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Biomedical subjects

Y Ben-Shaul

Publications and source records attributed to Y Ben-Shaul.

At least 55 records · Page 3Linked to original sources
PubMed1975

Actin-activated ATPase from human erythrocytes.

A fibrillar protein complex, possessing ouabain-insensitive Ca2+-ATPase activity was isolated from human erythrocyte membranes by using a low ionic strength extraction procedure. Mg2+-ATPase activity was revealed upon addition of rabbit skeletal muscle actin, thus demonstrating the presence of a myosin-like protein in the crude extract of the erythrocyte membrane. Upon sodium dodecylsulfate gel electrophoresis, the extract showed mainly the doublet of subunit molecular weight bands of 230 000 and 210 000, and more than 10 faster moving bands. Gel filtration of the erythrocyte membrane extract on Sepharose 4B furnished 4 fractions. Fraction I, containing the doublet and 80 000, 60 000 and 46 000 subunit molecular weight bands was 5-fold purified with respect to Ca2+-ATPase activity, but was devoid of actin-activated Mg2+-ATPase activity. Fraction II, containing only the doublet, was devoid of Ca2+ and actin-activated Mg2+-ATPase activity. The 210 000 subunit molecular weight protein could be phosphorylated in the presence of Mg2+ in the crude extract and Fraction I but not in Fraction II.

Actins↗
PubMed1975

Photosynthetic Reactions in the Marine Alga Codium vermilara: II. Structural Studies.

Chloroplasts from Codium vermilara, isolated by relatively crude methods, are able to fix CO(2) at rates comparable to the rates of intact plants. Sections in thalli of Codium vermilara show that the chloroplasts are surrounded by a thin layer of cytoplasm. This surrounding layer of cytoplasm, is retained also in isolated chloroplasts, and presumably preserves the intactness of the chloroplast envelope.

Journal Article↗
PubMed1973

In vitro production of mouse mammary tumor virus in a mouse mammary tumor ascites line.

An ascites line derived from a spontaneous mouse mammary carcinoma produces, on explantation and cultivation in vitro, large amounts of oncornavirus particles. The biochemical, biophysical, and electron microscopic characteristics of the virions are described. Molecular hybridization and immunological methods identify these virions as mouse mammary tumor virus.

Animals↗
PubMed1973

Separation and Ultrastructure of Proplastids from Dark-grown Euglena Cells.

A procedure for the separation of proplastids free of mitochondria from dark-grown Euglena cells has been developed. A fraction enriched in proplastids was used for freeze-etching study of proplastid structure. The prolamellar body in freeze-etched replicas appeared sponge-like, with thylakoids, often vesicular, emerging from it. The prolamellar body and the thylakoids were covered by particles of about 100A in diameter. No larger particles, typical of light-grown chloroplasts, were observed.

Journal Article↗