Biomedical subjects

Y Chen

Publications and source records attributed to Y Chen.

At least 937 records · Page 52Linked to original sources

PubMed2000

Effect of all-trans retinoic acid on growth of xenograft tumor and its metastasis in nude mice.

OBJECTIVE: To study the effect of all-trans retinoic acid on growth of xenograft tumor and its metastasis in nude mice. METHODS: Human gastric cancer BGC-823 and MKN-45 cells were inoculated into spleen subcapsule of nude mice, respectively. The nude mice were subsequently administered with all-trans retinoic acid every other day. Food consuming and body weight of nude mice were measured weekly. Six weeks later, the nude mice were killed. Xenograft tumors in spleen and metastatic tumors in liver were pathologically examined. Microvessel density in the tumors was detected immunohistochemically, and serum carcinoembryonic antigen was measured by radioimmunoassay. RESULTS: After the nude mice were fed with all-trans retinoic acid, the growth of splenic tumor and its liver metastasis were inhibited and the metastatic rates decreased by 50% (BGC-823) and 33.3% (MKN-45), respectively. The microvessel density in splenic and hepatic tumors reduced by 28.58% and 35.47% (BGC-823), 19.45% and 14.52% (MKN-45), respectively. The concentration of carcinoembryonic antigen decreased by 50.24% (BGC-823) and 48.10% (MKN-45). CONCLUSION: All-trans retinoic acid may effectively inhibit the growth of xenograft tumor in spleen and its metastasis to liver in nude mice, which can be corroborated by the decrease of carcinoembryonic antigen and microvessel density.

Animals↗

PubMed2000

Recombinant bovine basic fibroblast growth factor accelerates wound healing in patients with burns, donor sites and chronic dermal ulcers.

OBJECTIVE: To evaluate the effect of recombinant bovine basic fibroblast growth factor (rbFGF) on accelerating healing of burn wounds, donor sites and chronic dermal ulcers in multicenters. METHODS: One thousand and twenty-four patients with burns, donor sites and chronic dermal ulcers were recruited for this prospective and multicenter clinical trial and another 641 patients with the similar wounds were used as controls. All patients were divided into three groups: burn group, donor site group, and chronic dermal ulcer group. In the burn group, 654 patients with superficial second-degree burns(superficial II degree) or deep second-degree burns(deep II degree) were treated topically with either recombinant bovine basic fibroblast growth factor (rbFGF plus vehicle, rbFGF 150 AU/cm2/day, 330 patients) or placebo (vehicle without rbFGF, 324 patients). In the donor site group, 509 patients were treated with rbFGF and 317 patients with placebo in a similar way as described elsewhere. In the chronic dermal ulcer group, 185 patients were treated with rbFGF and the controls were themselves. Photography, histological evaluation and clinical examination were used to evaluate the results of wound healing. RESULTS: All of superficial second-degree burns, deep second-degree burns and the donor sites treated with recombinant bovine basic fibroblast growth factor had an accelerated rate of granulation tissue formation and epidermal regeneration as compared with that in the controls. Superficial second-degree burns and deep second-degree burns treated with recombinant bovine basic fibroblast growth factor healed in 9.89 +/- 2.45 and 17.04 +/- 4.56 days, respectively, whereas the average healing time in those receiving placebo was 12.35 +/- 2.74 and 21.21 +/- 4.88 days. The donor sites treated with rbFGF or placebo healed in 10.68 +/- 4.55 and 14.74 +/- 6.46 days, respectively. All chronic dermal ulcers except 12 patients treated with rbFGF healed within 6 weeks. Histological evaluation of punch-biopsy specimens from the burn wounds, donor sites and chronic dermal ulcers seven days after treatment supported the impression of accelerated wound healing after rbFGF treatment. CONCLUSIONS: rbFGF is effective in shortening the wound healing time and improving the wound healing quality. Accelerating wound healing is of clinical benefit in reducing the length of hospitalization, costs and emotional burden of the patient.

Adolescent↗

PubMed2000

CT via percutaneous splenoportography: experiment and application.

