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Biomedical subjects

Y Dong

Publications and source records attributed to Y Dong.

At least 253 records · Page 14Linked to original sources
PubMed1995

[The effect of sagittal split ramus osteotomy advancement and setback mandible on occlusal force and masticatory efficiency].

21 patients (mandibular prognathism = 11, mandibular retrognathism = 10) whose occlusal force and masticatory efficiency were measured and analysed preoperatively, three and six months following sagittal split ramus osteotomy. The result shows that, in individuals with mandibular retrognathism treated by lengthening of the mandible, the occlusal force and masticatory efficiency would decrease, in individuals with mandibular prognathism treated by reduction in mandibular length, the occlusal force and masticatory efficiency would increase. There is no linear correlation relationship between the change of the occlusal force and the movement of the angle and the distance of the mandible.

Adult↗
PubMed1995

Prevalence of primary Sjögren's syndrome in China.

OBJECTIVE: To determine the prevalence rate of primary Sjögren's syndrome (SS) in a community population of 2066 adults in a Beijing suburban village, and 100 inpatients, who were not necessarily admitted for rheumatic diseases. METHODS: Questionnaire and serological studies of antinuclear antibodies, rheumatoid factor, and anti-SSA, anti-SSB antibodies were done. Possible positive subjects were given eye and oral examinations for objective evidence of xerostomia and keratoconjunctivitis sicca. RESULTS: According to the Copenhagen criteria, the prevalence rate of primary Sjögren's syndrome in China was 0.77%, and it was 0.33% by modified San Diego criteria. In 100 inpatients, we found 4 cases of primary Sjögren's syndrome by Copenhagen criteria, and one case by modified San Diego criteria. CONCLUSION: Primary Sjögren's syndrome is not a rare disease in China, but most cases are overlooked or misdiagnosed.

Adolescent↗
PubMed1995

[Effect of sex steroids on serum amyloid P-component (female protein) in rats].

Serum amyloid P-component (SAP) has been designated as a female protein in hamsters. But such a distinction is not made for rats. In order to investigate the effects of sex-steroids on the SAP level in rats, SAP was purified from Wistar rats by affinity chromatography of phosphorylcholine, followed by gel filtration. Anti-SAP was raised through the immunization of rabbits with the rat SAP and Freund's adjuvant. Sample sera were obtained from 180 young and old rats, after which rats were injected with estradiol (E2), testosterone (T) or dehydroepiandrosterone (DHEA). Sera were serially obtained from the tail vessels until the 8th day after injection. The SAP level was assayed by micro single radial immunodiffusion. As the rats aged, the SAP levels increased from 2.9 mg/dl at 11 weeks to 10.7mg/dl at 58 weeks. In 37-week-old rats, the SAP levels in females (6.3 +/- 1.8 mg/dl) were significantly (p < 0.001) higher than those in males (3.9 +/- 1.0 mg/dl). The SAP levels did not change after T administration, but were increased rapidly by E2 administration, especially in young male rats (increased from 2.6 +/- 0.2 mg/dl to 4.9 +/- 0.7 mg/dl). The SAP levels were decreased significantly (p < 0.05) by DHEA injection. Serum E2 levels in young (11 wk) male rats were very low before E2 injection, and rose steeply on the 2nd day. From these findings, the different SAP levels in mature female and male rats are attributed to E2.

Aging↗
PubMed1995

Ultrastructural cytochemistry of human gastric cancer: electron microscopic observations of five organellae marker enzymes.

The distribution of ALPase, ACPase, G6Pase TPPase and CCOase of gastric cancer and normal gastric epithelium were studied ultrastructurally. The results showed that normal gastric epithelium had no ALPase reaction. The reactions of ACPase, G6Pase, TPPase and CCOase were found in the corresponding organellae which were consistent with their functions. In tubular adenocarcinoma cells, their reactions were more apparent in the corresponding organellae. Some cells of tubular adenocarcinomas showed ALPase reaction. The mucinous adenocarcinoma cells had higher ACPase and TPPase reactions. In poorly differentiated adenocarcinoma cells, the five marker enzymes showed negative or faint reactions. The biological significance and mechanisms of distribution of the five marker enzymes were discussed.

