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Biomedical subjects

Y Handa

Publications and source records attributed to Y Handa.

At least 181 records · Page 10Linked to original sources
PubMed1986

Perfusion technique of suckling rat liver, and comparison of cytologic and biochemical properties between hepatocytes isolated from suckling and adult rats.

Collagenase-liver-perfusion technique, currently used with adult rat liver, was applied to isolation of hepatocytes from suckling rat liver. The total hepatocyte numbers isolated from suckling rats by collagenase-liver-perfusion technique were 9-fold higher than those by non-perfusion technique. The yield and viability of isolated hepatocytes from suckling rats were 18.1 X 10(7) cells per gram liver and 95%, respectively. The cell yield per gram liver and viability from suckling rats were 185% and 112% of those from adult ones, respectively. In comparison with adult rat hepatocytes, suckling rat hepatocytes were smaller and more homogeneous. The percentage (3.1%) of binucleate cells in the hepatocytes isolated from suckling rats was about one tenth of that (30.7%) in the hepatocytes isolated from adult rats. The isolated suckling rat hepatocytes had higher tyrosine aminotransferase (TAT) and glucose-6-phosphate (G6Pase) activities than adult ones. Suckling and adult rat hepatocytes were transferred to primary culture to compare their cell number kinetics and functional longevities. The functional longevities of those hepatocytes were assessed by their capacity to secrete albumin and alpha-fetoprotein (AFP) into the culture medium and to express TAT and G6Pase activities up to day 6 of primary culture.

Albumins↗
PubMed1986

Treatment of blow-out fracture.

Fifteen patients with blow-out fractures were treated in this series. Two patients were treated conservatively and the thirteen others were treated surgically with the transorbital approach. An infraorbital or medial orbital skin incision was made. The periosteum was dissected and entrapment of orbital fat tissue and muscle were resolved. The floor and/or medial wall were reinforced with tantalum mesh (Codman). The operative results were good in 12 patients and fair in one patient, who retained some diplopia. The tantalum mesh is easy to handle and strong enough to reinforce the orbital wall. It can be seen on plain skull film and makes little artifact on CT scan. This mesh is good material for repairing blow-out fractures.

Adolescent↗
PubMed1986

Thyrotropin-stimulated electrical excitation and its refractoriness in cultured porcine thyroid cells.

This report demonstrates TSH-stimulated electrical excitation in the thyroid and its refractoriness after exposure to TSH. TSH depolarizes the membrane potentials and causes action potentials. TSH-induced electrical activity is characterized by a latent period, rapid depolarization, action potentials (usually two spikes were observed), and then repolarization to the potential level of the silent phase. This TSH-induced electrical excitation is associated with iodide discharge. Previous exposure to TSH induces refractoriness of electrophysiological excitation and iodide discharge to further TSH stimulation.

Animals↗
PubMed1986

Functional recovery of locomotive behavior in the adult spinal dog.

Complete transection of the midthoracic spinal cord was performed on adult female dogs. Development of reflexes and locomotive movements in the hindlimbs after spinal transection was investigated over several months. In the earlier stages after the surgery, dogs showed flaccid paralysis of the hindlimbs. Within several weeks, muscle tone of the hindlimbs was gradually increased accompanied by development of flexion reflex with after-discharge in addition to monosynaptic reflexes. Alternating stepping movement also began to develop. Afterward, extensor thrust and crossed extension reflex were observed. Standing behavior of the hindlimbs was found after enough development of the extensor thrust and correct placement of the pads of the toes. Steady development of stepping and standing caused forward locomotion using fore- and hindlimbs. This walking behavior of the hindlimbs was never inhibited by additional spinal cord transection. These findings indicate that the isolated spinal cord caudal to the transected site is responsible for the locomotive behavior of the hindlimbs in adult spinal dogs. Spinal automatism and development of responses induced by afferent inflow from outside the cord may help to explain such functional recovery of the paralyzed hindlimbs.

Animals↗
PubMed1986

Comparative study of proliferation of suckling and adult rat hepatocytes in primary culture in response to growth-stimulating factors.

