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Biomedical subjects

Y Hiji

Publications and source records attributed to Y Hiji.

At least 19 recordsLinked to original sources
PubMed2001

Inhibitory effect and mechanism of acarbose combined with gymnemic acid on maltose absorption in rat intestine.

AIM: To compare the combinative and individual effect of acarbose and gymnemic acid (GA) on maltose absorption and hydrolysis in small intestine to determine whether nutrient control in diabetic care can be improved by combination of them. METHODS: The absorption and hydrolysis of maltose were studied by cyclic perfusion of intestinal loops in situ and motility of the intestine was recorded with the intestinal ring in vitro using Wistar rats. RESULTS: The total inhibitory rate of maltose absorption was improved by the combination of GA (0.1g/L-1.0 g/L) and acarbose (0.1 mmol/L-2.0 mmol/L) throughout their effective duration (P <0.05, U test of Mann-Whitney), although the improvement only could be seen at a low dosage during the first hour. With the combination, inhibitory duration of acarbose on maltose absorption was prolonged to 3h and the inhibitory effect onset of GA was fastened to 15 min. GA suppressed the intestinal mobility with a good correlation (r = 0.98) to the inhibitory effect of GA on maltose absorption and the inhibitory effect of 2 mmol/L (high dose) acarbose on maltose hydrolysis was dual modulated by 1g/L GA in vivo indicating that the combined effects involved the functional alteration of intestinal barriers. CONCLUSION: There are augmented effects of acarbose and GA,which involve pre-cellular and paracellular barriers. Diabetic care can be improved by employing the combination.

Acarbose↗
PubMed2001

Inhibitory effect of voglibose and gymnemic acid on maltose absorption in vivo.

AIM: To determine whether diabetic care can be improved by combination of voglibose and gymnemic acid (GA), we compared the combinative and individual effects of voglibose and GA on maltose absorption in small intestine. METHODS: The small intestine 30 cm long from 2 cm caudal ward Treitz's ligament of Wistar rat was used as an in situ loop, which was randomly perfused in recircular mode with maltose (10mmol/L) with or without different dosages of voglibose and/or GA for an hour. To compare the time course, perfusion of 10 mmol/L maltose was repeated four times. Each time continued for 1 hour and separated by 30 minutes rinse. In the first time, lower dosages of GA (0.5g/L) and/or voglibose (2 micromol/L) were contained except control. RESULTS: Absorptive rate of maltose was the lowest in combinative group (P<0.05, ANOVA), for example, the inhibition rate was about 37% during the first hour when 0.5 g/L-GA and 2 micromol/L voglibose with 10 mmol/L maltose were perfused in the loop. The onset time was shortened to 30 minutes and the effective duration was prolonged to 4 hours with the combination; therefore the total amount of maltose absorption during the effective duration was inhibited more significantly than that in the individual administration (P < 0.05, U test of Mann Whitney). The effect of GA on absorptive barriers of the intestine played an important role in the combinative effects. CONCLUSION: There are augmented effects of voglibose and GA. The management of diabetes mellitus can be improved by employing the combination.

Animals↗
PubMed1999

(-)-Epigallocatechin gallate inhibits mos activation-mediated xenopus oocyte maturation induced by progesterone.

(-)-Epigallocatechin gallate (EGCG), one of the constituents of green tea known to have a tumor preventing effect, inhibited maturation of Xenopus laevis oocytes induced by progesterone when this polyphenol was microinjected into oocytes at a final concentration of about 1 mM. Western blot and activity measurement analyses showed that Mos translation and the subsequent activations of mitogen-activated protein kinase and p90(rsk), probably by protein phosphorylation, seemed to have been inhibited by the microinjection of EGCG. These results suggest that EGCG may have the ability to control Xenopus oocyte maturation at least during the stage of Mos activation.

Animals↗
PubMed1999

Cloning and functional expression of an SGLT-1-like protein from the Xenopus laevis intestine.