Acid Phosphatase↗
PubMed1995

[Interspecific protoplast fusion between Bacillus thuringensis Bt-3701 and Bacillus megaterium Bm-107].

The results of the interspecific protoplast fusion between B. thuringensis sub. kurstaki Bt-3701 which has pesticide ability, and B. megaterium var. phosphaticum Bm-107 which has decomposing phosphate activity, were reported. High frequency of protoplast formation and regeneration was obtained with 4h activated Bm-107 treated by 100 micrograms/ml lysozyme, and with 2h activated Bt-3701 treated by 3% glycin and mild temperature. Using 40% PEG and 5% nascent Ca2+ to treat the parential protoplast mixture for 3 min at 37 degrees C, 4 stable fusants were obtained. Biological tests show that they have both pesticide ability and decomposing phosphate activity, but which are weaker than that of parential strains.

Bacillus megaterium↗
PubMed1994

Volume-activated chloride current is not related to P-glycoprotein overexpression.

It has been suggested that P-glycoprotein (P-gp), an ATP-dependent transporter responsible for classical multidrug resistance, is also a volume-regulated chloride channel. We reexamined this hypothesis by use of whole-cell patch clamp recordings of three matched pairs of cell lines, which were either drug-sensitive or drug-resistant due to P-gp overexpression. We demonstrate here that volume-regulated chloride-selective currents can be induced in cells with or without P-gp expression. Overexpression of either P-gp or cystic fibrosis transmembrane conductance regulator, the protein product of the CF gene and another member of the ATP-dependent transporters, is associated with a hypotonicity-induced, rapid onset, transient current prior to onset of the volume-sensitive chloride-selective current, an apparent nonspecific effect related to the overexpression of an integral membrane protein. These results suggest that there is no relationship between P-gp and the chloride channel activated by cell swelling.

3T3 Cells↗
PubMed1994

Isolation and characterization of two replication-dependent mouse H1 histone genes.

Mice contain at least seven nonallelic forms of the H1 histones, including the somatic variants H1a-e and less closely related variants H1 degrees and H1t. The mouse H1 degrees and H1c (H1var.1) genes were isolated and characterized previously. We have now isolated, sequenced and studied the expression properties of two additional mouse H1 genes, termed H1var.2 and H1var.3. Extensive amino acid and nucleotide sequence comparisons were made between the two genes and other mammalian H1 histone genes. A high degree of nucleotide sequence identity was seen between the H1var.2, rat H1d and human H1b genes, even well beyond the coding region, indicating that these genes are likely homologues. Unlike the previously characterized mouse H1var.1 gene which produces both nonpolyadenylated and polyadenylated mRNAs, the H1var.2 and H1var.3 genes produce only typical, replication dependent, nonpolyadenylated mRNAs.

Amino Acid Sequence↗
PubMed1994

Identification of the catalytic base in long chain acyl-CoA dehydrogenase.

We have used molecular modeling and site-directed mutagenesis to identify the catalytic residues of human long chain acyl-CoA dehydrogenase. Among the acyl-CoA dehydrogenases, a family of flavoenzymes involved in beta-oxidation of fatty acids, only the three-dimensional structure of the medium chain fatty acid specific enzyme from pig liver has been determined (Kim, J.-J.P., Wang, M., & Paschke, R. (1993) Proc. Natl. Acad. Sci. U.S.A. 90, 7523-7527). Despite the overall sequence homology, the catalytic residue (E376) of medium chain acyl-CoA dehydrogenase is not conserved in isovaleryl- and long chain acyl-CoA dehydrogenases. A molecular model of human long chain acyl-CoA dehydrogenase was derived using atomic coordinates determined by X-ray diffraction studies of the pig medium chain specific enzyme, interactive graphics, and molecular mechanics calculations. The model suggests that E261 functions as the catalytic base in the long-chain dehydrogenase. An altered dehydrogenase in which E261 was replaced by a glutamine was constructed, expressed, purified, and characterized. The mutant enzyme exhibited less than 0.02% of the wild-type activity. These data strongly suggest that E261 is the base that abstracts the alpha-proton of the acyl-CoA substrate in the catalytic pathway of this dehydrogenase.