The replicative responses of suckling and adult rat hepatocytes in primary culture to growth-stimulating factors were compared. By addition of L-proline alone, the [3H]-thymidine labeling of suckling rat hepatocytes was dramatically enhanced, but that of adult ones was not. Epidermal growth factor (EGF), insulin, triiodothyronine (T3) and glucagon also enhanced the labeling of suckling rat hepatocytes regardless of the presence or the absence of L-proline. On the other hand, in the absence of L-proline, only EGF enhanced the labeling of adult rat hepatocytes, and, in the presence of L-proline, insulin as well as EGF enhanced the labeling. In the presence of growth factors and L-proline, the number of suckling rat hepatocytes increased up to about 143%, whereas that of adult rat hepatocytes hardly increased. Thus, a remarkable difference in replicative responses to growth factors and L-proline was observed between suckling and adult rat hepatocytes in primary culture.

Aging↗
PubMed1986

A case of multiple endocrine adenomatosis type 1.

A case of pituitary adenoma associated with pancreatic islet cell tumor is presented. A 29-year-old man with symptoms of confusion and abnormal behaviour was admitted to the neurosurgical department. He was diagnosed as suffering from multiple endocrine adenomatosis (MEA) type 1. Screening of his family members revealed that his mother had high levels of gastrin, glucagon and parathormone and his father had a high level of gastrin. The pituitary adenoma and pancreatic tumor were removed satisfactorily. Family members as well as the patient should be checked periodically.

Adenoma, Islet Cell↗
PubMed1986

[Cerebral blood flow studies using N-isopropyl I-123 p-iodoamphetamine in cerebral ischemic lesions].

Eighteen patients were studied for cerebral blood perfusion abnormalities using N-isopropyl I-123 p-iodoamphetamine (IMP) and rotating dual gamma camera emission computed tomography (ECT). All were stroke patients, 10 with cerebral vasospasm after an aneurysmal rupture, 3 with an occlusion of the middle cerebral artery, 2 with an occlusion of the internal carotid artery (IC), one with an IC stenosis, one with Moyamoya disease and one with RIND. Four patients had extracranial-intracranial (EC-IC) bypass operations. In three of them, CBF studies were done before and after bypass surgery. An arterial line was placed in the left radial artery and connected to a Harvard pump. IMP (1.5-3 mCi) was injected into an arm vein while at the same time an arterial blood sample was withdrawn at a constant speed for 5 minutes. Scanning was started 35 minutes after IMP injection. After a scan, multiple transverse, coronal and sagittal section images were reconstructed with a minicomputer. We determined the values of regional CBF in the regions of interest using an image. Transmission computed tomography (CT) studies were performed on the same day. In eight patients, CBF study by 133Xe inhalation method (NOVO cerebrograph) was done. ECT showed diffuse low perfusion in two patients and focal low perfusion in 16 patients while CT showed abnormalities in 9 patients (50%). ECT abnormalities were more extensive than CT abnormalities. The values of rCBF in the superficial brain determined by ECT were similar to those examined by the inhalation method (ISI). Significant increase in rCBF was observed after the bypass operations.(ABSTRACT TRUNCATED AT 250 WORDS)

Adult↗
PubMed1986

[Pharmacokinetics and toxicity of intrathecal administration of recombinant interleukin 2].

In order to contribute to the development of adoptive immunotherapy against malignant brain tumors, the pharmacokinetics and toxicity of intrathecally administered recombinant interleukin-2 in dogs and human patients were analyzed. The pharmacokinetics showed that a high concentration of IL-2 was maintained in the intrathecal cavity in both dogs and human (t1/2 = 1.41 and 1.68 hours, respectively) after administration. However, no activity of IL-2 was detected in the cerebrospinal fluid after the systemic administration of rIL-2 in one dog. No meningitis, ventriculitis or degeneration of neurons was seen histopathologically in dogs 3 weeks after the intrathecal administration of rIL-2 (200 units). A high concentration of IL-2 in the tumor cavity was maintained for a very long time (t1/2 = 14.8 hours) after the intratumoral administration of rIL-2 in one of the patients. Although low-grade fever and mild headache were sometimes observed after the intrathecal administration of rIL-2 in patients, there was no other side effect mentioned. Intrathecal or intratumoral administration of rIL-2 appeared to be an valuable procedure which should be evaluated in conjunction with adoptive immunotherapy against malignant brain tumors.

Adult↗
PubMed1986

[Brain blood flow studies with single photon emission computed tomography in patients with plateau waves].

