost GlcNAc residue, i.e. Galbeta1-->4(HSO3-->3)GlcNAcbeta1-->3Galbeta1--> 4GlcNAcbeta1-->Man. It is possible that the unusual structural features identified in this study might play a role in the initiation of polysialylation and our data should facilitate future research regarding the signals that control polysialylation.

Acetylation↗

PubMed1996

Electronic and vibrational structure of the radical cation of P840 in the putative homodimeric reaction center from Chlorobium tepidum as studied by FTIR spectroscopy.

Light-induced FTIR difference spectra of P840 upon its oxidation (P840+/P840) have been measured with the reaction center complex from the green sulfur bacterium Chlorobium tepidum. A broad band centered near 2500 cm-1 was observed in P840+, which is comparable to the band near 2600 cm-1 previously observed in P870+ of purple bacteria and assigned to the electronic transition in the bacteriochlorophyll a (BChla) dimer (Breton et al. (1992) Biochemistry 31, 7503-7510]. The intensity of this electronic band found in P840+ was about the same as that in P870+. The P840+ spectrum also showed several intensified vibrational modes, which are characteristic of the P870+ spectrum as well. These similar features of the electronic transition and the intensified lines indicate that P840+ is a BChla dimer whose electronic structure is similar to P870+. Based on the previous theoretical works, the possibility that P840+ has an asymmetric structure as P870+ was suggested. Also, two strong positive bands at 1707 and 1694 cm-1 probably assigned to the keto C9 = O stretching modes of P840+ were observed in the P840+/P840 spectrum. Three different interpretations are possible for the presence of the two C9 = O bands: (i) P840+ is an asymmetric dimer cation. (ii) P840+ has a symmetric structure, and the time constant of positive charge exchange between the two BChla molecules coincides with that of IR spectroscopy (10-13 s). (iii) The electric field produced by the positive charge on P840+ affects the C9 = O frequency of the neutral BChla in P840+ itself (when the charge exchange time is slower than the time scale of 10-13 s) or of a BChla in the close proximity of P840+. The negative bands at 1734 and 1684 cm-1 were assigned to the ester C10 = O and the keto C9 = O of neutral P840, respectively, both of which are free from hydrogen bonding. These results and interpretations regarding the structural symmetry and the molecular interactions of P840 and P840+ are discussed in the framework of the "homodimeric" reaction center of green sulfur bacteria.

Chlorobi↗

PubMed1996

Separation of naphthalene and flavone derivatives by micellar electrokinetic chromatography with double- and triple-chain surfactants.

Three surfactants, p-bis(2-dodecyloxymethyl-3-oxa-6-sodiosulfonatohexyloxy)benz ene (BDSB) having two sulfonate groups and two lipophilic chains, disodium 10-dodecanoyl-5,15-bis(dodecyloxymethyl)-10-aza-4,7,13,16-tetra oxa-1,19-nonadecanedisulfonate (DDBTN) having two sulfonate groups and three lipophilic chains and disodium 4,11-bis(dodecyloxymethyl)-3,6,9,12-tetraoxa-1,14-tetrade canedionate (DBTT) having two carboxylate groups and two lipophilic chains, were used in micellar electrokinetic chromatography (MEKC). Eight naphthalene derivatives were baseline separated at 10 mM BDSB or 5 mM DBTT, and five flavone derivatives at 5 mM BDSB, DDBTN or DBTT. The elution order of the naphthalene derivatives in MEKC with BDSB was identical with that with DDBTN. However, this elution order was different from that found with DBTT. In the case of the flavone derivatives, BDSB, DDBTN and DBTT produced the identical elution order. These double- and triple-chain surfactants exhibited different selectivity when compared with widely used sodium dodecyl sulfate.

Electrophoresis, Capillary↗

PubMed1996

Importance of catalase in the adaptive response to hydrogen peroxide: analysis of acatalasaemic Saccharomyces cerevisiae.

Controversy about the importance of catalase in the detoxification of H2O2 in human erythrocytes continues. It has been suggested that catalase has no role in the clearance of H2O2 in erythrocytes. In the present study we investigated the role of catalase in the defence mechanism against oxidative stress using Saccharomyces cerevisiae. S. cerevisiae has two catalases, catalase A and catalase T. We constructed a double mutant (acatalasaemic mutant) unable to produce either catalase A or catalase T, and compared it with wild-type and single-mutant cells. The acatalasaemic mutant cells showed a similar growth rate to wild-type cells under non-oxidative stress conditions, and showed a similar susceptibility to H2O2 stress in the exponential growth phase. The acatalasaemic mutant cells at stationary phase were, however, much more sensitive to H2O2 stress than wild-type and single-mutant cells. Moreover, the ability of acatalasaemic and single-mutant cells to show adaptation to 2 mM H2O2 was distinctly inferior to that of wild-type cells. These results suggest that catalase is not essential for yeast cells under normal conditions, but plays an important role in the acquisition of tolerance to oxidative stress in the adaptive response of these cells.

Adaptation, Physiological↗