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Biomedical subjects

Y Iimura

Publications and source records attributed to Y Iimura.

At least 55 records · Page 3Linked to original sources
PubMed1989

[The study of transcatheter arterial chemo-lipiodol administration in liver metastasis of colorectal cancer].

Transcatheter hepatic arterial chemo-lipiodolization (TAC), using totally implantable reservoir, was performed for the treatment of liver metastasis from colorectal cancers in three cases of H1 (metastasis in one lobe only, n = 10), in 3 cases of H2 (a few scattered metastases in both lobes, n = 7), and in 2 cases of H3 (numerous metastases in both lobes, n = 9). We performed TAC in H1 cases after resection of liver tumor. We found a recurrence on lipiodol CT 2 months later in 1 case of H1. The metastatic tumor responded to TAC in two patients of H2 (1 case completely), but no response was observed in H3 cases. The present study suggested the effectiveness of TAC in cases H1 and H2, but further study is needed for cases of H3.

Adult↗
PubMed1986

Mutations in conserved intron sequences affect multiple steps in the yeast splicing pathway, particularly assembly of the spliceosome.

Yeast introns contain three highly conserved sequences which are known to be required for splicing of pre-mRNA. Using in vitro mutagenesis, we have synthesized seven point mutations at five different sites in these signals in the yeast actin intron. The mutant introns were then inserted into each of three constructs, which allowed us to assess the consequences both in vivo and in vitro. In virtually every case, we found the efficiency of splicing to be significantly depressed; mature mRNA levels in vivo ranged from 0 to 47% of wild-type. Surprisingly, the tightest mutations were not necessarily at the sites of nucleolytic cleavage and branch formation; these nucleotides are thus highly preferred, but are not absolutely necessary. Moreover, while particular nucleotides are specifically required for the final step in splicing, i.e. 3' cleavage and exon ligation, the predominant consequence of mutation within the conserved signals appears to be the inhibition of assembly of the splicing complex.

Actins↗
PubMed1981

Thickening of basement membrane of muscle capillary in spontaneously diabetic KK mice.

In order to clarify the relationship between the thickening of muscle capillary basement membrane (MCBM) and diabetes mellitus, an experimental study was carried out using spontaneously diabetic KK mice. Glucose tolerance tests and the measurements of the width of MCBM were performed in KK mice and DD mice at the age of one week through 16 months. The KK mice, in general, revealed a less increase in body weight, compared with the control mice. Impaired glucose tolerance in the KK mice was observed at the age of 2 weeks and remained for 12 months. The width of MCBM increased in both KK and DD mice with the aging process until 6 months. Thereafter, however, the KK mice revealed a significant increase of the width of MCBM. In both KK mice and DD mice, significant correlations were observed between age and the width of MCBM. In contrast, there was no significant correlation between glucose intolerance figured out as the sum of blood glucose levels and the width of MCBM. The present study suggests that the thickening of MCBM in the KK mice may occur as an aging phenomenon on the one hand and may develop as the consequence of long-term carbohydrate derangement based on genetic disposition on the other hand.

Aging↗
PubMed1979

A new method for screening for hyperammonemia.

A new method for the detection of hyperammonemia, using a kit based on the principle of microdiffusion of ammonia, is described. The method requires only one drop of blood and takes only 15 min to complete. Experiments for recovery and reproducibility were satisfactory, and good correlation was obtained when compared with an enzymatic method for blood ammonia determination. The new method is considered to be useful for routine, low-cost mass-screening of newborn infants for hyperammonemia. It will also be useful for monitoring blood ammonia levels at the bedside in cases with hepatic disease or receiving parenteral nutrition.

Amino Acid Metabolism, Inborn Errors↗