Acyl-CoA Dehydrogenase, Long-Chain↗
PubMed1994

Transcriptional regulation of differentiation, selective toxicity and ATGCAAAT binding of bisbenzimidazole derivatives in human melanoma cells.

To study the relationship between the structure of minor groove ligands and their affinity for specific DNA sequences that regulate gene transcription, three analogues of the A-T-specific DNA minor groove ligands Hoechst 33258 and Hoechst 33342 were synthesized with 5, 8 or 12 carbons in an aliphatic chain attached to the phenolic oxygen of the molecule. There was a striking bimodal relationship between toxicity to HeLa cells and the lipophilicity of the five analogues, toxicity being low for the compounds with a free hydroxyl (Hoechst 33258) or a 12-carbon substituent, yet high for the 5-carbon analogue. Selective killing of human melanoma cells compared with normal fibroblasts was observed for the Hoechst analogue with a 12-carbon chain attached. Hoechst 33258 itself was selectively toxic for the MM96E melanoma cell line compared with other cell lines, induced a highly dendritic morphology, increased tyrosinase activity and tyrosinase mRNA but decreased the level of gp75 (TRP-1) mRNA; message for a third pigment gene, Pmel-17, was unchanged. Tyrosinase activity was decreased in the resistant A2058 melanoma cell line and transcription was affected to a lesser extent than in MM96E. Expression of gp75 protein and two intermediate filament proteins was inhibited by Hoechst 33258 in MM96E cells. There was no major difference in the amount of 125I-Hoechst 33258 taken up by sensitive and resistant cells. Of the five derivatives studied, the parent drug Hoechst 33258 and the 2-carbon analogue (Hoechst 33342) were found to have the most inhibitory effect on affinity of octamer binding proteins for the ATGCAAAT consensus sequence found in the promoter region of certain genes associated with proliferation and differentiation. In contrast to Distamycin A (also an A-T-specific minor groove ligand), Hoechst 33258 displaced proteins already bound to the octamer motif. The G-C ligand chromomycin A3 exhibited a different spectrum of cell toxicity and tyrosinase stimulation compared with Hoechst 33258. Chromomycin A3 but not Hoechst 33258, strongly inhibited the zinc-dependent transcriptional activity of the sheep metallothionein-Ia promoter in reporter gene assays of transfected cells. Since the six metal-responsive elements of the promoter are GC-rich, this provides independent evidence for the sequence-specificity of transcriptional inactivation by one of these drugs in melanoma cells. Overall, the results suggest that Hoechst 33258 acts by inhibiting the transcription of specific genes, cell lines evidently differing in the accessibility to drugs of certain A-T-rich sequences.

Base Sequence↗
PubMed1994

The N-glycosidase mechanism of ribosome-inactivating proteins implied by crystal structures of alpha-momorcharin.

BACKGROUND: alpha-Momorcharin (alpha MMC) is a type I ribosome-inactivating protein. It inhibits protein synthesis by hydrolytically removing a specific adenine residue from a highly conserved, single-stranded loop of rRNA. RESULTS: Here we describe the determination and refinement of the crystal structures of alpha MMC in the native state and in complexes with the product, adenine, and a substrate analogue, formycin 5'-monophosphate (FMP) at high resolution. Both adenine and the base of FMP are tightly bound; the ribose of bound FMP adopts a strained, high-energy conformation, which may mimic the structure of the transition state. CONCLUSIONS: These structures indicate that residues Tyr70, Glu160 and Arg163 of alpha MMC are the most critical for catalysis. We propose that the strained conformation of the ribose in the target adenosine weakens the glycoside bond. Partial protonation mediated by Arg163 then facilitates N-glycoside bond cleavage, leading to the formation of an oxycarbonium ion intermediate which is stabilized by the negatively-charged Glu160. Tyr70 adopts subtly different conformations in the three structures implying that it may be important in substrate recognition and perhaps catalysis.

Amino Acid Sequence↗
PubMed1994

Mechanisms of glucocorticoid action in bone cells.