The authors studied brain blood flow with single photon emission computed tomography (SPECT) in two patients with plateau waves. The intracranial pressure and blood pressure were also monitored continuously in these patients. They included one patient with brain-tumor (rt. sphenoid ridge meningioma) and another with hydrocephalus after subarachnoid hemorrhage due to rupture of lt. internal carotid aneurysm. The intracranial pressure was monitored through an indwelling ventricular catheter attached to a pressure transducer. The blood pressure was recorded through an intraarterial catheter placed in the dorsalis pedis artery. Brain blood flow was studied with Headtome SET-011 (manufactured by Shimazu Co., Ltd.). For this flow measurement study, an intravenous injection of Xenon-133 of about 30 mCi was given via an antecubital vein. The position of the slice for the SPECT was selected so as to obtain information not only from the cerebral hemisphere but also from the brain stem: a cross section 25 degrees over the orbito-meatal line, passing through the inferior aspect of the frontal horn, the basal ganglia, the lower recessus of the third ventricle and the brain stem. The results indicated that, in the cerebral hemisphere, plateau waves were accompanied by a decrease in blood flow, whereas, in the brain stem, the blood flow showed little change during plateau waves as compared with the interval phase between two plateau waves. These observations may explain why there is no rise in the blood pressure and why patients are often alert during plateau waves.

Adult↗
PubMed1986

[Effects of the subarachnoid hemorrhage on the release of arachidonate metabolites from canine cerebral arteries].

Release of arachidonate metabolites from isolated canine cerebral arteries into perfusing medium were estimated using radioimmunoassay (RIA) in vitro. The cerebral arteries were isolated from dogs sustained experimental subarachnoid hemorrhages (SAH) and the results were compared with that of normal canine cerebral arteries. The amount of 6-Keto-PG F1 alpha (stable metabolite of PGI2) and PGE2 released from normal cerebral arteries were 455 +/- 84 (n = 7) and 177 +/- 72 (n = 8) ng/min/g dry weight (mean +/- SEM), respectively. Among other arachidonate metabolites, TXB2 (stable metabolite of TXA2), PGF2 alpha, PGD2 were also measured, but release of these arachidonate metabolites were little compared with PGI2 or PGE2. The amount of 6-Keto-PGF1 alpha and PGE2 released from the cerebral arteries subjected to subarachnoid hemorrhage were 110 +/- 34 (n = 6), 169 +/- 40 (n = 6) ng/min/g dry weight respectively. In SAH group, release of 6-Keto-PGF1 alpha had diminished remarkably, but no remarkable quantitative change were seen among other arachidonate metabolite between normal and SAH groups. The diminution of PGI2 release in the cerebral artery subjected to SAH may be involved in the pathogenesis of cerebral vasospasm. The release of PGs from canine pial arteries induced by the exposure of the pial arteries to red blood cell hemolysate was also estimated by RIA. The release of PGE2 tended to increase following to exposure to hemolysate but no other arachidonate was increased.

Animals↗
PubMed1986

Isotope cisternography in patients with intracranial hypertension.

Cerebrospinal fluid flow (CSF) was studied using isotope cisternography in 52 patients with increased intracranial pressure (ICP), all of whom showed acute transient rises of ICP, i.e., plateau waves, in their continuous ICP recordings. The patients were assigned to two groups. Group I was comprised of 23 patients without hydrocephalus and high ICP resulting from brain tumors, benign intracranial hypertension, and superior sagittal sinus thrombosis. Group II included 29 patients with either communicating hydrocephalus or high ICP resulting from rupture of intracranial aneurysm. Plateau waves were frequently observed in patients with baseline pressures ranging from 21 to 40 mmHg in both groups. The isotope cisternographic pattern in the Group I patients showed a large accumulation of radioactivity over the cerebral convexities, while that in the Group II patients revealed a complete obstruction of the subarachnoid space over both cerebral convexities. The isotope clearance from the intracranial CSF showed a marked delay in both groups of patients with one exception. The results suggest that, in the limited range of increased ICP caused by delayed CSF absorption, plateau waves are most evident regardless of the isotope cisternographic pattern.

Adult↗
PubMed1986

[Cockayne's syndrome presenting cerebral ischemic attack: case report].

