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Biomedical subjects

Y Hosaka

Publications and source records attributed to Y Hosaka.

At least 199 records · Page 11Linked to original sources
PubMed1977

Purification of Sendai virions with glutaraldehyde-treated red blood cells.

The use of glutaraldehyde (GA)-treated chicken red blood cells (RBC) for purification of Sendai virions by adsorption and elution was found to be advantageous over the use of untreated RBC. Biologic and enzymatic activities and structural components of the purified virions were compared with those of the original virions. The purified virions lost glucosamine-labeled non-proteinous materials. These materials did not relate to the expression of infectivity or cell fusion activity of Sendai virus. No evidence of disruption of Sendai virions during interaction with GA-RBC was observed. The use of GA-RBC provides a new method to prepare an unadsorbed virus fraction with a minimum contamination of hemagglutinins. The unadsorbed fraction consisted of Sendai virions with weak hemagglutinating activity and smaller amounts of the gp1(HN) polypeptide and with negligible hemolytic and cell fusion activities. The origin of these particles is discussed.

Adsorption↗
PubMed1972

Solubilization of envelopes of HVJ (Sendai virus) with alkali-emasol treatment and reassembly of envelope particles with removal of the detergent.

The envelopes of HVJ (Sendai virus) virions were solubilized with alkali-Emasol treatment. The solubilized envelope subunit(s) associated with hemagglutination-inhibiting antibody blocking, neuraminidase, and low hemagglutinating (HA) activities had a sedimentation coefficient of 8.8S. Envelope fragment-like structures were assembled from the solubilized subunits after Emasol was removed by gel filtration. These reassembled envelope particles with HA activity had cell-fusion activity as well as hemolytic activity. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis of the reassembled particles revealed that they mainly consisted of two kinds of polypeptides.

Alkalies↗