OBJECTIVE: To introduce computed tomography via percutaneous splenoportography (CTSP), a new method for determining hepatic diseases. METHODS: Ten hybrid dogs and 31 patients with primary hepatic cancer (PHC) were included in the study. Each dog was examined by CT, computed tomography via arterial portography (CTAP), and CTSP to compare the enhanced density of the liver. The 31 patients were examined by CTSP and the appearance of PHC was compared with their pathological results to evaluate the diagnostic significance of CTSP. RESULTS: The animal experiment showed that both CTAP and CTSP could obviously enhance the density of the liver (P < 0.01), but no significant difference was observed in the enhanced densities between the two methods (P > 0.05). On the CTSP images of the 31 patients, the density of the livers was increased to 168-192 Hu, whereas the density of the cancers was as low as that on the images of CT scans (< 58 Hu). The CTSP findings were consistent with the surgical ones from space occupying lesions. The diagnostic value of CTSP was obviously superior to that of general enhanced CT. However, it was difficult for CTSP to show nodules less than 1 cm in size located on the surface of the liver or the hepatic portal zone. CONCLUSIONS: Like CTAP, CTSP is also a sensitive method for showing occupant in the liver. But the equipments and the procedures are simpler for CTSP than for CTAP. CTSP is an alternative procedure in clinical practice.

Animals↗

PubMed2000

P16 overexpression in pituitary adenomas studied by immunohistochemistry and in situ hybridization.

OBJECTIVES: To examine the expression of p16 gene and the clinicopathological characteristics of 31 pituitary adenomas. To investigate the relationship between p16 expression and cell proliferative activity by analyzing the expression of proliferating cell nuclear antigens (PCNA). METHODS: Immunohistochemistry and in situ hybridization were performed on freshly-prepared paraffin-embedded specimens of 31 pituitary adenomas. RESULTS: Varying degrees of overexpression of the p16 gene was found in the tumors specimens. A higher proportion of p16 positive tumor cells was associated with bigger size, more frequent invasion and higher recurrence of the tumors. The expression of p16 was found to be significantly associated with the degree of PCNA expression. CONCLUSION: Though inactivation of the p16 gene is known in wide range of tumors, our study suggested that p16 overexpression might play a role in the development and progression of pituitary adenomas.

Adenoma↗

PubMed2000

[Rhubarb extracts in treating complications of severe cerebral injury].

OBJECTIVE: To investigate the therapeutic effects of the Chinese medicinal herb, Rhubarb, on severe brain injury. METHODS: Rhubarb extracts in ethyl alcohol and water were used to treat 20 patients with severe cerebral injury complicated by hyperthermia, renal failure, hemorrhage in the upper digestive tract, and increased intracranial pressure. Vital signs, variations of Glasgow coma scale (GCS) and intracranial pressure (ICP) of the patients were observed. The degree of hemorrhage in the digestive tract and the change in creatinine value were also observed. Other 20 patients served as controls. RESULTS: The result of the treatment group was more marked than that of the control group. Three days after administration of the rhubarb extract, the body temperature decreased by 1.1 degrees C, ICP by 0.4 kPa (1 mm Hg = 0.1333 kPa) on average, and the incidence of hemorrhage in the digestive tract by 30%. The volume of hemorrhage of the digestive tract decreased by 700 +/- 250 ml, and the time for turning occult blood to negative by 10%. CONCLUSION: The Chinese medical herb, rhubarb, has multiple therapeutic effects on severe brain injury.

Adolescent↗

PubMed2000

Acute fatty liver of pregnancy: diagnosis and management of 8 cases.

OBJECTIVE: To investigate the early recognition and management of acute fatty liver of pregnancy (AFLP) to improve maternal and fetal survival. METHODS: Eight cases of AFLP seen in our hospital during the past three years were studied retrospectively. Symptoms, laboratory findings, timing of liver biopsy, and maternal and fetus outcome were assessed. RESULTS: The mean gestational age at onset was 34 +/- 2 weeks. All cases were primigravida. In the early stages, all patients presented malaise, nausea, vomiting and epigastric distress followed by jaundice in the third trimester of pregnancy. LABORATORY FINDINGS: all had raised transaminases and serum bilirubin (32.5-510.8 mumol/L), hypoalbuminemia (22.4-30.0 g/L), hypofibrinogenemia (< 180 mg/dl), prolonged prothrombin time and prolonged partial thromboplastin time. Maternal complication included hepatic encephalopathy (6 cases), ascites (6), hypoglycemia (5), hematemesis (2), and postpartum hemorrhage (5) and preeclampsia (4). Emergency cesarean section was performed in 3 cases. One mother died of fulminant hepatic failure and the others survived. There was no fetus death. Liver biopsy was done on the 5th to 15th postpartum day in 8 cases. CONCLUSION: With increasing awareness, especially in the early recognition of milder cases, and prompt progressive management including early termination of pregnancy by cesarean section and large dose infusion of fresh frozen plasma and albumin alternately, the prognosis of AFLP can be improved.

