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Biomedical subjects

Y H Zhou

Publications and source records attributed to Y H Zhou.

9 recordsLinked to original sources
PubMed1989

Isolation and cultivation of protoplasts of Caloglossa leprieurii.

Protoplasts, as important media in genetic engineering, had been isolated from some genera of seaweeds in the beginning of this decade. Using a mixed enzyme solution composed of 3% Sea snail enzyme, 3% Cellulase Onozuka R-10, 1% Macerozyme and 0.5% Pectinase, viable protoplasts were isolated from Caloglossa leprieurii (Mont) J. Ag. (Ceramiales, Rhodophyta). The viability of protoplast was determined by its exclusion of Evans Blue stain. The absence of cell walls were observed in the protoplasts, and were determined by Calcofluor White, reduced osmolarity, electron microscopic examination and self-fusion. About half of the protoplasts survived and divided at a high rate when they were cultivated in a thin liquid layer of modified medium No.5 (MES as basic medium, supplemented with MS medium, vitamins and 1ppm IAA, 1.5 ppm KT). Regenerated plantlet appeared after 45 days. Tetrasporangia appeared in the blade of some regenerated plants after 4 months. Due to its properties of Being large, polynucleal and regenerative, protoplasts of C. leprieurii offer a good prospect in the field of cell fusion and genetic operation of seaweeds.

Cell Division↗
PubMed1989

[Observation on the development of Plasmodium falciparum in Anopheles dirus].

This paper is a record of our observation on the stages of development of Plasmodium falciparum in Anopheles dirus. The malarial parasites were derived from 5 infected patients living in Guizhou Province and used to infect 8 batches of An.dirus. The morphology of various developmental stages studied under light microscope and their average size were as follows: Microgametes were filament-shaped, 13.31 +/- 2.22 microns; macrogametes and zygotes oval or round, 4.36 +/- 0.59 microns and 3.39 +/- 0.39 microns respectively; ookinetes banana shaped, 13.56 +/- 0.80 microns x 2.90 +/- 0.48 microns; oocysts ovoid or spherical in shape, the smallest one being 7.086 microns in equivalate diameter (2 days old) and the largest one 72.60 microns (11 day old); slender sporozoites measured 10.625 +/- 0.82 microns x 1.179 +/- 1.3 microns. The late sporogonic stage of P. falciparum was observed with scanning electron microscope. The sporozoite buds developed on the surface of the sporoblast bodies, being round or elliptic or irregular-shaped. The anterior end of sporozoites was truncate and sometimes a micropyle could be seen at a distance 1/3 from the anterior end. A description was given of the different characteristics of the macrogametes and zygotes, together with the arrangement of the pigment granules of oocysts.

Animals↗