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Biomedical subjects

Y Hu

Publications and source records attributed to Y Hu.

At least 631 records · Page 35Linked to original sources
PubMed1992

[Study of 315 pedigrees with idiopathic mental retardation by segregation analysis].

315 pedigrees with idiopathic mental retardation (MR) have been studied by complex segregation analysis. The results show that there is no major gene effect in the mild MR, but in the medium and severe MR there are major gene effect. The pattern of inheritance of the medium MR may be codominant and the penetrance of the dominant gene is 0.91. The mode of inheritance of the severe MR may be recessive and probably without sporadic cases.

Adolescent↗
PubMed1992

[Effects of artificial cultured Panax notoginseng cell on cardiovascular system].

Alcoholic extract from artificial cultured Panax notoginseng cell (SCC) ip has been found conducive to increasing the resistance of mice to anoxia. In vitro it helps to increase the outflow of coronary vessels, decrease the heart rate, inhibit the constriction of aortic strip stimulated by nor-epinephrine and relax spasmodic constriction of ileum smooth muscles markedly. SCC powder suspension on po administration can contract bleeding and coagulation time. The pharmacologic activities of SCC are similar to those of crude Panax notoginseng.

Animals↗
PubMed1992

[The toxicity of herbicide Asulam].

We conducted a series of toxicity tests and short-term mutagenic assays of Asulam and 40% (W/V) sodium Asulam. It was found that the LD50 of Asulam with acute oral toxicity was 30000 mg/kg for mice, and the LD50 of sodium Asulam for mice and rats were equal (8250 mg/kg). The cumulative coefficient of sodium Asulam in Wistar rats was 9.42. None died from sodium Asulam absorbed via skin. Negative results were obtained in Ames test, Bacillus subtilis repair test and the micronucleus test. There was no significant difference between the control group and the treated groups in the chromosomal aberration rates of spermatogonia and primary spermatocytes of mice testis. The results indicated that Asulam should be regarded as a substance of low toxicity and low accumulation. No mutagenicity was observed in our experiment.

Animals↗
PubMed1992

Effects of l-stepholidine on synaptosomal Ca(2+)-ATPase and subcellular calmodulin in rat striatum.

Our results showed that l-stepholidine (l-SPD) inhibited basal Ca(2+)-ATPase activity in rat striatal synaptosomes with an IC50 of 31.5 mumol.L-1, suggesting its interaction with Ca2+ transport. l-SPD inhibited also calmodulin (CaM)-activated basal Ca(2+)-ATPase in a concentration-dependent manner. A complete reversal of CaM activation of Ca(2+)-ATPase was observed with l-SPD 10 mumol.L-1. The activity of synaptosomal Ca(2+)-ATPase and membrane-bound CaM level were decreased in haloperidol (1 mg.kg-1.d-1, ip) and l-SPD (5, 10, and 30 mg.kg-1.d-1, ip) treated rats for 7 and 14 d, respectively. But the activity of Ca(2+)-ATPase and membrane CaM level were increased after treatment with the same of doses haloperidol and l-SPD for 21 d. During the treatments with haloperidol and l-SPD cytosolic and nuclear CaM levels were not altered. These results suggest that l-SPD may modulate the release and synthesis of dopamine (DA) and the negative feedback regulation of presynaptic DA receptors by altering Ca2+ and CaM regulating processes in the central dopaminergic nervous system.

Animals↗
PubMed1992

[Purification, characterization and reverse cholesterol transport of human serum apolipoprotein A-IV].

In order to study the function of apoA-IV, human serum apoA-IV was isolated and purified. LDS was isolated with NaBr density gradient ultracentrifugation and then incubated with intralipid according to the method of Weinberg and Scanu. Protein complex containing apoA-IV was obtained. The apo-IV was purified from protein complex by preparative SDS-PAGE, electroelution and removal of SDS. ApoA-IV thus prepared gave a single band on SDS-PAGE with a MW of 46 kD. Amino acid composition and isoelectric focusing pI (5.27 and 5.47) of apoA-IV were similar to those reported in the literature. The effects of apoA-IV and liposome (apoA-IV: DMPC) on cholesterol efflux from hum skin fibroblast were studied. There was no significant difference (P > 0.5) between apoA-IV and control but a very significant difference (P < 0.001) between liposome (apoA-IV:DMPC) and control, suggesting that liposomes (apoA-IV:DMPC) play a role in reverse cholesterol transport. The results may offer a new approach for the prevention and treatment of atherosclerotic coronary heart disease.

Amino Acids↗
PubMed1992

Antagonism of l-stepholidine on D2 receptor-mediated inhibition of synaptosomal adenylate cyclase in rat corpus striatum.