Acute Disease↗

PubMed2000

[Effects of ovulation induction therapies on endometrial integrin alpha v beta 3 expression in patients with polycystic ovarian syndrome related infertility].

OBJECTIVE: To study the endometrial integrins alpha v beta 3 expressions during mid-luteal(ML) phase in polycystic ovarian syndrome (PCOS) related infertile patients receiving ovulation induction therapies. METHODS: Immunohistochemical methods using monoclonal antibody against integrins alpha v beta 3 were performed on endometrium of ML phase obtained from 22 normal subjects and 40 PCOS related anovulatory patients who underwent ovulation induction therapy by one of the following regimens: clomiphene citrate (CC) + human chorionicgonadotropin (hCG), CC + human menopausal gonadotropin (hMG) + hCG, or gonadotropin-releasing hormone agonist + hMG + hCG. RESULTS: The expressions of endometrial integrin alpha v beta 3 during ML phase in GnRHa + hMG + hMG + hCG cycles were similar with those in normal natural cycles, but were weaker in CC + hCG, CC + hMG + hCG cycles as compared with controls. CONCLUSION: CC + hCG, CC + hMG + hCG regimens may induce decline of endometrial receptivity in ML phase.

Adult↗

PubMed2000

[Study on the relationship between a polymorphism of tumor necrosis factor-alpha gene and the pathogenesis of polycystic ovary syndrome].

OBJECTIVE: To investigate the relationship between -308 bp polymorphism in tumor necrosis factor-alpha (TNF-alpha) gene promoter region and the pathogenesis of polycystic ovary syndrome (PCOS). METHODS: One hundred eighteen Chinese women with PCOS (44 were obese, and 74 nonobese) and 54 controls were genotyped according to the fragment length [87 bp and(or) 107 bp] of TNF-alpha gene promoter by the technique of polymerase chain reaction-restriction fragment length polymorphism. The distribution of TNF1 genotype (87/87) and TNF2 genotype (87/107 + 107/107) in the two groups and their relations to parameters of glucose metabolism associated with PCOS were compared. RESULTS: There was no significant difference in the distribution of TNF1 and TNF2 genotypes between PCOS group and the controls (P > 0.05). In obese PCOS group, only significant lower insulin sensitivity index was found in TNF-1 genotype as compared with TNF2 genotype (P < 0.05). In nonobese PCOS group, there was no significant difference in all parameters of glucose metabolism between TNF1 and TNF2 genotype. All parameters of glucose metabolism in obese patients with TNF1 genotype were significantly different from those in nonobese with TNF1 genotype. However, significant differences were found only in some parameters when comparison was made between obese and nonobese within the TNF2 genotype group. CONCLUSION: There is no association between TNF-alpha gene -308 bp polymorphism and the pathogenesis of polycystic ovary syndrome, but PCOS patients with TNF2 genotype may associate with improvement to certain degree on abnormal glucose tolerance usually existed in obese PCOS women.

Adult↗

PubMed2000

E test for studying in vitro activity of seven antimicrobial agents against penicillin-susceptible and penicillin-resistant pneumococci.

OBJECTIVE: To evaluate the comparative activity of seven oral antimicrobial agents against 100 strains of Streptococcus pneumoniae (S. pneumoniae). METHODS: Total 100 strains of S. pneumoniae were collected from general hospitals in Beijing from June 1996 to June 1997. E test method was used to detect the minimum inhibitory concentration (MIC) values of the following seven drugs against S. pneumoniae: penicillin, amoxicillin/clavulanate, cefaclor, cefuroxime, ceftriaxone, azithromycin and ofloxacin. The breakpoint of susceptibility categories was defined according to NCCLS. RESULTS: 74% of isolates were susceptible (S, MIC 0.06 mg/L) to penicillin, and 5% were penicillin resistant (R, MIC 2 mg/L). The intermediately resistant (I, MIC 0.1-1 mg/L) rate to penicillin was 21%. The total rates of R or I were 26% for penicillin, 6% for both amoxicillin/clavulanate and ceftriaxone, 9% for cefuroxime, 66% and 68% for azithromycin and ofloxacin respectively. Overall 94%, 94% and 91% of the pneumococcal isolates were susceptible to amoxicillin/clavulanate, ceftriaxone and cefuroxime respectively. S. pneumoniae was highly resistant to azithromycin tested as a representative of macrolides (MIC50 and MIC90 both > 256 mg/L, R rate 66%), and had a highly intermediately resistant rate to ofloxacin (I rate 62%). CONCLUSIONS: Our data suggest that in vitro activity of oral amoxicillin/clavulanate was as good as ceftriaxone and cefuroxime. It can be considered as an alternative compound in the treatment of community acquired pneumoniae and other respiratory tract infections caused by multiresistant S. pneumoniae.