A 29-year-old man with Cockayne's syndrome (CS), presenting reversible ischemic neurological deficit is reported. In his past history, hearing disturbance developed at 6 years old and visual disturbance at 12 years old. His parents have consanguinious marriage. He came to our hospital complaining of right-sided hemiparesis and speech disturbance. He was 115.8 cm tall and his weight 20 kg. The characteristic manifestation of CS, i. e., dwarfism, mental retardadation, cachectic feature, retinal atrophy, neural deafness and calcification of bilateral basal ganglia were all noticed. A CT scan on admission revealed marked brain atrophy as well as the intracranial calcifications, while no lesions compatible with his neurological findings were detected. Cerebral ischemic state was mostly suspected. Following up with conservative therapy by the use of fibrinolytic agent, his neurological deteriorations disappeared on the 4th hospital day. Cerebral angiograms showed stenotic lesions of both C1-C2 portion of the left internal carotid artery and the right middle cerebral artery, and the aneurysm in the right internal carotid artery. Such atherosclerotic vascular change as observed in the cerebral angiograms in this case have progressed rapidly for his age. In this case, diabetes mellitus and hyperlipoproteinemia such as increased total cholesterol, increased triglyceride, decreased HDL and increased apoprotein B and C II were complicated for the risk factor of the atherosclerosis. It's controversial that early progress of atherosclerosis is due to ideopathic original feature of CS or to the secondary change from these complications.(ABSTRACT TRUNCATED AT 250 WORDS)

Adult↗
PubMed1985

TSH-stimulated electrical excitation in thyroid cells.

This report demonstrates TSH-stimulated electrical excitation in cultured porcine thyroid cells. TSH depolarizes the thyroid cell membrane potentials and causes the appearance of action potentials, which occur in a burst. The burst is preceded by depolarization and after the burst, during which usually 2 spikes are seen, a repolarization occurs. This TSH-induced electrical excitation is associated with iodide discharge.

Animals↗
PubMed1985

Effect of phenobarbital on adult rat liver cells treated with 3'-methyl-4-dimethylaminoazobenzene in primary culture.

The effect of phenobarbital (PB) on liver cells treated with 3'-methyl-4-dimethylaminoazobenzene (3'-Me-DAB) was studied using primary cultures of normal adult rat liver cells. Following a 1-day attachment period, primary liver cell cultures were treated with 0.24 mM 3'-Me-DAB for 6 days, and then treated with or without PB at 0.75, 1.5 and 3 mM for 19 days. Similarly, control cultures were treated with 0.5% dimethylsulfoxide (DMSO), a solvent for 3'-Me-DAB, for 6 days, and then treated with or without PB in the same way. Each treatment was done on 8 cultures. Chromosome analysis and cytochemical assay for gamma-glutamyltranspeptidase (GGT) activity were carried out on the carcinogen-treated and control cultures between 1 and 2 months after initiation of primary culture. Chromosomal abnormalities were detected in 23 of 32 carcinogen-treated cultures and also in 2 of 28 control cultures tested. However, GGT positive cells were detected only in the carcinogen-treated cultures at a frequency of 22/32. Of the 23 carcinogen-treated cultures with chromosomal abnormalities, 18 contained GGT positive cells. These results show a good correlation between chromosomal abnormality and acquisition of GGT activity at culture dish level. Furthermore, in the carcinogen-treated cultures, PB treatment caused a dose-dependent increase in the number of GGT positive cultures and in the percentage of GGT positive cells in each culture, and also caused a dose-dependent increase in the number of cultures with chromosomal abnormalities.

Animals↗
PubMed1985

Giant cell tumor of the skull.

Two cases of giant cell tumor of the skull are reported. The first patient had a radical operation for a giant cell tumor of the temporal base followed by radiation therapy. The second patient had a partial removal of a giant cell tumor of the occipital bone followed by radiation therapy. The pertinent literature on giant cell tumors originating from the cranial bones other than the sphenoid bone is reviewed.

Adolescent↗
PubMed1985

Hemolysate-induced release of prostaglandinlike substances from the canine cerebral arteries.

The release of prostaglandinlike substances from canine pial arteries that was induced by exposure of the pial arteries to red blood cell hemolysate was estimated by using a superfusion technique for prostaglandin bioassay. The assay organs used were strips of rat stomach for prostaglandin E2- or prostaglandin F2 alpha-like substances, strips of dog coronary artery for prostaglandin I2-like substance, and strips of dog ileum for prostaglandin F2 alpha-like substance. The substances released from the canine pial arteries induced contractions in rat stomach strips and relaxations in canine coronary arterial strips, whereas they did not induce any response in canine ileal strips. The equivalent prostaglandin E2 doses for the contractions of the rat stomach strips and the equivalent prostaglandin I2 doses for the relaxations of the canine coronary arterial strips were 125.2 +/- 19.4 (n = 8) and 59.5 +/- 16.4 (n = 6) pmol/g wet wt +/- SEM, respectively.

Animals↗