Search PubMed⌕ Search

Biomedical subjects

Y Chardonnet

Publications and source records attributed to Y Chardonnet.

At least 91 records · Page 5Linked to original sources
PubMed1983

Identification of shared antigenic determinants of different polypeptides from Davis and Towne cytomegalovirus strains with monoclonal antibodies.

Six colonies producing antibodies were obtained by fusing mouse myeloma SP2-O cells with spleen cells from mice immunized with cytomegalovirus Davis strain. Among 88 surviving clones, 29 produced antibodies detectable by immunofluorescence on infected MRC5 cells and 2 others produced neutralizing antibodies against a homologous virus. Supernatants from these 31 positive clones and 4 others which were negative in immunofluorescence or neutralization were tested for their capacity to bind polypeptides from labelled Davis-infected cell extracts. Only 8 were found to be positive: five clones (A8, E3, F2, F8 and F20) precipitated 76 K, 60 K and 54 K bands; three others (A4, B9 and C24) precipitated 76 K and 54 K only. Surprisingly, these 8 monoclonal antibodies recognized a unique polypeptide 67 K of Towne-infected MRC5 cells. No correlation was found between (1) the pattern of fluorescence on MRC5 cells infected with Davis strain, (2) the neutralizing activity, and (3) the polypeptides recognized by the monoclonal antibodies.

Animals↗
PubMed1983

T-cell subsets and Langerhans cells in wart lesions.

The presence of T-lymphocytes and Langerhans cells was assessed on tissue sections of 28 human warts from various localizations and in normal epidermis by indirect immunofluorescence using specific monoclonal antibodies. In most cases, the density of Langerhans cells was dramatically reduced in wart epidermis as compared to normal epidermis and a mild cellular infiltrate could be noted. In some lesions, OKT4 and OKT8 cell populations were present and the number of Langerhans cells was high both in dermis and epidermis. These data are compatible with the role of Langerhans cells and the existence of a local cellular immune reaction in human papilloma virus-infected tissues. No correlation could yet be established with the virus type on the skin localization.

Adolescent↗
PubMed1982

Antigenic polypeptides of 3 reference strains recognized by sera from cytomegalovirus (CMV) infected patients.

The polypeptides of Cytomegalovirus (CMV) from 3 references strains (Davis, Ad 169 and Towne) were precipitated by immune sera from 5 renal transplanted patients with CMV-re-infection and 2 sera from patients with primary infection (one renal transplant and one child). Two antibody negative sera (tested in complement fixation test and immunofluorescence) were used as controls. The patterns of antigenic late polypeptides depended on the kinetics of in vitro infection. They differed between the 3 reference strains, and, for each strain, between the immune sera used for precipitation. Only 6 major bands were recognized by all sera in all reference strains. These results demonstrated he heterogeneity of CMV antigenic polypeptides responsible for antibody production in individual patients.

Antigens, Viral↗
PubMed1982

Detection of virus-specific IgA antibodies in serum of kidney transplant patients with recurrent cytomegalovirus infection by enzymeimmuno and radioimmunoassay techniques.

The feasibility of using human cytomegalovirus (CMV)-specific IgA antibody determinations as a signal for early detection of recurrent CMV infections in eight renal transplant recipients was analyzed. Solid phase radioimmunoassay (RIA), enzyme-liked immunosorbent assay (ELISA) and immunoperoxidase assay (IPA) techniques were used for IgA antibody determinations. In parallel, IgG antibodies to CMV were studied by immunoperoxidase assay. A significant rise of CMV-specific IgG antibody titre was observed in all of these patients between 5 and 53 weeks post-transplantation. CMV-specific IgA antibody production was detected close to the time a rise in CMV IgG antibody was observed in seven out of eight patients studied by RIA and ELISA, and in six out of eight patients studied by IPA. In two patients specific CMV IgA antibodies were detected by all three methods before a significant rise of CMV IgG antibody titre was demonstrated. In these patients CMV IgA was detected by RIA earlier than by ELISA and IPA. The potential application of CMV-specific IgA antibody determination for early detection of recurrent CMV infection in renal transplant patients is discussed.

Adult↗
PubMed1982

Changes in human adenovirus 5 propagated in Burkitt's lymphoma cells.

The effect of host cells on human adenovirus 5 properties was studied with the use of Burkitt's lymphoma cell lines (Raji and Jijoye) that poorly replicate the virus. Only a small fraction of the cell population was producing adenovirus 5. The major virus components were synthesized; however, the purified virus particles differed from those produced in HeLa cells by lower density in CsCl gradient, lower content in DNA, limited changes in DNA restriction enzyme pattern, and polypeptide composition. After one passage in HeLa cells, modifications of polypeptides reversed.

Adenoviruses, Human↗
PubMed1981

Polypeptides and major antigens of four new isolates of cytomegalovirus.

Two strains of cytomegalovirus (CMV) from patients with renal transplants and two other strains of CMV from young children were successfully propagated in human diploid cells, MRC5. Analysis by SDS-PAGE of these viruses passaged only 4 to 6 times revealed 21 to 29 polypeptides. The freshly isolated strains differed from reference strains, Ad 169 and Davis, in the number of bands, in the intensity of labeling of each band and also in molecular weight of the polypeptides. Some bands were common to all six strains. Some bands of molecular weight above 105 K were present in some strains and not in others. This was also the case for some bands between 64 and 76 K and two to five bands at low molecular weight between 17 and 22 K were detected. The band at 68 K was the most intensely labeled. Several polypeptides were precipitated by immune sera containing antibodies to CMV (one positive human serum and one guinea pig antiserum to Davis strain). Different bands were observed after precipitation, depending on the antiserum used and the strain of virus. Interstrain differences were found to be associated with antigenic heterogeneity. However, these cannot be correlated as yet with primary infection or reactivation.