Amoxicillin↗

PubMed2000

Activated protein C resistance in antiphospholipid thrombosis syndrome.

OBJECTIVE: To explore the correlation between antiphospholipid antibodies (APA), activated protein C resistance (APCR) and antiphospholipid thrombosis (APL-T) syndrome and further investigate the mechanism of thrombosis in APL-T syndrome. METHODS: ELISA, PTT-LA and APTT +/- APC methods were used to detect anticardiolipin antibodies (ACA), lupus anticoagulants (LA) and APC R in 20 APL-T syndrome patients. RESULTS: Twenty patients were diagnosed as APL-T. ACA-IgG, M and LA are strongly associated with APL-T. The incidence of APCR in APL-T (75%) was significantly higher than that of the normal group (5%). CONCLUSION: There was high prevalence of APCR in APL-T, which was strongly associated with LA. It is proposed that acquired APCR induced by APA is a key to understanding thrombosis in Chinese APL-T patients.

Activated Protein C Resistance↗

PubMed2000

A randomized controlled clinical trial on etimicin, a new aminoglycoside antibiotic, versus netilmicin in the treatment of bacterial infections.

OBJECTIVE: To compare the efficacy and safety of two aminoglycoside antibiotics, etimicin and netilmicin, in the treatment of bacterial infections. METHODS: A randomized, open label, controlled clinical trial was conducted for the treatment of 65 patients hospitalized with respiratory tract infections, urinary tract infections, and skin and tissue infections. Thirty-four patients received etimicin and thirty-one patients received netilmicin at a dose of 100 mg every 12 hours by intravenous infusion. The duration of treatment was 7-10 days in both groups. RESULTS: 47 patients were enrolled in the etimicin group; 35 patients were assessable for safety and 34 patients were assessable for efficacy, 46 patients were enrolled in the netilmicin group; 32 patients were assessable for safety and 31 patients were assessable for efficacy. The results show that overall efficacy was 85.3% for the etimicin group and 83.9% for the netilmicin group, whereas bacterial clearance rates were 87.5% for the etimicin group and 89.7% for the netilmicin group. The incidence of adverse reactions was 8.6% (3/35) and 9.4% (3/32), respectively. CONCLUSION: Etimicin and netilmicin were effective and safe for the treatment of respiratory tract infection, urinary tract infection, and skin and tissue infections. The results show there was no statistically significant difference between the two groups (P > 0.05).

Adolescent↗

PubMed2000

[Detection and significance of interleukin-8,6, tumor necrosis factor-alpha in sputa from patients with chronic obstructive pulmonary disease].

OBJECTIVE: To evaluate the role of cytokines in the pathogenesis of COPD. METHODS: Induced sputum samples were obtained from 20 patients with stable COPD, 10 healthy smokers (HS) and 10 healthy nonsmokers (HNS). Total and differential cell counts were examined, and sputum interleukin (IL)-8, tumor necrosis factor (TNF)-alpha, IL-6 level were measured by radioimmunoassay technique. RESULTS: Sputum IL-8, TNF-alpha level and neutrophil percentages (PMN) in the COPD group were significantly higher than those in the HS group and the HNS group (P < 0.01). Compared with the HNS group, there was a significant increase in the PMN and the level of IL-8 in the HS group (P < 0.05). The sputum level of IL-6 did not differ significantly among three groups (P > 0.05). In the COPD group, the sputum level of IL-8 was correlated positively with the level of TNF-alpha and the PMN (r's = 0.5999, 0.6737). In the HS group the sputum level of IL-8 was also correlated positively with the PMN (rs = 0.8424). The sputum IL-8, TNF-alpha levels and the PMN were all correlated negatively with FEV1% and FEV1/FVC in the COPD group (r's = -0.6453, -0.7341, -0.7341, -0.7624, -0.5262, -0.7353). CONCLUSIONS: IL-8, TNF-alpha and neutrophil are involved in airway inflammation which results in airflow obstruction, thus playing an important role in the pathogenetic process of COPD.