A cDNA encoding an Na+-glucose cotransporter type 1 (SGLT-1)-like protein was cloned from the Xenopus laevis intestine by the 5'- and 3'-rapid amplification of cDNA ends method. The deduced amino acid sequence was 673 residues long, with a predicted mass of 74.1 kDa and 52-53% identity to mammalian SGLT-1s. This gene was expressed in the small intestine and kidney, reflecting a tissue distribution similar to that of SGLT-1. The function of the protein was studied using the two-microelectrode voltage-clamp technique after injection of cRNA into Xenopus laevis oocytes. Perfusion with myo-inositol elicited about twofold larger inward currents than perfusion with D-glucose. The order of the substrate specificity was myo-inositol > D-glucose > D-galactose >/= alpha-methyl-D-glucoside. The current induced by myo-inositol increased with membrane hyperpolarization and depended on external myo-inositol and Na+: the apparent Michaelis-Menten constant was 0.25 +/- 0.07 (SD) mM with myo-inositol, whereas the apparent concentration for half-maximal activation was 12.5 +/- 1.0 mM and the Hill coefficient was 1.6 +/- 0.1 with Na+. In conclusion, the cloned protein shares features with both SGLT-1 and the Na+-myo-inositol cotransporter.

Amino Acid Sequence↗
PubMed1997

Monocarboxylic acids enhance the anesthetic action of procaine by decreasing intracellular pH.

Sodium monocarboxylates are known to enhance the anesthetic action of procaine, and also decrease intracellular pH (pHi). We studied the effect of 30 mM Na monocarboxylates (formate, acetate, propionate, butyrate, and salicylate) on the pHi and on the anesthetic action of procaine HCl using giant axons of crayfish (Procambarus clarkii). The pHi was measured using pH sensitive microelectrode method and the anesthetic action was evaluated by the change in the action potential (AP) amplitude. The tested acids except for formate showed apparent decrease in pHi and enhancement of the action of 2 mM procaine. Other organic acids (maleate and benzensulfonate) did not affect pHi and anesthetic action of procaine. In the bicarbonate free solution, pHi increased and the anesthetic action was weakened. The EC25 values (the concentration of procaine which depresses the AP amplitude by 25%) of acetate, propionate, and bicarbonate free solution were coincided with the predicted EC25 values from the simple simulation on intracellular procaine increase according to the pHi change. But the EC25 value of salicylate group was less than half of the predicted. These results suggested that the enhancing action of straight chain monocarboxylic acids is due to pHi decrease, and salicylate has other additional mechanisms.

Action Potentials↗
PubMed1987

Enhancement of local anaesthesia action by organic acid salts. (I): Possible change of excitability in nerve fibre membrane.

Measurements of action potentials recorded from giant nerve fibres of the crayfish abdomen showed that by addition of an organic acid salt to a local anaesthetic solution, the onset of anaesthetic action became more rapid and the duration of action was prolonged. Similar results were also obtained in rat vagal nerves. The chemical structure of compounds having these enhancing effects was found to have, in common, a carboxyl group connected with either a benzene ring or an aliphatic hydrocarbon. Topical application of anaesthetic solutions to the skin of flexor side of the forearm in humans revealed that the duration of anaesthesia evaluated by the apparent decrease of the number of pain points was significantly prolonged by the addition of salicylate. The use of local anaesthetics combined with organic acid salts would be promising in clinical practice to enhance their action greatly.

Action Potentials↗
PubMed1979

Effects of nickel ion on the conduction of action potentials in non-myelinated nerve fibre of crayfish.

Effects of NiCL2 and PCMB (p-chloromercuribenzoate) on the action potential were examined by the method of extra- and intracellular electrodes, using a single nerve fibre of crayfish. The results obtained were as follows : The conduction of the action potential was blocked by treating the nerve fibre with Ni ion or PCMB. The blockade was easily recovered by replacement with cysteine. The process of the blockade and recovery, which could be repeated several times, was fairly characteristic such that the more repetition led the sooner blockade and the harder recovery. No conduction block was observed by treatment with Ni-cysteine mixed solution nor with PCMB-cysteine solution. The critical concentration for blocking was 1.1 x 10(-4)M for NiCL2 and 5.6 x 10(-6) M for PCMB. The action potential was disappeared without any change in the resting potential by treatment with the chemicals, which gave significant effects on the rising and falling phases of the action potential before the blockade.

Action Potentials↗
PubMed1978

Dynamic dialysis utilizing a hollow fibre unit as a rapid method for studying protein binding.

A useful approach for determining the binding ability which occurres in a protein-small molecule system was studied by a method of dynamic dialysis utilizing a hollow fibre unit. A dependency on experimental variables in the absence of protein was examined in order to characterize the nature of the dialytic process. It was shown that the rate of small molecule escaped across the dialytic membrane was dependent on stirring or flowing rate, temperature and pH, but independent on buffer strength or viscosity. The protein binding ability was examined by means of comparing amounts of small molecules in the absence and presence of protein. Kinetic parameters for the binding behaviour analysed from the Scatchard plot in the present experiment were in agreement with values previously reported.

Azo Compounds↗