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Biomedical subjects

Y Furuya

Publications and source records attributed to Y Furuya.

At least 235 records · Page 13Linked to original sources
PubMed1984

Production and characterization of monoclonal antibodies specific to sweet clover necrotic mosaic virus.

Monoclonal antibodies were produced in vitro by fusing mouse myeloma cells P3-X63-AgU1 with spleen cells derived from BALB/c mice immunized with purified sweet clover necrotic mosaic virus (SCNMV). Twenty-one out of 47 clones which secreted monoclonal antibodies of high titres against SCNMV were injected intraperitoneally into mice previously primed with Pristane. The ascites fluid harvested 10 to 14 days later showed a strong and specific anti-SCNMV activity in reverse passive haemagglutination inhibition, passive haemagglutination and indirect enzyme linked immunosorbent assay. The monoclonal antibodies of the 21 clones did not react with red clover necrotic mosaic virus (Swedish isolate). The class and subclass of immunoglobulins of the monoclonal antibodies secreted by established cultures were determined to be IgG2a for 15 clones and IgG3 for 6 clones.

Animals↗
PubMed1984

Computerized tomography of cranial sutures. Part 1: Comparison of suture anatomy in children and adults.

Knowledge of normal suture anatomy and development is vital in order to understand abnormal suture development and to be able to distinguish sutures radiographically from normal anatomical structures and possible skull fractures. The anatomy of the sutures and synchondroses of 150 normal pediatric and adult patients was studied using high-resolution computerized tomography scanning. Sutures of both the calvaria and skull base were most accurately identified in axial and coronal high-resolution thin-section scans when bone window algorithms were used. Developmental changes of the sutures and synchodroses , the inner and outer tables, and the diploic space were all well delineated. Vault sutures could be identified routinely in children, but their presence in adults varied considerably. With increasing age, parasutural sclerosis developed and sutures were more closely apposed.

Adolescent↗
PubMed1984

Computerized tomography of cranial sutures. Part 2: Abnormalities of sutures and skull deformity in craniosynostosis.

Preoperative computerized tomographic (CT) scans of 24 children who had surgery for either single or multiple craniosynostoses were compared with skull radiographs and operative and pathological findings. In addition to providing accurate imaging of calvarial and skull base deformities secondary to premature suture closure, high-resolution CT with bone definition algorithms supplied valuable detail of anatomical changes at the abnormally developed suture. The CT findings varied with the location of the suture. Thickened bony ridges predominated at the sagittal suture, focal bone thickening and erosions were more likely to be found at the metopic suture, and parasutural sclerosis was the prevalent finding on one side of the lambdoid suture. No evidence of the suture could be detected in the majority of patients with complete coronal craniosynostosis. Radiographs of the skull were shown to be a relatively insensitive means of imaging the zone of limited fusion, especially the lambdoid suture. An excellent correlation was found between the CT scan and the operative and pathological findings. There was histological evidence of progressive suture fusion in virtually all patients. An asymmetrically narrowed lucent zone with parasutural sclerosis or bony ridges seen on CT scans correlated well with fibrous union of the suture found on histological examination. The authors conclude that high-definition CT used in conjunction with bone windows and thin and coronal slices for the evaluation of sagittal sutures is a useful imaging method for the evaluation of craniosynostosis.

Cranial Sutures↗
PubMed1984

Use of monoclonal antibodies in the assay of hepatitis B core antigen and antibody.

Hybridoma cells secreting antibody against hepatitis B core antigen (HBc Ag) were prepared. BALB/c mice were immunized with 0.2 ml of purified HBc Ag, and their spleen cells were fused with mouse myeloma (P3U1) cells by means of polyethylene glycol 1000. Activities of antibodies against HBc Ag (anti-HBc) were tested by the immune adherence hemagglutination (IAHA) and reverse passive hemagglutination inhibition (RPHI) techniques. Hybridoma cells found to contain antibodies accounted for 26.5% by IAHA and 52.1% by RPHI, respectively. Among 32 monoclonal anti-HBc antibodies, 18 were found to be positive by both IAHA and RPHI, and the remaining 14 positive by RPHI only. After cloning, they were injected intraperitoneally into ascitic mice. The highest anti-HBc activity with an IAHA titer of 1:4 X 10(6) and with an RPHI titer of 1:1 X 10(5) was detected in this ascitic fluid. Enzyme immunoassay (EIA) and RPHI with monoclonal antibody containing the highest anti-HBc activity were developed. All the sera in which anti-HBc was detected by IAHA and RPHI with polyclonal antibody were positive in EIA. RPHI titers obtained with monoclonal antibody were in good agreement with usual IAHA and RPHI titers obtained with polyclonal antibody. These results indicate that monoclonal antibody can be used in the HBc Ag and anti-HBc assay system.

Animals↗
PubMed1983

[Effect of heating on COHb% of the blood].

The blood from burned cadavers heat-coagulates badly and COHb% at autopsy is expected to be lower than that at death. To study such an effect of heating on COHb% in the blood, we have carried out experiments on heating of dead bodies of CO intoxicated guinea pigs as well as that of blood containing COHb in vitro. Three high temperatures (300 degrees C, 500 degrees C, 700 degrees C) and five exposure times (5 min, 10 min, 15 min, 20 min, 30 min) were used to stimulate conditions of real fire. Supposing that the effect of heat can be expressed in terms of a product of heating temperature with exposure time, a relation between the product and CO liberation rate was examined. The release of CO is at most 20% of the CO initially present when the product is under 5,000 and the blood retains still fluid, whereas the release of CO is about 50% when the product is over 10,000 and the blood is clotted. It is difficult to adapt CO release from COHb observed in experiments on heating in vitro and in the dead body to the judgment of the causes of death of burnt corpses, but if taking into consideration the degree of burns of charred bodies and the degree of the heat coagulation of the blood, it is suggested that one is able to anticipate COHb% at death from the gas chromatographic measurement of COHb% at autopsy in medico-legal practice.

Animals↗