Aged↗

PubMed2000

[The diagnostic value of telomerase activity in bronchial biopsy specimen for lung cancer].

OBJECTIVE: To explore the diagnostic value of telomerase activity for lung cancer. METHODS: Telomerase activity was measured by PCR-TRAP in cancerous lesions and contralateral bronchial tissues from patients with lung cancer and in the bronchial tissues from patients with noncancerous pulmonary disorders, in which the tissue specimens were obtained by fiberoptic bronchoscopy. The result of telomerase detection was compared with the positive rates of pathologic study and bronchial brush cytology. RESULTS: The positive rate of telomerase activity in lesions of lung cancer was significantly higher than that in tissues of the contralateral bronchi from patients of lung cancer (P < 0.01) and that in tissues from patients of noncancerous pulmonary disorders (P < 0.01). There was no significant difference between the positive rate of telomerase activity expression and the positive rates of pathology and bronchial brush cytology (P > 0.05). CONCLUSION: Examination of telomerase activity was of significance in the diagnosis of lung cancer.

Biomarkers, Tumor↗

PubMed2000

[The changes of guanylin in plasma and lung tissue from asthmatic guinea pigs].

OBJECTIVE: To study the changes of guanylin in asthmatic guinea pig models. METHODS: Male guinea pigs were randomly divided into two groups: the control (n = 6) and the asthma group (n = 10). The levels of guanylin in plasma, BALF and lung tissue were measured by RIA kit. The mean thickness of airway wall and glands was determined by contour tracing, using a digitizing pad and microcomputer. RESULTS: The levels of guanylin in plasma and lung tissue from the asthma group were 56% and 183% higher than those of the control (P < 0.01). Concentrations of guanylin in BALF from the control were below the detection level in 4 guinea pigs, and 1.7 pg/ml and 1.6 pg/ml in two, respectively. The level of guanylin in BALF from asthma group was (3.1 +/- 1.2) pg/ml. The level of guanylin in the lung tissue correlated with that in plasma and BALF (r = 0.72, P < 0.01, r = 0.61, P < 0.05, respectively). The level of guanylin in plasma did not correlate with those of BALF (r = 0.32, P > 0.05). The mean thickness of airway wall and glands in asthma group were 86% and 87% greater than those of the control respectively (P < 0.01). The level of guanylin in lung tissue correlated with the thickness of glands (r = 0.95, P < 0.01). CONCLUSIONS: In the guinea pig model of asthma, guanylin in plasma, BALF and lung tissue were increased, indicating that it may play a role in the mechanism of airway hypersecretion in asthma.

Animals↗

PubMed2000

[Effects of PML and PML-RAR alpha antisense oligonucleotides on promyelocytic leukemia cell line NB4].

OBJECTIVE: To investigate the different effects of anti-PML (promyelocytic leukemia) and anti-PML/RAR alpha (promyelocytic leukemia/retionic acid receptor alpha) antisense oligonucleotides on cell growth, expression of PML-RAR alpha mRNA and PML-RAR alpha/PML protein location of NB4 cell line. METHODS: RT-PCR was used for PML-RAR alpha mRNA expression, trypan blue exclusion for cell count, methylcellulose assay for leukemic colony forming unit, immuno-fluorescence for PML-RAR alpha/PML protein localization. RESULTS: Both anti-PML start codon region antisense (STAS) and anti-PML-RAR alpha fusion region antisense (FUAS) could inhibit cell growth and formation AML-CFU. Cells became partially differentiated on day 5, being more marked in FUAS-treated cells than in STAS-treated ones. Down regulated PML-RAR alpha mRNA expression occurred at 24 h was in STAS and FUAS-treated cells and maintained for up to 72 h. Immuno-fluorescence analysis with anti-PML monoclonal antibody showed a remarkable decrease to almost complete disappearance of microgranules. The residual granules became enlarged to become discrete dots (< 10 per cell), similar to normal POD structure in some STAS-treated cells at 24 h. AT 72 h, nearly all the granules disappeared. Similar changes were observed in FUAS-treated cells. CONCLUSION: Both PML and PML-RAR alpha antisense oligonucleotides can specifically block the expression of PML-RAR alpha at mRNA and protein levels. PML protein is implicated in the regulation of cell differentiation.

