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Biomedical subjects

Y Higuchi

Publications and source records attributed to Y Higuchi.

At least 217 records · Page 12Linked to original sources
PubMed1989

Dimeric substance P analogue shows a highly potent activity of the in vivo salivary secretion in the rat.

We have synthesized a dimeric analogue of substance P (SP) COOH-terminal nonapeptide fragment (D-SP-(3-11] and examined the in vivo and in vitro biological activities in the submaxillary gland of the rat. The dimer elicited an enhanced biological response as compared with receptor binding, showing 2.4-fold more potent receptor affinity than its monomer and 75-fold more potent in vivo salivary secretion activity.

Animals↗
PubMed1989

The laser treatment of hemorrhoids: results of a study on 1816 patients.

Laser is an effective, simple and harmless clinical procedure used for the treatment of hemorrhoids, as an alternative to medical therapy or surgery. In this report, we describe our experience of applying carbon dioxide laser to hemorrhoids in a total 1816 consecutive patients. The results lead us to conclude that the laser treatment of hemorrhoids is effective in pain alleviation from the first session and that patients so treated have a much more comfortable postoperative course.

Female↗
PubMed1989

Insertional activation of N-myc by endogenous Moloney-like murine retrovirus sequences in macrophage cell lines derived from myeloma cell line-macrophage hybrids.

Hybrids formed from a myeloma cell line, NS1, and macrophages initially show myeloma properties but later, after loss of the parental macrophage genome and consequent loss of myeloma characteristics, express macrophage properties. Molecular studies demonstrated that macrophage properties in the hybridomas originate from the NS1 parental cells (M. Setoguchi, S. Yoshida, Y. Higuchi, S. Akizuki, and S. Yamamoto, Somatic Cell Mol. Genet. 14:427-438, 1988). In such hybrids, N-myc was activated by insertion of endogenous Moloney-like retrovirus sequences into mouse N-myc exon 3 when the hybrids gained macrophage properties. Interestingly, expression of N-myc took place in all aged hybrids. These results suggest that such unique insertional mutagenesis occurs in a regionally specific manner and that expression of N-myc may play a role in hematopoietic lineage conversion.

Amino Acid Sequence↗
PubMed1989

Pattern of ovarian steroid secretion during ovulation of in vitro perfused rat ovaries varies with method of sampling.

Wistar rats were primed at 25 days of age with 20 i.u. PMSG s.c., followed 48 h later with 100 ng LH/ml to induce the ovulatory process in vitro. Assays for progesterone, oestradiol, and testosterone were performed on samples taken at fixed intervals of 0, 1, 2, 4, 8 and 12 h after LH administration. The results show that, while there was some degree of correlation in the concentrations of progesterone between the ovarian extract and perfusate, there were very large differences in the measurable amounts of testosterone and oestrogen in the fluid samples and ovarian homogenates. Therefore, taking samples from the perfusate may not provide a good assessment of the steroidogenic activity in the ovary.

Animals↗
PubMed1989

[Objective examination and diagnosis of bruxism. 1. New device for recording muscle activity and tooth contacts during sleeping at home].

The purpose of this study was to make an objective diagnosis of bruxism using a new recording system by observation during sleeping at home. The system was composed of a portable recorder for monitoring bruxism at home and an analyzing device for recalling the data later in laboratory. Muscle activity as recorded by EMG, occlusal tooth contacts were monitored by microvibration pick-up, and grinding sounds were recorded by a small microphone, simultaneously. These were printed on the one recording paper by playing back the tape. Six subjects were selected and were monitored individually for five nights with this new system at home. Records were transcribed and analyzed in the laboratory. As a result, nocturnal muscle activity, occlusal tooth contacts, and grinding sounds were recorded and analyzed efficiently with system. This new system is considered in useful for the investigation and diagnosis of bruxism in periodontal disease.

Bruxism↗
PubMed1989

[Prolapse of the buccal fat pad in infant].

Two infant cases with traumatic prolapse of the buccal fat pad were presented. They suffered injuries from objects held in the mouth, such as a spoon and a tooth brush. Both received surgical treatment under local anesthesia, including closure of the wound with sutures. As the prolapsed tissue was fresh in the first case, it was repositioned. However, since the second case had passed 3 days after the accident and had a sign of infection, the prolapsed tissue was totally removed. Postoperative course was uneventful and there was no recurrence in both cases.

Adipose Tissue↗
PubMed1988

Purification and characterization of a novel cytotoxic substance from cell-free extract of Streptococcus pyogenes.