In the presence of Sch 23390 0.1 mumol.L-1, dopamine (DA) inhibited the activity of synaptosomal adenylate cyclase (AC) isolated from rat striatum in a concentration-dependent manner with IC50 value of 2.2 mumol.L-1. The maximal inhibition corresponded to a 51% decrease of basal enzyme activity and was obtained at DA 100 mumol.L-1. The inhibitory effect of DA was reversed by selective D2 receptor antagonist spiperone 10 mumol.L-1. N-0437, a selective D2 DA receptor agonist also inhibited the activity of AC in the manner similar to that of DA. Both the inhibitions induced by DA and N-0437 were antagonized by l-stepholidine (l-SPD). However, in the presence of Sch 23390, l-SPD alone also inhibited the activity of AC by 29% and 33% at the concentrations of 10 and 100 mumol.L-1, respectively. The inhibition of l-SPD on AC activity was significantly antagonized by spiperone. In the presence of Sch 23390, both DA and N-0437 increased the activity of high affinity GTP phosphohydrolase in striatal synaptosomes. The increases of GTP phosphohydrolase activity stimulated by DA and N-0437 were completely reversed by l-SPD 100 mumol.L-1. These results suggest that l-SPD antagonizes or reverses the D2 receptor-mediated inhibition of AC activity through affecting the regulation of Gi (inhibitory guanine nucleotide-dependent protein) on D2 receptor-coupled AC and therefore affects the negative feedback of presynaptic DA receptors. Moreover, the inhibition of AC activity induced by l-SPD alone may provide a useful biochemical index for dual action of l-SPD.

Adenylyl Cyclases↗
PubMed1992

[Tissue-specific expression of the chimeric gene delta KpF in transgenic mice].

The expression of the chimeric gene delta KpF in which the promoter region of delta-crystallin gene was replaced with the long terminal repeats (LTR) of Friend spleen focus forming virus (SFFV) was studied in transgenic mice for approaching the regulatory mechanism of the expression of chicken delta-crystallin gene. The recombinant plasmid (p delta KpF) DNA containing delta KpF gene was microinjected into male pronuclei of fertilized mouse eggs. The injected eggs were implanted into the oviducts of pseudopregnant female mice and allowed to develop to term. Subsequently, 35 mice from those eggs were born. The mice were analyzed at 2 weeks of age with respect to gene integration and expression by the method of DNA blot hybridization and immunoenzymologic assay. DNA-DNA hybridization indicated that the genomic DNA from one of the mice retained the sequences that hybridized strongly with the probe, p delta KpF. Enzyme linked immunosorbent assay (ELISA) showed that delta KpF gene was expressed in the lens, but not in the brain, muscle and liver. These results demonstrate that the essential regulatory elements for tissue-specific expression of chicken delta-crystallin gene reside in the sequences downstream from +677bp.

Animals↗
PubMed1991

Amelogenin post-secretory processing during biomineralization in the postnatal mouse molar tooth.

The primary structures, molecular genetics and biosynthesis of the amelogenin protein of the developing tooth are established, but knowledge of their subsequent post-secretory processing and its relation to enamel biomineralization is fragmentary. Preparations of tooth matrix proteins were isolated from molars (M1) of mice from birth to 15 days and analysed by SDS-PAGE and immunochemical methods. Amelogenin proteins, isolated and partially purified by HPLC, were characterized by amino acid analysis and SDS-PAGE. At birth a 26 kDa amelogenin was present that during subsequent developmental stages generated a series of 20-25 kDa amelogenins differing in apparent size by approximately 1 kDa. Amino acid analyses showed that all these amelogenins have amino-terminal TRAP sequences; analyses for both glycosylation and phosphorylation were negative. It is suggested that these post-secretory amelogenins are generated by a sequence of specific carboxy-terminal cleavages, and that the observed post-secretory processing of amelogenin is functionally linked to the structure of the enamel matrix and the control of crystallite development.

Age Factors↗
PubMed1991

A histological, ultrastructural and immunohistochemical study of superficial temporal arteries and middle meningeal arteries in moyamoya disease.

Pathologic changes in superficial temporal arteries (STA) and middle meningeal arteries (MMA) biopsied from 15 patients with moyamoya disease (MD) who had undergone cerebro-temporal arterio-synangiosis were studied histologically, ultrastructurally and immunohistochemically. The main pathologic features were: proliferation of smooth muscle cells (SMCs) and thickening of the intima, degeneration and destruction of SMCs in the media and intima, and the presence of condensed organelles in necrosed SMCs or the interstitium among SMCs, or both outside and within the elastica interna (EI). The EI had become thin, porous, fragmented and was even absent in some segments. These changes are different from those of other forms of angiopathy, but identical with those at the ends of internal carotid arteries (ICA) reported by us previously, being pathognomonic for MD. These changes in the STA and MMA reveal that MD involves not only the ICA but also the intra- and extracranial branches of external carotid arteries. The medial necrosis of SMCs seems to be the primary injury of the arterial wall in MD. STA tissue blocks from two cases of MD were stained immunohistochemically. By electron microscopy, IgG-, IgM-, and C3-positive granules were observed on the ER of endothelial and intimal cells. Further studies on more cases are needed to determine whether an immunoreaction has occurred in these arteries.

Adolescent↗
PubMed1991

Uterine influences on the formation of subnormal corpora lutea in seasonally anestrous ewes.