Cell Division↗

PubMed2000

[Imaging findings of intrahepatic peripheral cholangiocarcinoma].

OBJECTIVE: To study the imaging features of intrahepatic peripheral cholangiocarcinoma. METHODS: The imaging features of 11 cases of surgically and pathologically confirmed intrahepatic peripheral cholangiocarcinoma were retrospectively reviewed. B-ultrasonic scanning(BUS) was performed in all patients. CT was done in 8 patients, including conventional unenhanced scanning followed by enhanced scanning in 4 patients, dynamic double-stage scanning in 2 patients, and dynamic double-stage scanning followed by delayed scanning in the other 2 patients. Of the 3 patients received MRI, conventional SE series, T1WI and T2WI were performed in 2 patients, conventional SE series and dynamic scanning in the other one. RESULTS: All lesions were hypo-echoic in BUS, homogeneous or heterogeneous. On unenhanced CT, the lesions were of low density with ill-defined border. On enhanced scanning, moderate enhancement was observed at the edge of the lesions. MRI was of low intensity in T1WI and moderately high intensity in T2WI. Dynamic MRI and double-stage CT scanning showed characteristic enhancement from the periphery toward the center of the lesion. Focal dilatation of the intrahepatic bile ducts around the tumor was seen in 6 cases and retraction of liver capsule in 3 cases. CONCLUSION: To some extent, the imaging features of intrahepatic peripheral cholangiocarcinoma are characteristic. They are useful for differentiating these tumors from other space-occupying lesions in the liver.

Aged↗

PubMed2000

[The influence of malocclusion on self-esteem and personality of college student].

OBJECTIVE: To study the relationship among the different malocclusions and self-esteem and personality. METHODS: Two hundred and forty college students with normal occlusion and different types of malocclusion were classified into four groups(class I, class II, class III and normal) by Angle's classification, Sixty students in each group. The first three groups had been divided into three subgroups respectively by IOTN: mild, moderate and severe, twenty students in each subgroup. Each subject was assessed by using the body-esteem scale (BES) and the Eysenck personality questionnaire (EPQ). RESULTS: (1) the mean score on facial appearance subscale in BES of the normal group is significantly higher than that of moderate and severe subgroups in class II and class III groups; (2) the mean T score on E subscale in EPQ of the normal group is significantly higher than that of severe subgroups in class II and class III groups, and the mean T score on N subscale in EPQ of the normal group is significantly lower than that of severe subgroups in class II and class III groups; (3) There is a positive correlation between the mean score on facial appearance subscale in BES and the mean T score on E subscale in EPQ and a negative correlation between the mean score on facial appearance subscale in BES and the mean T score on N subscale in EPQ. CONCLUSIONS: (1) The students with moderate and severe class II and class III malocclusion have lower self esteem about their facial appearance than normal students; (2) The trends of introverstion and astatic emotion are higher in students with severe class II and class III malocclusion than in normal students; (3) The higher self esteem about his (or her) facial appearance, the higher trends of extroversion and statical emotion he(or she) has; the lower self-esteem about his (or her) facial appearance, the higher trends of introversion and astatic emotion he (or she) has.

Adult↗

PubMed2000

[Exploration of weight-reducing effect of Kintop in obese rats and its mechanism].

OBJECTIVE: To investigate the weight-reducing effect of Kintop and its probable mechanism in obese rats. METHODS: Body weight, Lee's index, total weight of celiac fat tissue, adipocyte size, blood glucose, blood lipid, expression of leptin in adipocyte (by ABC method), level of serum leptin (by RIA) of obese rats were chosen as the indexes to compare the weight-reducing effect of different dosages of Kintop. The administration of Kintop lasted 1 month. RESULTS: Good effect could be obtained by administration of medium dose Kintop (40 mg/100 g body weight per day) in simple obese rats. At the same time of reducing body weight, blood cholesterol and sugar levels also decreased in a certain degree. The adipocyte leptin expression reduced and serum leptin elevated in those rats with body weight reduced effectively by Kintop. CONCLUSION: Medium dose of Kintop administration for 1 month shows evident weight-reducing effect in simple obese rats, the effect might be related to raising the serum leptin level. Kintop also has the effect in lowering blood cholesterol level, and it could lower the blood sugar in obesity complicated with hyperglycemia by increasing the dosage appropriately.

Animals↗