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Biomedical subjects

Y Higuchi

Publications and source records attributed to Y Higuchi.

At least 379 records · Page 21Linked to original sources
PubMedDate not supplied

Squamous cell carcinoma of the oral cavity derived from a skin graft: a case report.

A case of oral cancer that had derived from a 19-year skin graft on the left buccal mucosa is reported. The patient had had three previous operations due to squamous cell carcinoma, erosion, and squamous cell carcinoma of the left buccal mucosa, respectively. In the last two operations, skin was transplanted, and the present cancer is believed to have derived from the latter one. The tumor was resected, and a new skin was grafted. In situ hybridization of human papilloma virus (HPV) was carried out; the HPV 16 DNA could not be detected in the specimen. Eight months later, a cervical lymph node metastasis was detected; thus, a radical neck dissection was performed.

Aged↗
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Multiple facial hemangiomas and iron-deficiency anemia: blue rubber-bleb nevus syndrome.

A 21-year-old female complained of bluish soft tumors on her face. Her past history of anemia led us to investigate a possibility of "Blue Rubber-Bleb Nevus syndrome." Subsequent examinations revealed a severe iron-deficiency anemia and gastrointestinal tract hemangiomas. The facial hemangiomas were removed after controlling anemia. It is important to examine the gastrointestinal tract when multiple cutaneous hemangiomas are associated with anemia.

Adult↗
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Increased neurons containing neuronal nitric oxide synthase in the brain of a hypoxic-ischemic neonatal rat model.

We evaluated the temporal profile of the number of neurons containing neuronal nitric oxide synthase (nNOS neurons) in the brain of a neonatal hypoxic-ischemic rat model. Hypoxic-ischemic insults were produced in the brains of 7-day-old rat pups using a combination of unilateral carotid artery ligation and hypoxic (8% oxygen) exposure. Sections of brain from rats killed at 0-24 h after the onset of hypoxia were stained immunohistochemically using a polyclonal anti-nNOS antibody. Histological changes of neuronal injury were evaluated in the adjacent Nissl stained sections. The number of nNOS neurons in the hemisphere ipsilateral to the carotid ligation was significantly increased (P < 0.05) at 3 h, when the neuronal injury consisted of clusters of degenerating hyperchromic neurons. Neuronal degeneration and an increased number of nNOS neurons were seen only in the ipsilateral hemisphere and the increase was most prominent in the dorsolateral area of the striatum. The increase in the number of nNOS neurons continued at 6 h, when the area of neuronal injury continued to expand. At 24 h, the neuronal injury was diffuse, and the number of nNOS neurons on the ipsilateral side significantly decreased. The increase of the number of nNOS neurons in the early phase of neonatal neuronal injury suggests its possible involvement in the hypoxic-ischemic injury. The delineation of its role in neuronal injury may lead to an improvement in managing neonatal hypoxic-ischemic brain injury.

Animals↗
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Close localization of mouse CD14 and CD32/16 in the cell surface of monocytic cell lines.

The binding of a rat anti-mouse CD14 monoclonal antibody (mAb) (rmC5-3) was inhibited by pretreatment of a mouse monocytic cell line WEHI-3 cells with anti-mouse CD32/16 mAb (2.4G2), whereas that of 2.4G2 was not inhibited by pretreatment of WEHI-3 cells with rmC5-3. An enzyme-linked immunosorbent assay showed that rmC5-3 detected peptide 9 corresponding to amino acid position 308-322 of CD14 but 2.4G2 did not. A Western blot analysis of sera revealed that rmC5-3 and 2.4G2 detected the bands thought to be soluble CD14 and CD32/16, respectively. rmC5-3 reacted with mouse CD14-transfected CHO cells, CD14-CHO-K1 cells, but 2.4G2 did not. Lipopolysaccharide-induced tumor necrosis factor (TNF)-alpha release was enhanced when a monocyte cell line (J774) was pretreated with rmC5-3. The enhancement was abolished by pretreatment with 2.4G2. The release of TNF-alpha was observed following treatment of J774 cells with 2.4G2 followed by anti-rat IgG F(ab')2.

Amino Acid Sequence↗
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Mouse soluble CD14 truncated at amino acid 71 in transgenic mice: preventive effect on endotoxin-mediated toxic shock.

Mouse soluble CD14 truncated at amino acid 71 (N71) contains the lipopolysaccharide (LPS)-binding sequence. Transgenic mice carrying alpha1-antitrypsin (AT) promoter-N71 fusion genes, designated AT363-1 and AT363-2, were produced. These mice constitutively produced elevated levels of N71. The concentration of LPS in sera after intraperitoneal LPS injection was lower in AT363-1 mice than in nontransgenic mice. The expression of N71 mRNA was enhanced by subcutaneous turpentine oil injection. The levels of serum LPS and tumor necrosis factor-alpha (TNF-alpha) after intraperitoneal LPS injections were lower in AT363-1 mice than in nontransgenic mice. Cell surface TNF-alpha and CD14 expression in exudate peritoneal macrophages prepared by intraperitoneal injection of proteose peptone and then LPS were higher in AT363-1 mice than in nontransgenic mice. Neutrophil infiltration in the liver after induction of the generalized Shwartzman reaction was lower in AT363-1 mice than in nontransgenic mice. Lethality of the Shwartzman reaction was significantly lower in AT363-1 than in nontransgenic mice. These findings suggest that the endotoxin-binding protein (N71) from CD14 prevents endotoxin-mediated toxic shock.