Adult↗
PubMed1981

Detection of specific IgA antibodies in serum of kidney transplant patients with recurrent cytomegalovirus infection.

59 sera of 10 immunosuppressed renal allograft recipients who experienced recurrent cytomegalovirus (CMV) infection were analyzed by enzyme-linked immunosorbent assay (ELISA) for CMV IgA antibodies and by the complement-fixation (CF) test. A significant rise of CF titer was evident 4-53 weeks post-transplantation. 9 patients produced CMV IgA in high titers at about the time of CF antibody rise was observed. 1 patient did not produce IgA antibodies to CMV. In 3 of the 9 patients, specific CMV IgA antibodies were detected before the rise if CF titer was demonstrated. CMV IgA antibodies were found to persist for as long as 6 weeks post-transplantation. The potential application of ELISA detection of CMV-specific IgA antibodies as an early indication of CMV infection in kidney transplant patients is discussed.

Adult↗
PubMed1980

Properties of human adenoviruses 5 and 7 grown in guinea-pig cells.

Human adenoviruses (Ad) 5 and 7 multiplied poorly in guinea-pig cell cultures as compared to HeLa cells. Ad 7 had a modified polypeptide composition with a major 43K component. This change persisted for up to 4 passages in HeLa cells. Ad 5 was not changed by passage in guinea-pig cells.

Adenoviruses, Human↗
PubMed1980

Properties of simian adenovirus 7 after one single passage in simian marmoset lymphoblastoid cells transformed by Epstein Barr virus.

Simian adenovirus 7 gave an abortive infection in simian marmoset lymphoblastoid cells, B 95-8 and M 81 (transformed by Epstein Barr Virus) whereas non transformed simian lymphocytes could not replicate this virus. Electron dense incomplete particles with a lower density than standard virus in CsCl gradients were isolated. Virus yields were low and the percentage of cells containing viral antigen as measured by immunofluorescence was 0.01% for B 95-8 cells and still less for the M 81 cells. After a single passage in either lymphoblastic cell lines, they had a reduced oncogenicity in vivo. The polypeptide pattern analysis by PAGE showed some modifications.

Adenoviridae↗
PubMed1979

[Modification of human adenovirus type 7 after culture in guinea pig brain cells in vitro].

Multiplication of human adenovirus 7 (Ad7) is restricted in subcultures of adult Guinea Pig brain cells. It was however possible to recover enough infectious virus to purify it, and to study its properties. Several properties are similar to that of Ad 7 produced in HeLa cells but differences in sensitivity to cesium chloride and in its polypeptide composition were found.

Adenoviruses, Human↗
PubMed1978

[Decrease in stimulation and allogeneic response following experimental infection of mice with human adenovirus 5].

15 days after experimental infection with human adenovirus 5, C57 Bl/6 murine spleen cells were found to have altered in vitro properties. Proliferative response to phytohemagglutinine was slightly increased while the allogeneic response was decreased and the capability to stimulate allogeneic cells was significantly diminished. The degree of expression of surface antigens responsible for allogeneic stimulation is influenced by many regulatory factors, still poorly defined; alteration of some factors of antigenicity may result, directly or indirectly, from certain viral infections.

Adenoviruses, Human↗
PubMed1978

Interactions of Shope papilloma virus with some other DNA viruses.

The interactions of Shope papilloma virus (SPV) with primate and rabbit cells in tissue culture have been investigated. The rabbit cell cultures were derived from normal epidermis, from SPV-infected epidermis, from SPV-induced papillomas, and from an SPV-associated carcinoma. None of these cell cultures, whether infected in vitro with SPV or derived from tissues infected in vivo, ever produced infectious SPV or even detectable viral antigens. Some other DNA viruses behaved differently in cells which had been in previous contact with SPV either in vitro or in vivo. Adenovirus type 5 multiplies better in human cells infected 24 h previously with SPV than in the untreated controls. The production of infectious virions of either herpes simplex virus or Shope fibroma virus is reduced in cells derived from SPV-induced papillomas or carcinoma, due, apparently, to a defect in viral maturation. Of the rabbit cells, only those derived from in vivo infected tissue, or those previously infected in vitro with SPV, could be transformed by SV40. The rabbit cell lines derived from papillomas or carcinoma differed from their counterparts derived either from normal epidermis or from tissue infected 24 hrs before biopsy, in their karyotype, and in their ability to grow in soft agar. Similar karyotypic alterations were induced in cells derived from healthy epidermis by infection in vitro with SPV.

Adenoviruses, Human↗
PubMed1978

[Cultured cell isolates of the smooth muscle layer from the rat duodenum].

A culture obtained from rat duodenal smooth muscle layer is described. The cells were isolated by trypsinization (0.2%), and the medium used for culture was either MEM with glutamine and non-essential AA or RPMI, both containing 10% foetal calf serum. The cell culture contained both smooth muscle cells and fibroplasts in proportions varying with the age of the culture. At day 6, cell differentiation is important. At day 12, when the cells are confluent, the majority of the cells are fibroblasts. Although it is difficult, the transfer of cells is possible at least twice.

Animals↗
PubMed1977

Deoxyribonuclease activity associated with adenovirus 5 and 7.

A DNase activity was found associated with isolated components of adenovirus types 5 and 7. The enzymatic activity was associated with the purified virus and viral components extracted from the soluble material of infected cells. The DNase activity of adenovirus type 5 was maximum at pH 5.5 and 7.0 whereas adenovirus 7 was active only at pH 7.0. Both DNases were shown to be located in the penton fraction.

Adenoviruses, Human↗