The hypothesis that subnormal luteal function after induced ovulation in anestrous ewes was the result of uterine influences exerted during the periovulatory period was tested. Crossbred ewes (n = 27) in seasonal anestrus were induced to ovulate by administration of 12 doses of 250 ng of LHRH at 2-h intervals, followed immediately by a bolus injection of LHRH (250 micrograms; d 0). Ewes were unilaterally hysterectomized on either d -3 (PRELHRH) or 2 (POSTLHRH). Daily blood samples were collected and assayed for progesterone (P4) and 13,14-dihydro-15-keto-prostaglandin F2 alpha (PGFM). All ewes were slaughtered on d 10, and corpora lutea (CL) were collected, weighed, and assayed for concentration of P4. All ewes that ovulated exclusively in the ovary ipsilateral to the remaining uterine horn had a transient increase in plasma P4 of 2 to 3 d (short luteal phase). In ewes with at least one CL in the isolated ovary, elevated plasma P4 was maintained after hysterectomy but was consistently lower (P less than .05) in POSTLHRH ewes than in PRELHRH ewes. Concentrations of PGFM did not differ between treatments. The CL ipsilateral to the remaining uterine horn weighted less (P less than .01) and contained less P4 (P less than .01) than contralateral CL. These data confirm the hypothesis that premature regression of subnormal CL is uterine-dependent in a local fashion. Presence of the uterus during the follicular and(or) early luteal phase inhibited subsequent luteal function in seasonally anestrous ewes.

Anestrus↗
PubMed1991

[Differentiation of Magnolia denudata Desr. flower buds].

The article gives the results of an observation on the differentiation of Magnolia denudata buds. In Huaining County, Anhui Province, differentiation of the buds starts at the beginning of May. By the end of June the differentiation of various parts of the flower is completed. The process takes about 50 days. The differentiation of buds goes on at a fairly fast speed and in a fairly uniform way. Based on the results of the observation, the authors advise that application of fertilizer, especially the top application should not be late. The beginning of March is a very important time for the development of buds in length and size, and for the final harvest as well. The proper time to pick the flower buds comes when they are fully developed before the perianth appears.

Fertilizers↗
PubMed1991

Effects of hyperlipidemic sera and lipoproteins on prostacyclin synthesis by rabbit aortic smooth muscle cells.

The effects of various hyperlipidemic sera and serum high density lipoproteins (HDL) and low density lipoproteins (LDL) on the synthesis of prostacyclin (PGI2) by rabbit aortic smooth muscle cells in culture were studied. Data show that HDL induces a dose-dependent increase in the synthesis of PGI2 in smooth muscle cells. Although LDL at comparable cholesterol concentrations to HDL also results in an increase of PGI2 synthesis in a dose-dependent pattern, the extent of this increase is much less than that induced by HDL. Smooth muscle cells incubated with hyperlipidemic serum and hyperlipidemic + aspirin-treated sera synthesize less PGI2 as compared to normal serum, but cells incubated with hyperlipidemic + 8501-treated serum generate an amount of PGI2 similar to that seen with normal serum. We suggest that 8501 may directly stimulate PGI2 synthetase in smooth muscle cells.

6-Ketoprostaglandin F1 alpha↗
PubMed1991

A study on the catalytic performance of lipase in reverse micelles.

The hydrolysis of olive oil catalyzed by Candida rugosa lipase solubilized in "H2O/AOT/isooctane" reverse micellar solution has been studied. The optimal conditions observed for lipase in reverse micelles were t = 25 degrees C, pH = 7.9, and R = [H2O]/[AOT] = 10. Lipase in reverse micelles is found to be quite stable. For example, after being incubated at 33 degrees C for 12 hours in AOT/isooctane with R = 5.4, the residual activity of lipase remains as high as 89%. No inhibition was observed even though the substrate concentration was increased up to 50% (v/v), and it was inhibited slightly when 40 mM Cu2+, Co2+ or Mn2+ ions were in the water pools.

Candida↗
PubMed1991

Clinical and experimental observations on treatment of peptic ulcer with wei yang an (easing peptic-ulcer) capsule.

The therapeutic effects of peptic ulcer treated with cimetidine and Wei Yang An were compared clinically and experimentally by the double blind method. Results showed that the effects of the two are similar, but the long-term effect of Wei Yang An was better. Wei Yang An was also good for most cimetidine resistant ulcers, with few side effect. Animal experiment showed that Wei Yang An inhibited the ulcers.

Adult↗
PubMed1991

[A case-control study on risk factors of female hypertension in Tianjin City].

A pair matched case-control study was conducted in 301 pairs female patients with essential hypertension and their controls. The data was analysed by the multiple conditional Logistic regression. The results showed the risk factors associated with hypertension were: body weight (OR = 1.17, 95% CI: 1.12-1.23), the family history of hypertension (OR = 2.18, 95% CI: 1.49-3.21) the family income (OR = 0.73, 95% CI: 0.56-0.94), heart rate (OR = 2.30, 95% CI: 1.41-3.76). OR for the age of menarche was significantly different before and after being adjusted by body weight. There were no relationship between the age of menopause, the age of the first birth, parties, oral contraceptive use or smoking status and hypertension. The paper suggested that the difference of prevalence of hypertension in male and female over 45 years old cannot be explained by menopause or the factors on birth in women.

Adult↗