Animals↗
PubMedDate not supplied

Antitumor effects of soybean hypocotyls and soybeans on the mammary tumor induction by N-methyl-n-nitrosourea in F344 rats.

BACKGROUND: Soybeans are reported to have cancer inhibitory effects, probably due to their isoflavones. Soybean hypocotyls are embryo buds of soybeans and contain a higher amount of isoflavones and other factors than soybeans themselves. MATERIALS AND METHODS: The effects of soybean protein and soybean hypocotyls as diets on the development of N-methyl-n-nitrosourea (MNU) induced tumors were examined in female F344 rats. For this trial, 120 animals were used and at 6 weeks of age, groups of 30 animals were fed diets containing casein, soy protein isolate (SPI), 1.5% soybean hypocotyls and 5% soybean hypocotyls. Three weeks later all the animals except the control animals received a first dose (37.5 mg/kg body weight) of MNU by tail vein injection. At 29 weeks of age the animals received a second MNU dose (50 mg/kg body weight). Testing was performed 42 weeks after the first MNU dose. RESULTS: Analysis of cumulative palpable tumor incidence indicated that final tumor development of the SPI diet group and the hypocotyl diet groups was less than that of the casein diet group. Tumors were detected in one or more sites from 9 out of 24 rats in the casein diet group, 5 of 20 rats in SPI diet group, 6 out of 24 rats in the 1.5% hypocotyl diet group and 6 out of 23 rats in the 5% hypocotyl diet group. Pairwise comparisons indicated that the formation of tumors during the experiment was significantly less rapid in the SPI diet group and the hypocotyl diet groups than the casein group. No difference in tumor promotion was observed between the SPI diet group and the soybean hypocotyl diet groups. CONCLUSION: Our results suggest that dietary soybeans and soybean hypocotyls are capable of suppressing tumor promotion.

Animals↗
PubMedDate not supplied

19F-MRI of stomach and intestine using 50% FTPA emulsion under 2 T MRI system.

1H-MRI is of clinical value in many lesions, but imaging of gastrointestinal lesions is still difficult by 1H-MRI. To overcome this weak point of 1H-MRI, rabbit stomachs were examined by 19F-MRI using 50% FTPA emulsion. We also examined the stability of 50% FTPA emulsion in the stomach and its absorption from the gastrointestinal tract. We found that 50% FTPA emulsion was very stable at pH 1.5, and only a very small amount was absorbed. A rabbit (weighing 2 kg) was anesthetized, and 100 ml of 50% FTPA emulsion was infused into the stomach by catheter. 19F-MRI was performed in this rabbit using a 2 T superconducting MRI system designed for human use, and clear pictures of the stomach were obtained. From our results we conclude that 19F-MRI of the stomach using 50% FTPA emulsion is of practical value.

Animals↗
PubMedDate not supplied

Cytotoxic and antitumor effect of a fraction from cell-free extract of group A Streptococcus on transplantable tumor cells.

A fraction (60F) which had cytotoxic and antitumor activity could be obtained by precipitating cell-free extract (CFE) of group A streptococcus (Su strain) with a 50% to 60% saturation of ammonium sulfate. 60F exhibited a potent inhibitory effect on the incorporation of 3H-thymidine into various types of transplantable tumor cells. The activity of 60F was reduced by proteases and heating at 45 degrees C, but not by glycosidases and nucleases. Furthermore, 60F showed antitumor activity, such as cure and prolongation of life, in animals bearing EAC, MM-2, and S-180 tumors. These results show that 60F is probably protein and essentially exerts cytotoxic action against every cell line of tumor tested in vitro, and that the antitumor activity of 60F in vivo depends on the transplantability of tumor cells.

Animals↗
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Solubilization and purification of bone morphogenetic protein (BMP) from Dunn osteosarcoma.

The 4 M GuHCl soluble proteins of Dunn osteosarcoma were fractionated in two steps by means of CsCl density gradient centrifugation. Further purification of bone morphogenetic protein (BMP) was accomplished by molecular sieve techniques utilizing thin-channel diafiltration (Amicon), Sepharose CL-6B and Sephacryl S-200 gel filtration. Partially purified BMP fractions were analyzed by electrophoresis on 7.5% SDS polyacrylamide gel. The molecular weight of BMP active fractions ranges from 30,000 to a few thousand daltons. BMP may be one of the two proteins of MW of 12,500 and 16,000 daltons.

Animals↗