Biomedical subjects

Y Eishi

Publications and source records attributed to Y Eishi.

58 records · Page 4Linked to original sources

PubMed1981

Demonstration of various antigens on paraffin sections of formalin-fixed tissues: trypsin-treated, indirect peroxidase-labelled antibody technique.

Detection of various antigens was performed on the formalin-fixed, paraffin-embedded sections by indirect peroxidase-labelled antibody technique after pretreatment with trypsin digestion. It was revealed that the optimal condition for trypsin digestion of the paraffin sections differed according to the character of antigen and type of tissue, and, under the optimal condition, this method was easily applicable to the regular paraffin sections obtained in the laboratories for routine examinations. This method was also recommended for the detection of the antigens which were usually stained even without trypsin digestion, since it could not only remove the background staining but also uncover the otherwise masked antigen.

Antigens↗

PubMedDate not supplied

Vascular involvement in cutaneous sarcoidosis.

Sarcoidosis is a systemic granulomatous disease with systemic vascular involvement, that is, granulomatous angiitis and microangiopathy. To determine if there is vascular involvement in cutaneous sarcoidosis, we examined 42 skin specimens taken from 32 patients with cutaneous lesions of sarcoidosis. Cutaneous sarcoidosis was prevalent in older females with high serum angiotensin-converting enzyme (ACE) levels. Most skin lesions appeared during the following-up of sarcoidosis. Granulomatous angiitis was present in 12 specimens of sarcoid skin lesions (30.8%). Eight of the 12 specimens showed venous involvement in the dermis. There was no correlation between the incidence of granulomatous angiitis and the gross pattern of cutaneous sarcoidosis. Immunohistochemically, thrombomodulin was negative in the vascular endothelium close to the granuloma or a periphlebitis lesion. Electron microscopy revealed endothelial swelling, luminal narrowing, and basal lamina layering of the basement membrane in the capillaries and venules in the dermis. These findings demonstrated that granulomatous angiitis and microangiopathy coexist in cutaneous sarcoidosis.

Adult↗

PubMedDate not supplied

Fatal strongyloidiasis latent over 42 years in the antineoplastic chemotherapy of a case with malignant lymphoma.

A 66-year-old man with non-Hodgkin lymphoma died of strongyloides hyperinfection following antineoplastic chemotherapy. The patient had a military service in Micronesia in World War II where he was supposed to acquire strongyloidiasis. Here we stress the utility of bronchoscopic examination and importance of patients' lives in tropical climates in immunocompromised patients.

Aged↗

PubMedDate not supplied

Role of smooth-muscle cells and macrophages in cardiac allograft arteriosclerosis in rabbits.

Accelerated coronary arteriosclerosis is one of the major complications in allogeneic cardiac transplantation, despite the significant improvement in immunosuppressive therapy. In this study we sought to clarify the cellular components in cardiac allograft arteriosclerotic lesions with monoclonal antibodies specific to either the muscle cell (HHF-35) or the macrophage (RAM-11). Four kinds of heterotopic transplantations were performed in the rabbit: (1) low cholesterol-fed (n = 5), (2) 1% cholesterol-fed (n = 5), (3) low cholesterol-fed and immunosuppressed (n = 6), and (4) 1% cholesterol-fed and immunosuppressed (n = 6). The donor hearts were removed after cardiac arrest in groups 1 and 2, and at 5 weeks after transplantation in groups 3 and 4. In groups 1 and 2 acute rejection was associated with slightly thickened intimal lesions composed of abundant infiltrating mononuclear cells and sparsely intermingled smooth-muscle cells. In group 3 the arterial intima were moderately thickened with the predominant proliferation of smooth-muscle cells. In group 4 coronary lumina were almost completely occupied with the intimal foam cells, which were derived mostly from macrophages and some from smooth-muscle cells. These data suggest that the proliferation of smooth-muscle cells might be a major factor contributing to arteriosclerosis in chronic cardiac rejection and that long-term exposure to hypercholesterolemia could induce the accumulation of smooth-muscle cells or macrophage-derived foam cells.

Animals↗