Glucocorticoids play an important role in the normal regulation of bone remodeling; however continued exposure of bone to glucocorticoid excess results in osteoporosis. In vivo, glucocorticoids stimulate bone resorption and decrease bone formation, and in vitro studies have shown that while glucocorticoids stimulate osteoblastic differentiation, they have important inhibitory actions on bone formation. Glucocorticoids have many effects on osteoblast gene expression, including down-regulation of type I collagen and osteocalcin, and up-regulation of interstitial collagenase. The synthesis and activity of osteoblast growth factors can be modulated by glucocorticoids as well. For example, insulin-like growth factor I (IGF-I) is an important stimulator of osteoblast function, and expression of IGF-I is decreased by glucocorticoids. The activity of IGF I can be modified by IGF binding proteins (IGFBPs), and their synthesis is also regulated by glucocorticoids. Thus, glucocorticoid action on osteoblasts can be direct, by activating or repressing osteoblast gene expression, or indirect by altering the expression or activity of osteoblast growth factors. Further investigation of the mechanisms by which glucocorticoids modulate gene expression in bone cells will contribute to our understanding of steroid hormone biology and will provide a basis for the design of effective treatments for glucocorticoid-induced osteoporosis.

Animals↗
PubMed1994

Effects of mastication on digestion of whole cereal grains by cattle.

A study was conducted to evaluate the effects of mastication on the physical breakdown and ruminal digestion of whole cereal grains by cattle. Three Hereford cows (initial BW 557 kg; SD, 17) surgically fitted with ruminal and esophageal fistulas were fed 5.5 kg/d (as-fed basis) of whole barley, corn, or wheat in an experiment designed as a 3 x 3 Latin square. Eating time and chews per kilogram of DM were greater (P < .10) for corn than for barley or wheat. Whole corn was substantially damaged after ingestive mastication, and the majority of kernels were broken into small pieces. Many barley and wheat kernels showed signs of dentition, but most kernels remained intact. Less saliva (P < .10) was added to whole wheat than to barley or corn during ingestive mastication. Cattle fed whole barley spent twice (P < .10) as long ruminating per kilogram of DM as those fed corn, with intermediate times for wheat. Less than 30% of the DM disappeared from unmasticated whole grains during 96 h of ruminal incubation. Ingestive mastication increased DM digestion to 53, 69, and 66% for barley, corn, and wheat, respectively. Mastication alters the kinetics of ruminal digestion of unprocessed cereal grains. Combined with the inherent protein and starch digestibilities of the grain, mastication affects the type and extent of processing required to optimize the utilization of cereal grains by cattle. Unlike barley and wheat, corn kernels are extensively damaged during ingestive mastication, reducing the need for physical processing.

Ammonia↗
PubMed1994

[The abnormalities of the cellular immunity in rheumatoid synovium].

With the technique of immunohistochemical (ABC) method monoclonal antibodies were used to identify the lymphocyte subsets, macrophage and the expression of class II MHC (HLA-DR, HLA-DQ) antigens in the synovium cells from 18 RA patients and 8 patients with osteoarthritis as control. The results showed that the main cellular abnormality at the sublayer of the synovium was the appearance of lymphoid follicles which mainly consisted of the infiltration of T lymphocytes (77.8%).50.6% of them were CD4+, which mainly consisted by of CD45RO+ cells in the rheumatoid synovium on the consecutive sections. The increased ratio of CD4/CD8 in RA patients was significantly higher when compared with that in the controls (2.11 +/- 0.93 vs 0.63 +/- 0.13, P < 0.001). In advanced RA with fibrosis of joints, the ratio tended to decrease and was accompanied with reduction of infiltrated lymphocytes. Compared with T cells CD20+ B lymphocyte not only had a lower percentage (25.2%), but also showed a characteristic picture of locating in the centre of the follicles. The fact that most of the CD4+ T cells was helper memorized lymphocytes with CD4 phenotype of positive TAC(+) (interleukin-2 receptor) and that up to 54.2% was anti-HLA-DR and 54.1% anti-HLA-DQ monoclonal antibodies indicated that these T lymphocytes were activated in vivo. Cells with anti-CD68+ were seen all over the RA synovium. Class II HLA and CD68 molecule were also expressed on the endothelium cells of the small vessels. It is suggested that the activated lymphocytes, macrophages and endothelium cells and their abnormal distribution may indicate the abnormalities of the cellular immunity in rheumatoid synovium.