A cytotoxic substance designated as streptococcal cytotoxic protein (SCP) was isolated from a cell-free extract of the Su strain of Streptococcus pyogenes possessing cytotoxic and antitumor activity. SCP was purified with a series of column chromatography and preparative PAGE to give a homogeneous single band as revealed by PAGE analysis. The purified SCP has a molecular mass of 165 kDa, composed of four 43 kDa subunits, and its pI is 4.3. SCP was sensitive to proteinases and was labile to heat and at acidic or alkaline pH. SCP showed inhibitory effects on the [3H]thymidine, [3H]uridine and [3H]leucine uptakes and on the growth of cells, and released 51Cr from cells when the protein was added to the cultures of Ehrlich ascites carcinoma (EAC), mouse mammary tumor (MM-2), leukemia (L-1210) and NIH-3T3 mammalian cells in vitro. SCP also showed an antitumor effect on EAC or MM-2 tumor-bearing mice but not on L-1210 tumor-bearing mice in vivo.

Amino Acids↗
PubMed1988

Late occurring recurrence of oral cancer after combined treatment with bleomycin and radiotherapy.

Among 11 patients with oral cancer that disappeared after combined treatment with bleomycin and radiotherapy without surgery, one had an early recurrence and three had recurrences after 2 years or more. These late occurring recurrences were microcancers on mucous membranes that were thought to have been cured. Two of the three patients whose tumors recurred and another patient were found to have epithelial dysplasia at 1 year and 2 months, 4 years and 2 months, and 1 year and 3 months later, respectively. Among 59 patients who underwent surgery after the combined treatment, no viable cancer cells were found in the resected specimens of 20 patients, but moderate or severe epithelial dysplasia was noted in ten of the 20 patients. The present findings imply that patients in whom an apparent cure has been brought about by conservative treatment may harbor latent malignancy.

Adult↗
PubMed1988

Molecular analysis of expression of parental cell properties in hybrids between monocytes and a myeloma cell line.

Interspecific hybrid cell lines (HINS lines) between human monocytes and a mouse (H-2d) myeloma cell line, NS1, initially showed myeloma properties, but later expressed macrophage properties, while losing myeloma cell characteristics and undergoing a marked reduction in the number of chromosomes. The aged HINS cells showed mouse but not human phenotypes. Molecular genetic analysis demonstrated loss of BLUR8 genes, expression of mouse macrophage specific genes, and enhanced transcription of H-2d and mouse beta 2-microglobulin genes in the aged cells, confirming that the hybrids' macrophage properties were attributable to NS1 genes. Transcription of kappa light chain genes was not detectable in aged HINS cells, although the genes were retained. Since some rearrangements of the genes were suggested and cycloheximide treatment induced no transcription of the genes, cis mechanisms might be involved in the loss of expression of the genes.

Animals↗
PubMed1988

An autopsy case of human T-lymphotropic virus type I-associated myelopathy.

This report describes the first autopsy case of human T-lymphotropic virus type I (HTLV-I)-associated myelopathy (HAM). The disease mainly affected the spinal cord, particularly the lateral and anterior columns, where loss of myelin and axon was observed. The changes were bilateral and occurred mainly along the tract. Perivascular and parenchymal infiltration with lymphocytes and macrophages, as well as astrocytosis, were observed in the white and grey matters of the spinal cord. Blood vessels in the spinal cord and in the subarachnoid space of the spinal cord showed hyalinoid thickening of media and adventitia associated with infiltration of lymphocytes. These findings are similar to those of tropical spastic paraparesis (TSP).

Autopsy↗
PubMed1988

Mandibular ameloblastoma associated with salivary gland tumor.

2 cases of mandibular ameloblastoma associated with salivary gland tumor are presented. Case no. 1, a 32-year-old male was diagnosed as mandibular ameloblastoma and acinic cell tumor of the submandibular gland, both of which were found on the left side. Case no. 2, a 62-year-old male was diagnosed as recurrent ameloblastoma of the mandible and adenolymphoma adjacent to a lower pole of the parotid gland.

Adenolymphoma↗
PubMed1988

Crystallization and preliminary X-ray studies on human epidermal growth factor.

Single crystals of human epidermal growth factor have been prepared from a polyethylene glycol solution and characterized by X-ray diffraction. The crystals grow in a space group of P2(1) with unit cell dimensions of a = 32.7, b = 32.5, c = 22.3 A, and beta = 96.9 degrees. They contain a single molecule per asymmetric unit and show better diffraction than 2.5 A.

Crystallization↗