Adolescent↗
PubMed1994

[Evaluation of posttraumatic hearing loss].

In this study a comprehensive audiometry was applied to detect the hearing loss in 400 patients (531 ears) with difficult-to-test hearing loss induced by ear or skull injury. It was found that the real PTA detectable rate reached 95.29% and the incidence of hearing loss was 82.11%, of which 68.81% were related to injury. The incidence of nonorganic deafness was 77.59%, i.e., the incidence of hearing loss exaggerated by patients was 57.25% (tested ears), simulated deafness was 16.20% and functional deafness was 4.14%. The principle of dealing with nonorganic deafness was discussed.

Adolescent↗
PubMed1994

Differential expression of two gap junction proteins in corneal epithelium.

Two distinct gap junction proteins (connexins) are expressed in rat corneal epithelium in a way which parallels cellular differentiation processes in this tissue. Connexin43 is restricted predominantly to the basal cells of the corneal epithelium and is present in significantly reduced amounts compared to the situation in the adjacent conjunctival epithelium. In contrast, a gap junction protein recognized by antibodies against MP70 which is the ovine homolog of mouse connexin50, is strongly expressed in the corneal epithelium and is present in the basal cells, wing cells and surface cells. While the functional significance of this differential expression of corneal epithelial connexins has yet to be established, the corneal epithelium is the third avascular tissue besides lens and heart valves which expresses a gap junction protein recognized by anti-MP70 antibodies.

Animals↗
PubMed1994

[Effect of intracerebroventricular injection of norepinephrine and serotonin on somatosensory evoked potentials in rabbits].

The components of somatosensory evoked potentials (SEP), P7, N9, P12, and N15, were recorded from dura mater before and after intracerebroventricular injection (icv) of monoamines. The peak latencies of P12 and N15 were prolonged and the amplitudes of P12 and N15 were decreased following icv norepinephrine (NE) 1 microgram.microliter-1 and serotonin (5-HT) 1 microgram.microliter-1. Generally, P12 and N15 restored to normal 1-2 h after icv NE and 5-HT. Dopamine (DA) 1 microgram.microliter-1, however, did not show any significant effect on SEP. Tolazoline (Tol) 2.5 micrograms.microliters-1 markedly potentiated the inhibitory effect of NE, but propranolol (Pro) 1 microgram.microliter-1 weakened the effect of NE on SEP. These results indicated that icv NE and 5-HT exerted an inhibitory effect on the somatosensory afferent function from thalamus to cortex and/or intercortical areas and alpha-adrenoceptor had an influence on the inhibitory effect of NE.

Animals↗
PubMed1994

[Longitudinal surveillance of chloroquine resistance of Plasmodium falciparum after cessation of medication in south Yunnan].

The resistance of P. falciparum to chloroquine was assayed by in vitro microtest in 1981 and after cessation of medication in 1984, 1988-1990 and 1992 in south Yunnan Province of China. The resistance rates were 97.4% (38/39), 100% (25/25), 96.1% (73/76) and 93.7% (59/63), respectively; ID50 were 170, 132, 125 and 110 nmol/L; ID95 were 1,000, 740, 707 and 576 nmol/L; mean dosages for complete inhibition of schizont formation were 55.4, 46.7, 45.8 and 35.4 (pmol/well). A comparison of the results in 1981, 1984, 1988-1990 and 1992, the resistance rate showed no marked change, ID50 dropped 22.3% (P < 0.05), 26.4% (P < 0.05) and 35.3% (P < 0.01); ID95 declined 26.0% (P < 0.05), 29.3% (P < 0.05) and 42.2% (P < 0.01); mean dosage of inhibition dropped 15.7% (P > 0.05), 17.3% (P < 0.05) and 36.1% (P < 0.01). The results show that the extent of resistance in chloroquine-resistant P. falciparum to chloroquine in south Yunnan has been declined markedly, indicating that coresistance was variable and dropped along with the declining of drug pressure.

Animals↗